cp4-epsps monoclonal antibody and preparation method thereof

Abstract

The invention provides a CP4-EPSPS monoclonal antibody and belongs to the technical field of bioengineering. Existing problems of high preparation cost of CP4-EPSPS monoclonal antibody and the like are solved. The CP4-EPSPS monoclonal antibody comprises a heavy chain and a light chain, wherein the nucleotide sequence of the heavy chain is shown in a sequence table (1), and the nucleotide sequenceof the heavy chain can encode the amino acid sequence of the heavy chain shown in a sequence table (2). The light chain nucleotide sequence is shown in a sequence table (3) and can encode the light chain amino acid sequence shown in a sequence table (4). The CP4-EPSPS monoclonal antibody has the advantages of low cost and the like.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a CP4-EPSPS monoclonal antibody and a preparation method of the CP4-EPSPS monoclonal antibody. Background technique [0002] Glyphosate (N-phosphonomethyl glycine) is a broad-spectrum herbicide that can control a variety of annual and perennial monocotyledonous or dicotyledonous weeds and herbaceous plants. , low dosage per unit area, easily degraded in the soil environment and harmless to the environment, etc., glyphosate has become a herbicide with the largest usage and the largest promotion area in the world. Although glyphosate is the best choice as a herbicide, because it is a non-selective herbicide, it will cause harm to weeds and crops, affecting the growth and yield of crops. For this reason, it has become the mainstream of research to introduce glyphosate-resistant CP4-EPSPS gene through transgenic technology into the plant genome to form glyphosate-resistant tr...

AI Technical Summary

Problems solved by technology

However, due to the small amount of ascites produced by a single mouse and the large individual differences, the obtained anti-glyphosate gene protein CP4-EPSPS monoclonal antibody has large differences between batches. Discrepancy issues make detection less accurate

In order to solve the above problems, another method is to prepare antibodies through CHO cell expression, which can avoid the use of a large number of mice and ensure that the differences between different batches of antibodies are small, but the demand for antibodies is limited, and cell expression is continuous. The cost of artificially controlling cell expression of antibodies is high, which severely limits the preparation of monoclonal antibodies

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