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A kind of industrial production method of intestinal probiotic preparation

A technology of intestinal probiotics and preparations, which is applied in the field of microbial preparations, can solve the problems of large amount of auxiliary protection carrier and reduce the relative content of viable bacteria in probiotic preparations, so as to prolong the storage time, increase the number, and increase the number of viable bacteria Effect

Active Publication Date: 2019-11-12
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to add a large amount of protective agent during use, which reduces the relative content of viable bacteria in the probiotic preparation
Chinese patent publication CN102614225A discloses a compound live probiotic preparation and its industrial production method. The compound live probiotic preparation is formed by mixing Lactobacillus acidophilus A-10, Bifidobacterium adolescent B-13, and an auxiliary protective carrier , wherein the compound probiotic powder is 2.0%-30%, and the auxiliary protective carrier is 70-98%. Although this invention has improved the number of viable bacteria and the survival rate of probiotics to a certain extent, there is still the amount of auxiliary protective carrier big problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of industrial production method of intestinal probiotics preparation

[0029] (1) Inoculate Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, and Lactobacillus delbrueckii subsp. bulgaricus into different fermentation media for fermentation and culture respectively. When the number of viable bacteria in the fermentation culture reaches 4.5× 10 9 cfu / mL, the fermentation culture was terminated, and the fermentation culture liquid was obtained, wherein the culture temperature was 30°C, and the formula of the fermentation medium was: glucose 15.0g, trypsin hydrolyzed casein 2.0g, yeast extract 5.5g, citric acid Sodium 1.0g, dipotassium hydrogen phosphate 2.5g, magnesium sulfate 0.1g, L-cysteine ​​hydrochloride 0.1g, tomato juice 2.0g, Tween-20 2.0mL, add water to 1000mL, pH 6.0, 105 Sterilize at ℃ for 15 minutes;

[0030] (2) Inoculate Streptococcus thermophilus into the fermentation medium and carry out fermentation culture, when...

Embodiment 2

[0033] Embodiment 2 A kind of industrial production method of intestinal probiotics preparation

[0034] (1) Inoculate Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, and Lactobacillus delbrueckii subsp. bulgaricus into different fermentation media for fermentation and culture respectively. When the number of viable bacteria in the fermentation culture reaches 3× 10 9 cfu / mL, the fermentation culture was finished, and the fermentation culture liquid was obtained, wherein, the culture temperature was 45°C, and the formula of the fermentation medium was: glucose 5.0g, trypsin hydrolyzed casein 15.0g, yeast extract 1.5g, sodium citrate 5.0g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.75g, L-cysteine ​​hydrochloride 0.001g, tomato juice 25g, Tween-20 0.5mL, add water to 1000mL, pH 7.0, extinguish at 105℃ Bacteria for 15 minutes;

[0035] (2) Inoculate Streptococcus thermophilus into the fermentation medium and carry out fermentation culture. Wh...

Embodiment 3

[0038] Embodiment 3 A kind of industrial production method of intestinal probiotics preparation

[0039] (1) Inoculate Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum, and Lactobacillus delbrueckii subsp. 10 9 cfu / mL, the fermentation culture was finished, and the fermentation culture liquid was obtained, wherein the culture temperature was 40°C, and the formula of the fermentation medium was: glucose 10.0g, trypsin hydrolyzed casein 8.0g, yeast extract 4.2g, sodium citrate 4.0g, dipotassium hydrogen phosphate 2.1g, magnesium sulfate 0.5g, L-cysteine ​​hydrochloride 0.006g, tomato juice 15g, Tween-20 1.0mL, add water to 1000mL, pH 6.5, extinguish at 105℃ Bacteria for 15 minutes;

[0040] (2) Inoculate Streptococcus thermophilus into the fermentation medium and carry out fermentation culture, when the number of viable bacteria in the fermentation broth reaches 2.3×10 9 cfu / mL, add porous starch to the fermentation broth, so that the initial content of p...

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Abstract

The invention provides an industrial production method of an intestinal probiotic preparation, belonging to the technical field of microbial preparations. The production method comprises the following steps: (1) respectively fermenting and culturing Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum and Lactobacillus delbrueckii subsp. Streptococcus thermophilus is fermented and cultured, and porous starch is added to the fermentation broth before the end of fermentation to obtain a fermentation broth; (3) The fermentation broth of various strains obtained in steps (1) and (2) is collected to obtain active wet sludge , mix the wet fungus slime with the protective agent and distilled water evenly; (4) go through the granulator, spheronizer, dryer, and coating machine for granulation, spheronization, low-temperature vacuum drying, and coating, and the sausage can be obtained after passing the test. Probiotic preparations. Using the method of the invention can increase the quantity of viable bacteria in the probiotic preparation, prolong the storage time of the probiotic preparation, and reduce production costs.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to an industrial production method of intestinal probiotics. Background technique [0002] With the development of social economy and the change of natural environment, the pace of life is accelerated, and various pressures are coming one after another. People have various health problems. Probiotics are closely related to human health. Probiotics are a class of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Probiotics can be roughly divided into three categories, including Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jansnii, Lactobacillus Lamansii, etc.), B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135C12R1/01
CPCA23L33/135C12N1/20A23V2002/00A23V2400/125A23V2400/175A23V2400/137A23V2400/517
Inventor 梁金钟肖雯娟林凤祥
Owner 哈尔滨美华生物技术股份有限公司