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A patterned bacterial cellulose film and its preparation method and application

A bacterial cellulose film and patterning technology, applied in medical science, prosthesis, etc., can solve the problems of long culture time, low fidelity, limited pattern height of patterned cellulose film, etc., and achieve clear pattern , high pattern fidelity, simple and efficient preparation method

Active Publication Date: 2020-06-09
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention solves the technical problems of limited pattern height, low fidelity and long culture time of the patterned cellulose film in the prior art

Method used

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  • A patterned bacterial cellulose film and its preparation method and application
  • A patterned bacterial cellulose film and its preparation method and application
  • A patterned bacterial cellulose film and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation method of patterned polydimethylsiloxane (PDMS) functional sponge

[0031] (1) Preparation of the patterned positive mold: use a glue spreader to evenly spread a layer of SU-8 photoresist (GM1070) on the dried silicon wafer, and set the number of revolutions of the glue spreader: the starting speed is 500r / min, the time is 40S; the speed of the second step is 2300r / min, and the time is 60S. A SU-8 coating with a thickness of about 25 μm was thus obtained on the silicon wafer. The silicon wafer is pre-baked, exposed (ultraviolet), post-baked, developed with a developer, and hardened to obtain the SU-8 positive mold. The pattern of the male mold can be square, strip, circle, etc.

[0032] (2) Preparation of PDMS sponge model: PDMS (Sylgard 184) prepolymer and curing agent (Sylgard 184) were mixed at a mass ratio of 10:1 and stirred evenly. Take the mixture of PDMS pre-polymerization liquid and curing agent, water, and liquid paraffin oil ...

Embodiment 2

[0033] Embodiment 2: the preparation method of patterned polydimethylsiloxane (PDMS) functional sponge

[0034] (1) Preparation of the male mold of the millimeter-scale channel: cut capillaries with a diameter of 2 mm into appropriate lengths, arrange them evenly and fix them on the silicon wafer with secondary polishing, and use them as the male mold of the millimeter-scale channel.

[0035] (2) Preparation of PDMS sponge model: PDMS (Sylgard 184) prepolymer and curing agent (Sylgard 184) were mixed at a mass ratio of 10:1 and stirred evenly. Take the mixture of PDMS pre-polymerization solution and curing agent, water, and liquid paraffin oil and carry out thorough mixing, stirring and emulsification according to the mass ratio of 5:35:7.5. Pour the emulsified mixed solution on the surface of the male mold (thickness is 2-3mm), let it stand for curing first, then put it into an oven at 65°C to heat and cure for 2 hours, and after the PDMS mixture is cured, separate it from th...

Embodiment 3

[0036] Embodiment 3: the preparation method of the bacterial cellulose membrane of patterning

[0037](1) The micron-scale patterned PDMS sponge model obtained in Example 1 is sterilized and placed on the surface containing Acetobacter glucosus culture (preferably the culture medium is inoculated at a volume ratio of 10:1 to the strain), as figure 1 shown.

[0038] (2) Place the culture bottle in a sterile incubator for 12-48h (30°C), and tear off the PDMS sponge model to obtain a patterned cellulose membrane, such as image 3 As shown, the prepared micron-scale strip patterned cellulose film.

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Abstract

The invention discloses a patterned bacterial cellulose membrane, and a preparation method and application thereof, and belongs to the technical field of polymer materials and biological manufacturing. The preparation method comprises the steps of: fully mixing a prepolymer A of polydimethylsiloxane with a curing agent B, then adding an organic solvent, then adding water dropwise, stirring the mixture, allowing the mixture to stand to allow the mixture to be initially solidified, heating and solidifying the mixture, removing the organic solvent and water to obtain water-emulsified polydimethylsiloxane functional sponge, floating the patterned PDMS sponge model on the surface of a culture liquid capable of producing bacterial cellulose, and performing fermentation culture for 12-48 h to obtain the patterned bacterial cellulose membrane. The method for preparing the patterned bacterial cellulose membrane by utilizing the PDMS sponge has characteristics of a simple process, short fermentation time, high pattern fidelity, and capability of allowing the pattern height to be 10 [mu]m to 2 mm, and the membrane has good biocompatibility and is an ideal environmentally friendly biomedical material.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and bio-manufacturing, and in particular relates to a patterned bacterial cellulose film and its preparation method and application. Background technique [0002] Bacterial cellulose is a kind of nanocellulose produced by Acetobacter, Agrobacterium, Rhizobium and Sarcina, etc. It is abundant in nature and biodegradable natural polymer. Due to its durability and low inflammatory response in the body, it is increasingly used as a medical material, such as breakthroughs in wound treatment, vascular grafts, etc., and has been applied clinically. In addition, large-scale production has been realized in papermaking, food, screen and high-end audio products. [0003] Surface-structured biomaterials can effectively guide cells in contact and have been applied to the regeneration and reconstruction of differentiated tissues. At present, there have been many reports on the production of surface-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08L83/04A61L27/20A61L27/50
CPCA61L27/20A61L27/507C08J9/28C08J2383/04
Inventor 刘笔锋张雷成冯晓均杜伟
Owner HUAZHONG UNIV OF SCI & TECH
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