Microbial preparation and application thereof to aquaculture

A microbial agent and aquaculture technology, applied in the field of microorganisms, can solve the problems of destroying the micro-ecological balance of the normal microbiota in the epidermis and intestinal tract of farmed animals, exacerbating the problems of farmed animal diseases, and the quality and safety of aquatic products, achieving good application and development prospects. Wide range of prevention and control, high safety effect

Inactive Publication Date: 2019-03-29
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control methods for bacterial aquatic diseases in my country still mainly rely on chemical drugs such as antibiotics. Antibiotic treatment and other measures can achieve the purpose of partially controlling the occurrence of diseases and promoting the growth of farmed animals, but the resulting drug resistance of pathogenic microorganisms and drug residues , water environment pollution, and aquatic product quality and safety issues are becoming more and more serious; at

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The isolation of embodiment 1 bacillus licheniformis

[0017] In December 2014, the Bacillus licheniformis strain was isolated from the intestinal contents of wild wild boars in Tantou Town, Longhui County, Hunan Province. The specific method is: take the intestinal contents of wild wild boar and add it to a conical flask filled with 10 mL of sterilized water, shake and incubate for 30 min (200 r / min), then take 1 mL of the suspension and place it in 9 mL of sterile water to make 100 mL of sterile water. -2 Dilute the suspension, and so on, to make 10 -5 , 10 -6 Dilute the suspension; draw 100 μL each, add it to the nutrient broth medium plate, MRS medium, and PDA medium, and spread it evenly with a coating bar. Each treatment is repeated 3 times. Place the above culture dish in a constant temperature incubator at 37°C for 1-2 days (PDA medium is cultured at 30°C). Select a single colony and transfer it to the corresponding medium for culture. After the colony grows,...

Embodiment 2

[0019] Example 2 Bacillus licheniformis to the in vitro antibacterial effect of common pathogenic bacteria

[0020] The specific experimental method is as follows:

[0021] 1, YHSH-02 The preparation of bacterial suspension: get slanted seeds, inoculate in 50ml sterilized TSB liquid medium, shake and cultivate at constant temperature for 18h (cultivation temperature 30 ℃, rotating speed 200rpm); bacterium suspension 8ml in 10ml sterilized centrifuge tube, Reserved, all are made and used now.

[0022] 2. Preparation of bacterial suspensions of pathogenic Vibrio such as Vibrio anguillarum

[0023] (1) Pick a ring of bacterial lawn from the inclined surface and inoculate it into 50m1 TSB liquid medium, and culture it on a shaking table for 18 hours (cultivation temperature 30°C, rotation speed 140rpm);

[0024] (2 Take 5 ml of the above-mentioned bacterial suspension and re-inoculate it into 50 ml of newly prepared TSB liquid medium, and cultivate it on a shaking table for 18...

Embodiment 3

[0033] Embodiment 3 The preparation of microbial bacterial agent (Bacillus licheniformis preparation) of the present invention

[0034] 1. Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 37°C for 24 hours. The seeds are inoculated with 5% inoculation amount into a 5L large triangle flask with a liquid volume of 2LLB medium, shaken and cultured at 37°C for 20-28 hours, and the concentration of the bacteria solution is tested. The viable bacteria content exceeds 2 billion / ml, and it is ready As a liquid strain of Bacillus licheniformis;

[0035] 2. Use the Bacillus licheniformis liquid cultured in step 1 as seeds, inoculate into 600L fermentation medium, the inoculation amount is 10%, stir at 120r / min, the air volume per minute liquid-gas ratio is 1:0.8, and cultivate at 37°C ...

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Abstract

The invention discloses a microbial preparation, and specifically discloses a microbial preparation prepared from pig-derived bacillus licheniformis YHSH-02, and an application of the microbial preparation to aquaculture. The strain disclosed by the invention is preserved in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms at No. 3, No. 1 yard, Beichen West Road, Chaoyang district, Beijing, the preservation number is CGMCC NO.15454, and the preservation date is 15th March, 2018. The bacillus licheniformis YHSH-02 disclosedby the invention is separated from intestines of wild boars, can produce rich small peptide, lipopeptide type bacillin, antibacterial protein and the like, can restrain growth of various aquaculture pathogenic bacteria of vibrio parahaemolyticus, edwardsiella tarda, aeromonas hydrophila, pseudoalteromonas nigrifaciens, vibrio vulnificus and the like, and can be used for preventing and treating aquaculture diseases caused by various bacteria of the vibrio parahaemolyticus, the edwardsiella tarda, the aeromonas hydrophila, the pseudoalteromonas nigrifaciens, the vibrio vulnificus and the like. The situation that the microbial preparation can effectively prevent and treat black-foot diseases of prawns is found for the first time, an effective scheme is provided for preventing and treating theblack-foot diseases of the prawns, and the microbial preparation has important significance. The bacillus licheniformis YHSH-02 obtained from the intestines of the wild boars is a bacterial strain which is high in preventing and treating efficiency, wide in preventing and treating range and notable in growth promotion and has biological preventing and treating potential, and has favorable application and development prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, discloses a microbial agent and its application in aquaculture, in particular discloses a microbial agent produced by Bacillus licheniformis Bacillus licheniformis YHSH-02 Microbial agent prepared by fermentation and its application in aquaculture. Background technique [0002] In recent decades, feed drug additives based on antibiotics, hormones, and preservatives have brought great benefits to the production of aquaculture such as fish and shrimp. However, with its wide application, some disadvantages have also appeared, such as the emergence of drug-resistant strains, antibiotic residues in livestock, poultry, fish, shrimp and aquatic products, and superinfection. These issues have attracted widespread attention and concern from the international community. , More and more countries have banned or restricted various antibiotics, hormones, and preservatives as feed additives. The appli...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P31/04C12R1/10
CPCA61K2035/115A61P31/04C12N1/20C12N1/205C12R2001/10
Inventor 蒋常德蒋常银黄进
Owner FOSHAN YANHUI BIOTECH CO LTD
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