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Artemisinin-loaded citrus pectin oral nanoparticle

A technology of nanoparticles and artemisinin, which is applied in the field of oral nanoparticles of citrus pectin, can solve the problems of good biocompatibility, instability, and no discovery.

Active Publication Date: 2019-09-06
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, concanavalin and glucose oxidase are proteins, which have the disadvantages of high cost, instability, and strong antigenicity.
[0007] Therefore, although the way of oral medicine is the best choice for diabetic patients, it has not yet found a drug that has a stable point, low toxicity, good biocompatibility, high drug loading rate and release rate, and has a certain effect on diabetic complications. Oral drugs

Method used

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  • Artemisinin-loaded citrus pectin oral nanoparticle
  • Artemisinin-loaded citrus pectin oral nanoparticle
  • Artemisinin-loaded citrus pectin oral nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] The preparation of the oral nano system of embodiment 1 loading artemisinin & citrus pectin

[0106] The preparation process of oral nano-particles loaded with artemisinin / citrus pectin is as follows: figure 1 Shown:

[0107] Step 1: Preparation of MCP(ART) nanoparticles

[0108] First denature the citrus pectin, place the citrus pectin in distilled water at a concentration of 1.5 mol / L, and increase its pH to 10.0 with sodium hydroxide (3 mol / L) at 55°C, and keep stirring for 1 hour , and then the solution was cooled to room temperature, its pH was adjusted to 10.0 with sodium hydroxide (3mol / L), and the denatured citrus pectin was dissolved.

[0109] Then add the artemisinin that has been dissolved in the organic solvent under the condition of 55°C, so that the final concentration of artemisinin is 1.5mol / L, stir at 600rmp for 2 hours, add calcium hydroxide to the final concentration of 0.01mol / L, Stir at 55°C and 600rmp for 1 hour, finally add sodium bicarbonate t...

Embodiment 2

[0115] Example 2 Characterization of Oral Nanoparticles Loaded with Artemisinin & Citrus Pectin

[0116] 1. Infrared spectrum detection

[0117] The raw materials MCP, AAPBA, TMC synthesized by the system and the synthetic materials MCP(ART), MCP(ART)-AAPBA and MCP(ART)-AAPBA-TMC prepared in Example 1 were detected by infrared spectroscopy.

[0118] 1. Experimental method

[0119] Freeze-dry the sample to be tested, then put it into a mortar, add a certain amount of KBr, and grind the mixture evenly until the particle size is less than 2 μm, so as to avoid the influence of scattered light, and then put it in a dryer for drying. Press the mixture into a transparent sheet with a pressure of about 10 MPa on a hydraulic press, and use a German Bruker VERTEX 33 Fourier transform infrared spectrometer to measure it.

[0120] 2. Experimental results

[0121] In order to understand the changes in the stage of forming loaded artemisinin / citrus pectin particles, the loaded artemisini...

Embodiment 3

[0165] Example 3 Cell Experiment of Oral Nanoparticles Loaded with Artemisinin & Citrus Pectin

[0166] 1. Experimental method

[0167] The biotoxicity of MCP(ART)-AAPBA-TMC oral nanotransport carrier at the cellular level will be evaluated by MTT method.

[0168] 1. Cell culture

[0169] The HepG-2 cell line was provided by Guangdong Pharmaceutical University. After the cells were proliferated and cultured to 80% in the culture flask, they were seeded on a 96-well plate at a density of 5000 / well, and cultured for 1 or 2 days for subsequent experiments. The cell culture conditions are: high-glucose DMEM medium containing 20% ​​newborn calf serum, non-essential amino acids, 37°C, 5.0% CO 2 .

[0170] 2. Toxicity of MTT transport system

[0171]Different doses of the transport system were added to the cells being cultured, and after 24 hours, the cell viability was measured by the MTT method. That is, inoculate HepG-2 cells on a 96-well culture plate, 5000 cells / well, afte...

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Abstract

The invention discloses an artemisinin-loaded citrus pectin oral nanoparticle; a preparation method of the oral nanoparticle includes the following steps: the citrus pectin is subjected to multimolecular self-polymerization; nanoparticles are formed from the citrus pectin and wrapped and adsorbed with theartemisinin; aminophenylboronic acid is connected with the citrus pectin nanoparticles; the outer layer is wrapped with denatured chitosan. For the first time, citrus pectin and artemisinin are combined to improve insulin resistance and pancreatic beta cell injury in type II diabetes at the same time; the method of transforming pancreatic alpha cells into pancreatic beta cells is applied to nano-therapy of type II diabetes, can inhibit the secretion of glucagon and also can improve insulinsecretion; no matter drugs nor delivery materials adopt natural products from plants to ensure no toxicity and easy metabolism in the process of treatment of type II diabetes.

Description

technical field [0001] The invention relates to the technical field of medical nanomaterials, in particular to an oral nanoparticle of citrus pectin loaded with artemisinin. Background technique [0002] Diabetes is a metabolic disease with multiple etiologies. It is manifested in the insufficient secretion of insulin by the pancreas or the decreased sensitivity of the surrounding tissues to insulin. Therefore, the liver, muscle and other tissues cannot convert glucose in the blood. The number of people suffering from diabetes in the world is about 280 million, of which 5% to 10% are type I diabetes, and type II diabetes accounts for 90% to 95% of new diabetes cases. 70% to 90% of the islet β cells have been lost at the first onset of type I diabetes, and all of the islet β cells die 1 to 2 years after the onset, and the insulin secretion is about zero. Type II diabetes usually occurs between the ages of 35 and 40. Patients even produce too much insulin in the body, the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/69A61K31/366A61K9/52A61K47/36A61P3/10A61P5/50
CPCA61K31/69A61K31/366A61K9/5161A61P3/10A61P5/50A61K2300/00Y02A50/30
Inventor 关燕清李健黄景敏
Owner SOUTH CHINA NORMAL UNIVERSITY
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