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Preparation and application of a light-to-heat conversion lattice array chip

An array chip, photothermal conversion technology, applied in the field of biomedical technology and engineering, can solve problems such as lack of 2D plasma, and achieve the effects of increased sensitivity, easy connection, and stable properties

Active Publication Date: 2020-10-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is a lack of 2D plasma "hot spot" enhanced detection technology for the combination of targeted tumor cells and photothermal therapy. With the help of gold nanoparticles 2D plasma long-range effect amplification phenomenon, the development of high-sensitivity, targeted photothermal conversion array chip devices

Method used

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  • Preparation and application of a light-to-heat conversion lattice array chip
  • Preparation and application of a light-to-heat conversion lattice array chip
  • Preparation and application of a light-to-heat conversion lattice array chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1 polydendrite gold particle material

[0045] Prepare the gold stock solution as follows: Stir and add 200ml of 0.75mM HAuCl in the distillation apparatus 4 Solution, quickly add 5ml of 155.2mM trisodium citrate solution at one time, the color of the solution changes to deep red rapidly, continue to heat and reflux the solution for 15min, and collect the gold seed solution. Add 600ul of 25mM HAuCl to 20ml of aqueous solution containing 100ul of gold seed stock solution 4 , 50ul of 1M HCL, while stirring vigorously at 1800rpm, 100ul of 10mM silver nitrate and 200ul of 0.1M ascorbic acid were added dropwise for 10s. A multi-branched gold particle material is prepared.

[0046] The gold particle material obtained is dripped on the carbon film at a frequency of 10 microliters each time for three minutes to prepare a sample, and observe the transmission electron microscope (such as figure 1). After testing with a 980nm laser and an infrared...

Embodiment 2

[0048] Example 2 Gold particle material quenches fluorescent carbon quantum dots

[0049] Incubate the gold particle material and fluorescent carbon quantum dots at a ratio of 1:1 at 4°C for 12h, centrifuge at 10,000rpm for 10min, wash 3 times with water, and then test the fluorescence (such as image 3 ). exist image 3 Among them, the gold material has no absorption and no fluorescence, but only the fluorescent carbon quantum dots have fluorescence, and the intensity is 11125. The fluorescence value of the gold composite fluorescent carbon quantum dots is 1360, which is reduced by 10 times, indicating that the gold particle material made by the method of the present invention has Quenching the fluorescence effect.

Embodiment 3

[0051] The invention modifies the gold particle material for the glassy carbon electrode.

[0052] Activate the electrode with 0.5% sulfuric acid, and the electrolyte measures the signal of the glassy carbon electrode, (K 3 [Fe(CN)] 6 0.0823g, K 4 [Fe(CN)] 6 .3H 2 O 0.1056g, KCl 0.7455g), after cleaning, the gold particle material is electroplated and deposited on the glassy carbon electrode, and the electroplating time is 100s. The sensitivity of the modified glassy carbon electrode was verified by the CV response of the bare electrode (unmodified glassy carbon electrode) and the modified electrode. Such as Figure 4 As shown, the current value displayed by the bare electrode is 1.24E-4, and the electrode displayed by the modified gold particle material is 5.6E-4. It can be seen that the modified gold particle material has the characteristics of enhancing the sensitivity of the electrode, which can make The sensitivity of the electrode is enhanced by 4-5 times, which i...

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Abstract

The invention discloses preparation and application of photothermal conversion lattice array chips, and belongs to the field of biomedical technology and engineering. Prepared multi-dendritic gold nanoparticles with a photothermal conversion effect are applied to lattice array chips, and have the characteristics of high sensitivity and stable property; and through replacement of such marks as antibodies, enzymes, DNAs and RNAs on the gold nanoparticles connected to the chips, multiple substances or tumor cells can be identified and treated, and greater application prospect is achieved.

Description

technical field [0001] The invention relates to the preparation and application of a photothermal conversion lattice array chip, belonging to the field of biomedical technology and engineering. Background technique [0002] Photothermal therapy (photothermal therapy, PTT) is a new type of local tumor treatment method that has developed rapidly in recent years. ) stimulated by photothermal effect (hyperthermia), induced acute tumor necrosis, apoptosis and immune response, etc., so as to achieve the inhibitory effect on tumors. The key to the efficacy of PTT therapy lies in the temperature gradient changes inside the tumor cells and the changes in the surrounding tissues. When the temperature is in the range of 37-41°C, it can cause accelerated blood flow and increase the permeability of cell membrane; when the temperature is in the high temperature range of 41-48°C, it can cause protein folding and deformation, increase the sensitivity of radiotherapy and chemotherapy, and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B22F1/00C25D5/54C23C14/35C23C14/18C23C14/24C23C14/12G01N33/50
Inventor 皮付伟尹子叶刘玲张圆媛李颖李文飞孙秀兰
Owner JIANGNAN UNIV