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A method for enriching Thiobacillus ferrooxidans with a hollow fiber membrane to form a biofilm, a hollow fiber membrane reactor and its application

A technology of Thiobacillus ferrooxidans and fiber membrane reactor, applied in the direction of microorganism-based method, method of supporting/immobilizing microorganism, bioreactor/fermenter combination, etc., can solve the problem that the culture medium cannot be reused, and achieve Reduce operating requirements and management costs, simple operation, and solve the effect that cannot be recycled

Active Publication Date: 2020-11-17
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a method for enriching Thiobacillus ferrooxidans with hollow fibers to form a biofilm, which realizes the separation of cultivation and utilization of Thiobacillus ferrooxidans, and solves the problem that the culture medium cannot The problem of reuse

Method used

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  • A method for enriching Thiobacillus ferrooxidans with a hollow fiber membrane to form a biofilm, a hollow fiber membrane reactor and its application
  • A method for enriching Thiobacillus ferrooxidans with a hollow fiber membrane to form a biofilm, a hollow fiber membrane reactor and its application
  • A method for enriching Thiobacillus ferrooxidans with a hollow fiber membrane to form a biofilm, a hollow fiber membrane reactor and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Enrichment and biofilm formation of Thiobacillus ferrooxidans:

[0047] Attached figure 1 Construct the reaction system with the hollow fiber membrane reactor as the core, then add 450mL liquid culture medium (the composition is: (NH 4 ) 2 SO 4 ,132;K 2 HPO 4 ,41; MgSO 4 ·7H 2 O,490; CaCl 2 2H 2 O,9; KCl,52; ZnSO 4 ·7H 2 O,1; CuSO 4 ·5H 2 O,2; MnSO 4 ·H 2 O,1; FeSO 4 ·7H 2 O,70; NaMoO 4 ·5H 2 O,0.5; CoCl 2 ·6H 2 O, 0.5; Na 2 SeO 4 10H 2 O,1; NiCl 2 ·6H 2 O, 1, the balance is water (unit: mg / L, the pH of the medium is adjusted to 2.0 with 5mol / L sulfuric acid)) and 50mL of acidic mine wastewater, turn on the culture medium circulation pump, and carry out enrichment culture and biofilm formation Open the valve of the high-pressure gas cylinder, adjust the pressure reducing valve to control the inlet pressure to be 0.06Mpa, supply the mixed gas of hydrogen and carbon dioxide (the volume ratio of hydrogen and carbon dioxide in the mixed gas is 4 : ...

Embodiment 2

[0050] Generate ferric sulfate leach solution that can be used for bioleaching, including intermittent flow mode (high concentration ferric sulfate leach solution) and continuous flow mode (low concentration ferric sulfate leach solution):

[0051] (1) Concrete steps under the discontinuous flow mode: the hollow fiber membrane reactor attached to the biofilm is pressed figure 2 Build a new reaction system; add 500mL ferrous sulfate solution (Fe in the solution) by the sampling port of the reaction system 2+ Concentration is 9g / L, and adjusts pH=2.0 with the sulfuric acid of 5mol / L), then open culture fluid circulation pump and water valve 2 (water valve 1 and water valve 3 are closed), start ferrous ion oxidation; Open air pump (air The air inlet of the pump is provided with a filter membrane to prevent dust from entering the air pump), adjust the pressure reducing valve to control the air inlet pressure, and supply air through the hollow fiber membrane reactor in a way of ai...

Embodiment 3

[0057] Generate schizite:

[0058] (1) The hollow fiber membrane reactor attached to the biofilm image 3 Build a new reaction system; add 500mL of ferrous sulfate solution (solution Fe 2+ The concentration is 9g / L, and the sulfuric acid of 5mol / L is used to adjust the pH=3.0), then turn on the circulation pump of the culture solution to start the oxidation of ferrous ions; turn on the air pump to feed air into the hollow fiber membrane, and adjust the pressure reducing valve to make the hollow fiber membrane No bubbles out; then turn on the water bath circulation system, and stabilize the temperature of the hollow fiber membrane reactor at 25-35°C by means of water bath heating; after 48 hours of reaction, turn off the culture medium circulation pump, and separate the minerals at the bottom of the reactor through the sampling port; minerals After cleaning and drying, the Shi's mineral is obtained, and the appearance of the Shi's mineral is as attached Image 6 shown.

[00...

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Abstract

The invention discloses a method for forming a biological membrane by enriching thiobacillus ferrooxidans by using a hollow fiber membrane, a hollow fiber membrane reactor and application. The methodincludes the steps that a reaction system with the hollow fiber membrane reactor as a core is built, a culture medium and an inoculation body are added, and thiobacillus ferrooxidans enrichment culture is carried out to form the biological membrane; mixed gas containing hydrogen is supplied to the surface of the membrane by the hollow fiber membrane reactor through bubble-free gas output; the temperature of the hollow fiber membrane reactor is stabilized at 25-35 DEG C through water bath heating; after several days of operation, a culture solution is discharged through a sampling port, and thehollow fiber membrane reactor with the membrane surface enriched with the thiobacillus ferrooxidans biological membrane is obtained; the hollow fiber membrane reactor enriched with the biological membrane can be used for generating an iron sulfate solution and schwertmannite. The method realizes separation of thiobacillus ferrooxidans culture and utilization, can achieve culture / utilization circulation, and solves the problem that the culture solution cannot be repeatedly used.

Description

technical field [0001] The invention relates to the field of microorganism cultivation and application, in particular to a method for enriching Thiobacillus ferrooxidans with a hollow fiber membrane to form a biofilm, a hollow fiber membrane reactor and its application. Background technique [0002] Realizing the efficient recovery of valuable metal resources in solid waste is one of the important ways to realize circular economy and solve global problems such as the depletion of mineral resources. Using Acithiobacillus ferrooxidans (A.f for short) to leaching valuable metals from solid waste has the characteristics of simple process control and environmental protection, and is a research hotspot in the field of hydrometallurgy. However, during the utilization process, the ferric ions, sulfate ions and typical monovalent cations (mainly: potassium ions, sodium ions and ammonium ions) in the leachate are very easy to form jarosite, Schwartz minerals, etc. Ferric alumite slag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C21B15/00C12M1/00C12R1/01
CPCC12M25/02C12N1/20C21B15/00
Inventor 杨远坤谌书王彬周日宇
Owner SOUTHWEAT UNIV OF SCI & TECH
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