Medicine carrier system with targeted medicine delivery capability in cancer cells and preparation method thereof

A technology of targeted drug delivery and carrier system, which is applied in the field of drug carrier system with the ability of targeted drug delivery in cancer cells and its preparation, which can solve the imbalance between oxygen supply and oxygen consumption, hypoxia, and reduce the effective oxygen concentration in PDT and other problems, to achieve the effect of alleviating hypoxia, enhancing absorption, and good biocompatibility

Active Publication Date: 2019-08-23
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, aberrant angiogenesis and poor blood flow within solid tumors can lead to an imbalance between oxygen supply and consumption, resulting in high levels of hypoxia
The hypoxic environment of tumor tissue may reduce the concentration of available oxygen in PDT and limit the generation of ROS

Method used

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  • Medicine carrier system with targeted medicine delivery capability in cancer cells and preparation method thereof
  • Medicine carrier system with targeted medicine delivery capability in cancer cells and preparation method thereof
  • Medicine carrier system with targeted medicine delivery capability in cancer cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A preparation method of a composite nano drug carrier system, specifically comprising the following steps:

[0066] 1. Synthesis of PB-CD-NH 2

[0067] Weigh 200 mg of PB and disperse in 100 ml of PBS with a pH of 5.5, then add EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 2.87 g and N-hydroxysuccinimide 1.7262g, reacted for 24 hours; then added 140mgEDA-β-CD and 60mg cystamine dihydrochloride to react for 24 hours, centrifuged and washed the obtained solid product, which was PB-CD-NH 2 .

[0068] 2. Synthesis of PB-C-dots-CD

[0069] Weigh 60mg of carbon quantum dots and disperse them in 100ml of PBS with a pH of 5.5, then add 1.7262g of EDC and 2.87g of N-hydroxysuccinimide in an ice-water bath at 4°C and react for 24 hours, then add 200mg of PB-CD-NH2 React at room temperature for 24 hours, and centrifuge to wash the obtained solid product, which is PB-C-dots-CD.

[0070] 3. Synthetic PLL (NF)

[0071] Weigh 200mg of PLL, 100mg of potassium c...

Embodiment 2

[0089] A preparation method of a composite nano drug carrier system, specifically comprising the following steps:

[0090] 1. Synthesis of PB-CD-NH 2

[0091] Weigh 200mg of PB and disperse it in 100ml of deionized water, adjust the pH to 5-6 with hydrochloric acid (if the adjustment is lower than 5, you can add a small amount of sodium hydroxide to adjust it), then add EDC (1-( 3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 2.87g and N-hydroxysuccinimide 1.7262g, reacted for 24 hours; then added 140mgEDA-β-CD and 60mg cystamine disalt The acid salt was reacted for 24 hours, and the obtained solid product was centrifuged and washed, which was PB-CD-NH2.

[0092] 2. Synthesis of PB-C-dots-CD

[0093] Weigh 60 mg of carbon quantum dots and disperse them in water, adjust the pH to 5-6 with hydrochloric acid, then add 1.7262 g of EDC and 2.87 g of N-hydroxysuccinimide in an ice-water bath at 4°C and react for 24 hours, then add 200 mg of PB-CD -NH2 was reacted at roo...

Embodiment 3

[0101] A preparation method of a composite nano drug carrier system, specifically comprising the following steps:

[0102] 1. Synthesis of PB-CD-NH 2

[0103] Weigh 200 mg of PB and disperse in 100 ml of PBS with a pH of 5.5, then add EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 2.87 g and N-hydroxysuccinimide 1.7262g, reacted for 24 hours; then added 180mgEDA-β-CD and 20mg cystamine dihydrochloride and reacted for 24 hours, centrifuged to wash the obtained solid product, which was PB-CD-NH 2 .

[0104] 2. Synthesis of PB-C-dots-CD

[0105] Weigh 60mg of carbon quantum dots and disperse them in 100ml of PBS with a pH of 5.5, then add 1.7262g of EDC and 2.87g of N-hydroxysuccinimide in an ice-water bath at 4°C and react for 24 hours, then add 200mg of PB-CD-NH2 React at room temperature for 24 hours, and centrifuge to wash the obtained solid product, which is PB-C-dots-CD.

[0106] 3. Synthetic PLL (NF)

[0107] Weigh 200 mg of PLL, 100 mg of potassium...

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Abstract

The invention discloses a medicine carrier system. Prussian blue modified by carbon quantum dots and beta-cyclodextrin is used as a substrate; a product is obtained through the combination with NO donor 5-chloro-2-nitrophenyl trifluoromethane grafted polylysine after the modification by folic acid. The medicine carrier system provided by the invention can realize the effective enriching in the tumor positions through folic acid targeting and tumor position EPR effect; under the near infrared laser irradiation, Prussian blue nanometer particles can melt tumor cells through high photothermal conversion efficiency. Meanwhile, the medicine carrier system can control the release of nitric oxide under the illumination condition of the wavelength being 400nm, so that the enrichment of the nanometer particles in the tumor positions through the EPR effect can be improved; meanwhile, NO can achieve the effect of inhibiting the tumor growth through inducing the tumor cell apoptosis, reversing multidrug resistance and the like.

Description

technical field [0001] The invention belongs to the field of drug carriers, and in particular relates to a drug carrier system capable of targeting drug delivery in cancer cells and a preparation method thereof. Background technique [0002] In recent years, with the deterioration of the natural environment, the incidence of cancer is increasing. Traditional cancer treatments include: surgery, chemotherapy and radiotherapy. Although it can prolong the life of patients to a certain extent, these treatment methods generally have the disadvantages of relatively large toxic and side effects, damage to normal tissues and large trauma, which limit their therapeutic effect on tumors. Therefore, the development of new therapeutic modalities has received increasing attention. [0003] Currently, chemotherapy is the main anti-tumor treatment, but it is challenged by multidrug resistance (MDR), which largely limits the efficacy of chemotherapy. And often use high doses and increase ...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K41/00A61K47/61A61K47/64A61K47/54A61P35/00
CPCA61K41/0042A61K41/0052A61K49/0067A61K47/545A61K47/61A61K47/645A61P35/00A61K2300/00
Inventor 李草陈重银万立辉陈辉段军林徐翔宇卢金博罗毕矗江兵兵
Owner HUBEI UNIV
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