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Bacillus tequilensis XG18 and applications thereof

A technology of Bacillus tequila and Bacillus, applied in application, bacteria, fungicides and other directions, to achieve good development and application prospects, strong colonization, and strong antagonistic effects

Inactive Publication Date: 2019-04-12
HUBEI ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Yet, about Tequila bacillus can be on the research report on multiple plant pathogenic bacterial disease and fungal disease seldom at the same time, the present invention will protect this bacterium in effectively preventing and treating by Xanthomonas pathogenic bacteria and Pseudomonas syringae Use in plant diseases caused by bacilli

Method used

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  • Bacillus tequilensis XG18 and applications thereof
  • Bacillus tequilensis XG18 and applications thereof
  • Bacillus tequilensis XG18 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening and identification of Bacillus tequilensis XG18

[0032] 1. Screening of Bacillus tequilensis XG18

[0033] a. Separation and purification of bacillus:

[0034] Take 10g of the rhizosphere soil sample of orchid plants suffering from Botrytis cinerea and add it to a conical flask filled with 90mL sterile water and 30-50 glass beads to make a suspension with a concentration of 1:10. Shake at 28°C and 180rpm After shaking in the bed for 20-30min, place the bacterial suspension in a water bath at 75-85°C for 12-18min. Transfer 1 mL of the soil suspension into a test tube filled with 9 mL of sterile water to make 10 -2 Bacterial suspension, and so on, get 10 -3 , 10 -4 , 10 -5 and 10 -6 bacterial suspension. After serial dilution, pipette 100 μL for a dilution factor of 10 -3 -10 -6 The bacterial suspension was evenly spread on the separation medium - beef extract peptone medium, the medium formula is as follows (1L): beef extract 3.0g, peptone 1...

Embodiment 2

[0046] Utilize the method for preparing biocontrol agent by Bacillus tequilensis XG18:

[0047] Pick a circle of XG18 colony on LB plate or slant and transplant it into the test tube for activation, and cultivate it on a shaker at 35°C for 16 hours, with a rotation speed of 150rpm. The LB liquid medium in the test tube is: peptone 10g / L, yeast extract 5g / L, chlorinated Sodium 5g / L, pH 7.0; the activated bacterial solution was transplanted into a 250mL Erlenmeyer flask containing 100mL LB liquid medium according to the inoculation amount of 1%-3%, at a rotation speed of 150rpm, and cultured on a shaker at 35°C for 10h-24h, When the bacterial content is greater than 2×10 8 cfu / mL or more can obtain the fermentation broth, which can be stored as a biocontrol agent.

Embodiment 3

[0048] Example 3 Antagonism of Bacillus tequilensis XG18 to Xanthomonas pathogenic bacteria

[0049] 1. Take a single ring of each of the three Xanthomonas colonies on the LB plate and place them in sterilized liquid LB medium, and culture them on a shaker at 28°C for 24-48h at a speed of 150rpm to obtain Xanthomonas campestris The fermentation broth of three pathogens: Xanthomonas campestrispv.Campestris(Xcc), X.oryzae pv.Oryzae(Xoo), and X.axonopodis pv.Citri(Xac);

[0050] 2. Take a single colony of Bacillus tequilensis XG18 on the LB plate and put it in a sterilized liquid LB medium, and culture it on a shaker at 35°C for 12 hours at a speed of 150rpm to obtain the fermentation broth of the antagonistic bacteria Bacillus tequilensis XG18;

[0051] 3. Take 100 μL of each of the three pathogenic bacteria fermentation liquids in step 1, spread them on the LB solid medium respectively, and then use a sterile puncher to evenly punch six 7mm holes on the LB solid medium, of whic...

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Abstract

The present invention discloses bacillus tequilensis XG18 and applications thereof. The strain is bacillus tequilensis XG18, CCTCC No: M2018831. The bacillus tequilensis XG18 is a strain which has antagonism to xanthomonas, pseudomonas syringae and botrytis cinerea. The bacillus tequilensis can inhibit a plurality of bacterial diseases and fungal diseases simultaneously, has broad-spectrum antagonism, has a fast growth rate, greatly shortens the cultivation time for general bacteria and fungi, reduces the production cost, and has good development and application prospects.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a Bacillus tequilensis XG18 and its application. Background technique [0002] Xanthomonas bacteria are a class of Gram-negative plant pathogens that can infect more than 400 kinds of plants, including important food crops and economic crops, such as: rice, cotton, wheat, soybeans, citrus, bananas, etc. resulting in reduced production and significant economic losses, see (Leyns F, De Cleene M, Swings J, De Ley J. The host range of the genus Xanthomonas. The Botanical Reviews, 1984, 50(3):308-355). Xanthomonas campestris (X. campestris pv. Campestris, Xcc) can infect all cruciferous plants and cause black rot. Rice bacterial blight (X.oryzae pv.Oryzae, Xoo) can cause rice bacterial blight, and rice is the staple food of 2.7 billion people in the world. Rice bacterial blight is the three most serious diseases of rice For one, see (John M, Stephane G, Shimpei M, et al. Top 10 plant...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 魏蜜陈嘉敏刘惠英张志林
Owner HUBEI ENG UNIV
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