Heat-resistant enzymic preparation as well as preparation method and application thereof

A technology of enzyme preparation and porous adsorption material, applied in the field of heat-resistant enzyme preparation and its preparation, can solve the problems affecting the use effect of enzyme preparation, denaturation and inactivation of enzyme molecules, etc., achieve good biocompatibility and improve storage stability , the effect of blocking adverse effects

Pending Publication Date: 2019-03-19
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The typical extrusion puffing method forms a pre-extrusion paste by adding steam and water. After conditioning, various powder materials are directly sent into the extrusion chamber of the extruder, and additional steam and water are introduced. Under the action of cutting force and heating, it is cooked and cooked, and the heating temperature causes most of the starch to gelatinize, and the enzyme molecules in the enzyme preparation are also denatured and inactivated, which ultimately affects the use effect of the enzyme preparation.

Method used

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  • Heat-resistant enzymic preparation as well as preparation method and application thereof
  • Heat-resistant enzymic preparation as well as preparation method and application thereof
  • Heat-resistant enzymic preparation as well as preparation method and application thereof

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[0043] The present invention also provides the preparation method of the enzyme preparation, comprising the following steps: 1) mixing the enzyme molecule solution with the pulverized porous adsorption material, and absorbing to obtain the porous adsorption material adsorbed with enzyme molecules; 2) in step 1) The surface of the porous adsorption material adsorbed with enzyme molecules described in is coated with a polysaccharide layer, and after vacuum treatment, it is dried to obtain a core material; 3) the core material described in step 2) is coated with a hydrophobic layer to obtain a hydrophobic layer-coated core 4) In step 3), the core material coated with the hydrophobic layer is coated with the outer wall layer, and dried to obtain the enzyme preparation.

[0044] In the invention, the enzyme molecule solution is mixed with the pulverized porous adsorption material for adsorption to obtain the porous adsorption material adsorbed with enzyme molecules. In the present ...

Embodiment 1

[0055] Take 10g of the quartz porous material and pulverize it to 80 mesh, and set it aside. Take 100mL of β-glucanase fermentation broth with an enzyme activity of 14600U / g, centrifuge at 3000rpm, 4°C for 10min, remove the precipitate, collect the supernatant and mix it with the pulverized quartz porous material, and centrifuge at 0.06MPa, 45°C Vacuum treatment for 20 minutes under certain conditions, to obtain a quartz porous material adsorbed with enzyme molecules.

[0056] Take 60 g of the quartz porous material adsorbed with enzyme molecules and suspend it in the fluidized bed. Take 3.2g of gum arabic and 6.4g of maltodextrin to prepare 48mL of polysaccharide aqueous solution, and coat the quartz porous material adsorbed with enzyme molecules at a spray speed of 1.2mL / min; after the coating is completed, keep the flowing state for 5min; Vacuum-process the above-mentioned material in wet state for 20min under the conditions of 0.06MPa and 45°C. After vacuum treatment, dr...

Embodiment 2

[0062] Take 4g of sepiolite and crush it to 80 mesh, set aside. Take 100mL of the fermented supernatant β-glucan enzyme solution with an enzyme activity of 14600U / g, mix it with the crushed sepiolite above, and treat it under vacuum at 0.06MPa and 45°C for 5min. Centrifuge at ℃ for 10 min, remove the supernatant, and obtain sepiolite with enzyme molecules adsorbed thereon.

[0063] Take 80 g of the porous adsorption material adsorbed with enzyme molecules and suspend it in the fluidized bed. Take 3.75g of gum arabic and 3.75g of maltodextrin to prepare 62.5mL of polysaccharide aqueous solution, and coat the porous adsorption material with enzyme molecules at a spray speed of 1mL / min; after the coating is completed, add 2g of talcum powder to keep Flowing state for 5 minutes; the above material in wet state was vacuum treated for 15 minutes under the conditions of 0.04MPa and 40°C. After vacuum treatment, dry at 40°C and normal pressure for 15 minutes, then transfer to a vacu...

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Abstract

The invention provides an enzymic preparation as well as a preparation method and application thereof, which belongs to the technical field of enzymic preparations. The enzymic preparation comprises acore material as well as a hydrophobic layer and an outer wall layer covering the core material; the core material comprises enzyme molecules, a porous absorption material and a polysaccharide layer,the enzyme molecules are absorbed inside the porous absorption material, and the polysaccharide layer is absorbed on the surface of the porous absorption material; the hydrophobic layer material is selected from one or more of sodium stearate, cetyl trimethyl ammonium bromide, alpha-cyclodextrin and polylactic acid; the outer wall layer is selected from one or more of gelatin, glycerinum, sodiumalginate and polysaccharide; and the weight ratio of the core material to the hydrophobic layer and the outer wall layer is (80 to 110): (0.3 to 0.7): (2.00 to 4.00). The enzymic preparation is good in heat stability and good in processing resistance.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparations, in particular to a heat-resistant enzyme preparation and its preparation method and application. Background technique [0002] Enzymes are proteins or RNAs produced by living cells that have high specificity and high catalytic efficiency for their substrates. Enzymes are an extremely important class of biocatalysts. The main function of enzyme preparation is to catalyze the product processing process and improve the processing method. At present, enzyme preparations have been used in various fields such as feed, agriculture, medicine and health care, and food. The efficacy of enzyme preparations has a significant correlation with the configuration of enzyme molecules. [0003] Most of the existing enzyme preparations are processed by coating after fermentation, and the production methods mainly adopt methods such as fluidized bed drying, spray drying and freeze drying. Freeze-dryi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96A23K20/189A23K20/163A23K20/105A23K20/158A23K20/28A23K40/30A23K50/80A23K50/30A23K40/10
CPCC12N9/96A23K20/105A23K20/158A23K20/163A23K20/189A23K20/28A23K40/10A23K40/30A23K50/30A23K50/80
Inventor 董颖超秦玉昌李军国吕学泽杨洁谷旭牛力斌
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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