Roxburgh rose polysaccharide functionalized nanometer selenium compound, preparation method thereof and application of roxburgh rose polysaccharide functionalized nanometer selenium compound in blood glucose reducing drug

A functionalized, nano-selenium technology, applied in drug combinations, pharmaceutical formulations, sulfur/selenium/tellurium active ingredients, etc., to achieve the effects of narrow particle size range, inhibition of apoptosis, and increase of insulin secretion

Active Publication Date: 2019-02-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation of nano-selenium with the effect

Method used

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  • Roxburgh rose polysaccharide functionalized nanometer selenium compound, preparation method thereof and application of roxburgh rose polysaccharide functionalized nanometer selenium compound in blood glucose reducing drug
  • Roxburgh rose polysaccharide functionalized nanometer selenium compound, preparation method thereof and application of roxburgh rose polysaccharide functionalized nanometer selenium compound in blood glucose reducing drug
  • Roxburgh rose polysaccharide functionalized nanometer selenium compound, preparation method thereof and application of roxburgh rose polysaccharide functionalized nanometer selenium compound in blood glucose reducing drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of Rosa roxburghii polysaccharide RTFP-3

[0045] After cleaning the fresh Rosa roxburghii fruit, dry it in a blast drying oven at 60 °C, crush it, pass through a 60-mesh sieve, and formulate a solid-liquid ratio of 1:6 g / mL according to Rosa roxburghii dry powder and 95% ethanol (v / v). The mixture was heated to reflux at 70 °C for 4 h, filtered to obtain a residue, and heated to reflux and filtered three times. The degreased roxburghii powder was dried in a blast drying oven at 45 °C for 48 h. Add 150 g of processed Rosa roxburghii dry powder, add distilled water for hot water extraction, extraction temperature is 95°C, time is 3 h, solid-liquid ratio is 1:30 (the units of mass and volume are g and mL, respectively), and the extraction times 2 times; centrifuge at 5000 g for 10 min, collect the supernatant and concentrate it to 1 / 4 of the original volume by rotary evaporation at 55°C to obtain the concentrated solution of Rosa roxburghii polysacc...

Embodiment 2

[0046] Example 2 Preparation of Rosa roxburghii polysaccharide nano selenium solution (SeNPs)

[0047] 10 mL of sodium selenite solution with a concentration of 1 mM was mixed with the same volume of a certain concentration of Rosa roxburghii polysaccharide solution (0.25, 0.5, 1, 2, 3, 4 mg / mL) or distilled water, and then 60 mL of 6 The mM vitamin C solution was stirred evenly, and the reaction was shaken on a shaker at 100 r / min at 37 °C for 12 h, and then the reaction solution was transferred to a dialysis bag and dialyzed at 4 °C for 48 h. Finally, the dialyzed nano-selenium solution was freeze-dried to obtain Rosa roxburghii polysaccharide nano-selenium particles (RP3-SeNPs) and nano-selenium particles (SeNPs). The structure of nano-selenium was characterized by transmission electron microscope (TEM), high-resolution scanning electron microscope (SEM), laser particle size analyzer, energy spectrum analysis (EDS) and infrared spectroscopy (FT-IR).

Embodiment 3

[0048] Example 3 Preparation of Rosa roxburghii polysaccharide nano-selenium solution (SeNPs)

[0049] 10 mL of sodium selenite solution with a concentration of 2 mM was mixed with the same volume of a certain concentration of Rosa roxburghii polysaccharide solution (0.25, 0.5, 1, 2, 3, 4 mg / mL) or distilled water, and then 80 mL of 8 mM The vitamin C solution was stirred evenly and reacted on a shaker at 200 r / min at 37 °C for 24 h, and then the reaction solution was transferred to a dialysis bag and dialyzed at 4 °C for 72 h. Finally, the dialyzed nano-selenium solution was freeze-dried to obtain Rosa roxburghii polysaccharide nano-selenium particles (RP3-SeNPs) and nano-selenium particles (SeNPs). The structure of nano-selenium was characterized by transmission electron microscope (TEM), high-resolution scanning electron microscope (SEM), laser particle size analyzer, energy spectrum analysis (EDS) and infrared spectroscopy (FT-IR).

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Abstract

The invention discloses a roxburgh rose polysaccharide functionalized nanometer selenium compound, a preparation method thereof and application of the roxburgh rose polysaccharide functionalized nanometer selenium compound in a blood glucose reducing drug and belongs to the technical field of nanometer selenium preparation. The preparation method comprises the following steps of (1) uniformly mixing a sodium selenite solution and a roxburgh rose polysaccharide solution, then adding a vitamin C solution, and performing uniform stirring and oscillatory reaction; and (2) dialyzing and drying a reaction liquid obtained in the step (1) to obtain the roxburgh rose polysaccharide functionalized nanometer selenium compound. By taking sodium selenite as a selenium source, vitamin C as a reducer andthe roxburgh rose polysaccharide (RTFP-3) as a surface modifier, the nanometer selenium compound (RP3-SeNPs) with the functionalized surface is environmentally friendly prepared, a nanometer seleniumpolysaccharide compound with good stability, narrow particle size distribution and good dispersibility is prepared, and the use of the roxburgh rose polysaccharide functionalized nanometer selenium compound in preparing a drug for inhibiting islet beta cell damages is provided.

Description

technical field [0001] The invention belongs to the technical field of nano-selenium preparation, and in particular relates to a polysaccharide-functionalized nano-selenium compound of Rosa roxburghii and its preparation method and application in hypoglycemic drugs. Background technique [0002] With the development of society and economy and the improvement of people's living standards, the incidence of diabetes is increasing day by day, and its morbidity and mortality have ranked third among non-communicable diseases, especially the number of people with type 2 diabetes is increasing rapidly. One of the causes of type 2 diabetes is that the decrease in the number or function of human pancreatic islet cells leads to a decrease in insulin secretion, which disrupts the balance of blood sugar. At present, it has been found that free radicals are one of the important causes of islet β-cell damage and apoptosis. In normal human body, free radicals are in a state of dynamic balan...

Claims

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Application Information

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IPC IPC(8): A61K47/61A61K33/04A61P3/10
CPCA61K33/04A61K47/61A61P3/10
Inventor 扶雄汪磊李超黄强
Owner SOUTH CHINA UNIV OF TECH
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