Novel copper-doped-carbon-nano-dot-based method for enzyme-ratio-free fluorescence detection of organophosphorus pesticide

A technology of organophosphorus pesticides and carbon nano-dots, which is applied in the fields of nanomaterials, catalysis and chemical sensing, can solve the problems of preparation and application that have not been reported yet, and achieve the effects of good selectivity, simple operation method and good reproducibility

Active Publication Date: 2019-08-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the preparation and application of an enzyme-free ratiometric organophosphorus pesticide fluorescent se...

Method used

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  • Novel copper-doped-carbon-nano-dot-based method for enzyme-ratio-free fluorescence detection of organophosphorus pesticide
  • Novel copper-doped-carbon-nano-dot-based method for enzyme-ratio-free fluorescence detection of organophosphorus pesticide
  • Novel copper-doped-carbon-nano-dot-based method for enzyme-ratio-free fluorescence detection of organophosphorus pesticide

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Preparation of copper-doped carbon nanodots:

[0029] 40 mg of anhydrous copper chloride, 180 mg of tris(hydroxymethyl)aminomethane and 10 mg of L-dopa were dissolved in 40 mL of ultrapure water, and then placed in a hydrothermal reactor at 180°C for 4 hours. The above reaction solution was centrifuged at 12000 r / min for 10 min and filtered with a 0.22 μm microporous membrane to remove large-sized particles generated during the reaction, and finally freeze-dried and dispersed in ultrapure water for later use.

Embodiment 2

[0031] Morphology and performance analysis of copper-doped carbon nanodots:

[0032] figure 1 The transmission electron microscope image of the synthesized copper-doped carbon nanodots shows that the synthesized copper-doped carbon nanodots have an approximately spherical structure, the particle size distribution is between 20 and 40 nm, and the average size is 25 nm. Monodispersity and stability, it emits strong blue fluorescence under 365 nm UV lamp.

[0033] figure 2 X-ray photoelectron spectroscopy (XPS) of the as-synthesized copper-doped carbon nanodots. It can be seen from XPS that copper-doped carbon nanodots contain four elements, C, N, O and Cu, and their contents are 55.06%, 10.38%, 33.51% and 1.05%, respectively. It can be seen from the Cu2pXPS spectrum that two strong peaks appear at 932.5 ev and 952.5 ev, corresponding to Cu(0) and Cu(I), respectively. Therefore, the present invention successfully synthesizes copper-doped carbon nano-dots, and the valence sta...

Embodiment 3

[0043] Taking the detection of methapon as an example, the process of detecting organophosphorus pesticides in the present invention is illustrated: take 50 μL of copper-doped carbon nano-dots (10mg L -1 ), add 100 μL of different concentrations (0 ~ 500 μg·L -1 ) methapon solution, then add 100 μL hydrogen peroxide (50mmol·L -1 ) and 100 μL freshly prepared o-phenylenediamine (5 g L -1 ), dilute to 1 mL with ultrapure water, mix well and react at room temperature (25 °C) for 20 minutes, then place it on a fluorometer for fluorescence testing, and record the presence of different concentrations of killing agents in the system when the excitation wavelength is 330 nm. Phosphorus fluorescence spectrum ( Figure 6 ), record the fluorescence intensity ratio I of the reaction system at 375 nm and 555 nm 375 / I 555 ,Such as Figure 7 Shown, the fluorescence intensity ratio I 375 / I 555 By plotting the concentration of methapion and performing linear fitting, the standard work...

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Abstract

The invention, which relates to the technical fields of nano material, catalysis and chemical sensing, provides a novel copper-doped-carbon-nano-dot-based method for enzyme-ratio-free fluorescence detection of the organophosphorus pesticide. With anhydrous copper chloride, tris(hydroxymethyl) aminomethane and L-dopa as raw materials, fluorescent copper-doped carbon nano-dots with high stability and peroxidase activity are synthesized by a one-step hydrothermal method. O-phenylenediamine oxidized by hydrogen peroxide is catalyzed based on peroxidase-like activity of the copper-doped carbon nano-dots and fluorescent oxidized molecule 2,3-diaminophenazine is genreated, so that two fluorescent emission peaks of the copper-doped carbon nano-dot and 2,3-diaminophenazine are generated at the lengthes of 375 nm and 555 nm; and the ratio of the fluorescence intensities at the two wavelengths is linearly related to the concentration of the organophosphorus pesticide, thereby realizing enzyme-ratio-free fluorescence detection of the organophosphorus pesticide. The sensor is constructed simply and easily; no biological enzyme is needed; the influences of the probe concentration and environmental factor are low; and the linear range is wide and the sensitivity is high.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials, catalysis and chemical sensing, in particular to a method for detecting organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides are currently the most produced and used pesticides in my country. The residues of organophosphorus pesticides in agricultural products, water and soil directly lead to food poisoning and environmental pollution. Strengthening the monitoring of pesticide residues in agricultural products and environmental samples has important theoretical and practical significance for ensuring food safety and protecting the ecological environment. [0003] Chromatographic techniques such as gas chromatography, high performance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry have been widely used in the determination of organophosphorus pesticides. High, not suitable for on-site rapid detection...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 赵艳芳徐鲁斌常超
Owner QINGDAO AGRI UNIV
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