Application of kobutimycin B in preparation of bacterial or fungal inhibitor
A technology of fungi and bacteria, applied in the field of agricultural microbiology, can solve the problems of banning and destroying, and achieve the effect of strong antagonistic ability
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Embodiment 1
[0018] Determination of the Minimum Inhibitory Concentration of Cobutidin B against Solanaceae Ralstonia solanacearum by Tube-and-Dish Method
[0019] Principle: Deduced according to the law of diffusion, the logarithmic value of the total amount of antibiotics is linearly related to the square of the antibacterial radius. When the antibiotic concentration reaches or exceeds the MIC value, the test bacteria are inhibited and cannot reproduce, thus presenting a transparent bacteriostatic zone.
[0020] LogY=kX 2 +b (Y is the concentration, X is the radius of the inhibition zone)
[0021] 1) Solanaceae R. solanacearum was streaked and activated to obtain a single colony, and the single colony was picked and inoculated into SPA medium at 28°C and 150rpm and cultivated until the logarithmic growth phase.
[0022] 2) Cobutidin B with concentrations of 7.1, 14.2, 28.4, 56.8, and 113.6 μg / mL was prepared, and water was used as a negative control.
[0023] 3) Add 1% pathogenic bact...
experiment example 2
[0026] Determination of anti-common pathogenic bacteria activity of cobutidin B by tube-and-disk method:
[0027] 1) After the pathogenic bacteria Xanthomonas oryzae (Xanthomonas oryzae), Pseudomonas syringae (Pseudomonassyringa e) and Xanthomonascitri (Xanthomonascitri) were activated, a single colony was picked and inoculated in LB medium at 28°C and cultivated at 150rpm until number of growing seasons. Pathogen Staphylococcus aureus (Staphylococcus aureus) was cultured at 28° C., 150 rpm until logarithmic growth phase.
[0028] 2) Cobutidin B at a concentration of 100 μg / mL was prepared, and water was used as a negative control.
[0029] 3) Add 1% pathogenic bacteria to the melted solid medium (1ml bacterial solution + 99ml medium) (add when the shaker flask is not hot), place an Oxford cup on the inverted plate and add 100ul butycin B, repeat 3 times , Staphylococcus aureus was cultured at 37°C, and Xanthomonas oryzae, Xanthomonas japonica, and Canker sorghum citrus were...
experiment example 3
[0035] Determination of Corbutycin B's Activity Against Common Pathogenic Fungi by Punching Method
[0036] 1) Pathogenic fungi Fusarium jsolani, Fusarium oxysporum, Verticillium dahliae, Magnaportheoryzae, Borrytisciner ea, Gibberella wheat The fungus (Fusarium graminearum), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), and Penicillium italicum (Penicillium italicum) were activated, and the hyphae were picked and inoculated into PDA medium for cultivation at 28°C.
[0037] 2) Cobutidin B at a concentration of 100 μg / mL was prepared, and water was used as a negative control.
[0038] 3) Pick the bacterial block in the center of the PDA solid medium plate, punch 6 holes equidistantly with a hole punch (the diameter of the hole is 4mm), add 20μl cobutidin B, repeat 3 times, and culture at 28°C , and the observation results are shown in Table 2.
[0039] Table 2: Corbutin B Antifungal Spectrum
[0040]
[0041] +: Indicates that there is an effect, -: Indicates that ...
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