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Probiotic composition and use thereof

A composition and technology of probiotics, applied in the field of biomedicine, can solve the problems of few types of probiotics, less research on compound probiotics, unfavorable recovery of vaginal flora, etc., and achieve the effect of inhibiting vaginal flora

Active Publication Date: 2021-03-23
HANGZHOU GRAND BIOLOGIC PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types of probiotics used in the lactic acid bacteria vaginal capsule are too small, which is not conducive to the recovery of vaginal flora balance in the later stage
[0007] Based on the above, the research on probiotic products for gynecological vaginal diseases in the prior art mainly focuses on the development and application of vaginal lactic acid bacteria, and there are few researches on compound probiotics

Method used

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  • Probiotic composition and use thereof
  • Probiotic composition and use thereof
  • Probiotic composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 probiotic composition capsule of the present invention

[0040] ① Freeze-drying of probiotics: After secondary activation of Lactobacillus acidophilus, Enterococcus faecalis and Bacillus cereus, the probiotic powder is made into freeze-dried powder, and the live bacteria content of the obtained bacteria powder is respectively: acidophilus Lactobacillus powder live bacteria count is 10 11 cfu / g, the viable count of Enterococcus faecalis is 10 10 cfu / g, the viable count of Bacillus cereus is 10 9 cfu / g.

[0041] ②Capsule preparation: mix Lactobacillus acidophilus, Enterococcus faecalis, and Bacillus cereus bacteria powder with microcrystalline cellulose, medicinal starch, and sodium carboxymethyl starch according to the calculated amount to prepare a pharmaceutical composition capsule, wherein:

[0042] High Dose Capsules: 1x10 Lactobacillus Acidophilus Viable Count 7 cfu / g, the viable count of Enterococcus faecalis is 1x10 7 cfu / g, t...

Embodiment 2 Embodiment 1

[0046] The in vitro antibacterial experiment of the probiotic composition capsule of embodiment 2 embodiment 1

[0047] 2.1 Observation of antibacterial effect by Oxford cup method

[0048] 2.1.1 Take 1 g of the freeze-dried bacterial powder in Example 1 and place it in 100 mL of seed liquid for activation culture, wherein, the preparation method of Lactobacillus acidophilus seed liquid is as follows: weigh 10 g of casein peptone, 10 g of beef extract, yeast Extract 5g, glucose 20g, dipotassium hydrogen phosphate trihydrate 5g, triammonium citrate 2g, sodium acetate 10g, magnesium sulfate 0.2g, manganese sulfate 0.05g, polysorbic acid 1g, add 1L purified water to dissolve, adjust pH to 5.8;

[0049] The formula of Enterococcus faecalis seed liquid is: weigh 10g of peptone, 5g of yeast extract, 5g of beef extract, 5g of glucose, 5g of sodium chloride, and 1g of polysorbate, add 1L of purified water to dissolve, and adjust the pH to 7.4;

[0050] The formula of the Bacillu...

Embodiment 3 Embodiment 1

[0064] The animal experiment of the probiotic composition capsule of embodiment 3 embodiment 1

[0065] Based on Example 1, the composite probiotic capsules of the present invention that are used to prevent or treat gynecological inflammation, each capsule weighs 0.5g, and each capsule powder is about 0.48g, and 10g of high-dose capsule powder is dissolved in 10mL of water, and the obtained high The number of live bacteria in the dosage sample solution is about 1x10 7 cfu / mL; Dissolve 10g of medium-dose capsule powder in 10mL of water, and the content of viable bacteria in the medium-dose sample solution is about 1x10 6 cfu / mL; Take 10g of low-dose capsule powder and dissolve it in 10mL water, and the content of viable bacteria in the medium-dose sample solution is about 1x10 5 cfu / mL. The number of live bacteria in each dose was tested before the experiment.

[0066] 3.1 Mucosa irritation test

[0067] Take 24 healthy and mature New Zealand female white rabbits with a m...

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Abstract

The invention provides a probiotic composition. The composition comprises lactobacillus acidophilus, enterococcus faecalis and bacillus cereus. The invention also provides a drug used for treating a vaginal disease. The drug contains the probiotic composition. The invention also provides the application of the probiotic composition in preparing the drug used for preventing and / or treating the vaginal disease. According to the application of the probiotic composition, the vaginal disease is gynecological vaginal inflammation. The probiotic composition has the advantages that the inhibition rateagainst escherichia coli is 86.5%, and the inhibition rate against staphylococcus aureus and candida albicans is 100%; animal experiments also verify that the probiotic composition does not have a stimulating effect on the vaginal mucosa.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a probiotic composition and its use in the preparation of products for preventing and / or treating gynecological vaginal diseases. More specifically, the present invention relates to a probiotic composition comprising Lactobacillus acidophilus, Enterococcus faecalis and Bacillus cereus and its use in the preparation of products for preventing and / or treating gynecological inflammation. Background technique [0002] The female lower reproductive tract is an important micro-ecological area of ​​the human body. In the vaginal discharge of healthy women, the ratio of anaerobic bacteria to aerobic bacteria is 5:1, and the number of viable bacteria is 10. 2 ~10 9 cfu / mL, the total bacterial count can reach 8×10 7 cfu / mL. There are more than 200 kinds of microorganisms parasitic in women's vagina, which grow in special forms on the surface of normal vaginal mucosa. In normal vaginal flora, La...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/747A61P15/02A61P31/04A61P31/10A61K35/744A61K35/742
CPCA61K9/0034A61K35/742A61K35/744A61K35/747A61P15/02A61P31/04A61P31/10A61K2300/00Y02A50/30
Inventor 闫天文沈玉保何炯灵冯增辉
Owner HANGZHOU GRAND BIOLOGIC PHARMA INC
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