Lactobacillus fermentum KP101 and application thereof

A technology of Lactobacillus fermentum, KP101, applied in the directions of Lactobacillus, application, Streptococcus/Lactococcus, etc., can solve the problems of influence, reduce TC and LDL-C levels, etc., to improve HDL-C level, anti-pathogenic bacteria Good effect on infection, tolerance to bile salt stress

Active Publication Date: 2019-09-10
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that probiotics can significantly reduce serum TC and LDL-C levels in experimental animals, and no probiotics have been found to have a significant impact on serum HDL-C levels in experimental an

Method used

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  • Lactobacillus fermentum KP101 and application thereof
  • Lactobacillus fermentum KP101 and application thereof
  • Lactobacillus fermentum KP101 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening and identification of Lactobacillus fermentum KP101

[0028] 1. Screening of bacterial strains

[0029] The kimchi samples purchased in the market were isolated from lactic acid bacteria in the laboratory; -1 Gram-positive strains were selected by observing the colony morphology with Gram staining method; The selected strains were then cultured in liquid MRS medium at 37°C for 24 hours, inoculated according to the inoculation amount of 3% of the culture medium, and activated and passaged continuously for three times to ensure the vigorous growth of the bacteria;

[0030] Liquid MRS medium contains peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate [(NH4)2HC6H5O7] 2.0g, glucose (C6H12O6 H2O) 20.0g, Tween 80 1.0mL, sodium acetate (CH3COONa·3H2O) 5.0g, dipotassium hydrogen phosphate (K2HPO4·3H2O) 2.0g, magnesium sulfate (MgSO4·7H2O) 0.58g, manganese sulfate (MnSO4·H2O) 0.25g.

[0031] 2. Preparation of bacterial suspen...

Embodiment 2

[0050] Example 2 Probiotic tolerance test

[0051] 1. Evaluation of acid resistance

[0052] The strains were inoculated in liquid MRS medium according to the inoculum volume of 3%, and cultured at 37°C for 18h. culture medium at 4°C at 4000rpm·min -1 Centrifuge for 10 minutes to obtain the bacteria sludge, wash twice with 0.85% normal saline, and then suspend the bacteria sludge with sterile normal saline to ensure that the activated bacterial strain is about 10 8 CFU mL -1 , and then inoculate the bacterial suspension in the MRS medium with a pH of 2.0 and 3.0 according to the inoculation amount of 3%, take samples at 0h, 2h, and 4h respectively, dilute 10 times with normal saline, and spread it on the MRS after diluting the appropriate concentration. In the solid medium, culture at 37°C for 48 hours, count the colonies, make three parallels for each concentration, calculate the average value, and calculate the number of viable bacteria and survival rate;

[0053] Result...

Embodiment 3

[0067] Embodiment 3 antioxidant activity test

[0068] 1.H 2 0 2 tolerance test

[0069] Inoculate the pure culture of Lactobacillus fermentum KP101 into MRS broth supplemented with different concentrations of hydrogen peroxide (0.5, 1.0 mmol / L); measure the absorbance at 600nm every 2h from 0h, and calculate the survival rate;

[0070] Results: The tolerance of the strain to hydrogen peroxide was concentration-dependent. The strain had strong tolerance to low concentration of hydrogen peroxide (0.5mM), and the survival rate of 37°C for 4 hours increased significantly. (1mM) the survival rate increased less, and the survival rate of the 8h strain was not less than 90%, indicating that Lactobacillus fermentum KP101 has strong tolerance to different concentrations of hydrogen peroxide ( Image 6 a).

[0071] 2. Clear DPPH free radicals

[0072] Centrifuge the bacterial liquid at 4000r / min for 10min to obtain the fermentation supernatant and bacterial cells respectively, was...

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Abstract

The invention discloses a strain of lactobacillus fermentum KP101 and application thereof in preparing fermented yogurt and probiotic solid beverages. The preservation number of the lactobacillus fermentum KP101 is CCTCC No. 2019085. The lactobacillus fermentum KP101 has good acid stress resistance, cholate stress resistance and oxidative stress resistance, can resist pathogenic infection, regulate metabolic disturbance, regulate hyperlipidemia and the like, and also can reduce fat and reduce weight. Animal experiments show that the increase of TG and LDL-c in serum caused by high fat diet canbe effectively reduced by 50.5% and 67.0% respectively; the level of HDL-C in liver can be significantly improved by 78.9%; the contents of TC, TG and LDL-C in liver can be reduced by 36.2%, 64.6% and 42.8% respectively; a significant fat reducing effect is also achieved, and the perirenal fat reduction coefficient and the abdominal fat reduction coefficient are 97.2% and 95.5% respectively.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a strain of Lactobacillus fermentum KP101 and its application. Background technique [0002] Hyperlipidemia and its complications have become one of the diseases with the highest morbidity and mortality in the world, but the current treatment methods are still very limited. There are two main clinical treatment methods, one is dietary intervention, and the other is drug treatment. The core of dietary intervention is to limit the intake of high-fat and high-cholesterol foods. However, this method is only effective in a short period of time and is difficult to persist for a long time, and its therapeutic effect gradually deteriorates; although drug treatment is relatively obvious, cholesterol-lowering drugs often have large side effects and are expensive. As a class of substances with low price and high safety, probiotics can regulate and control serum c...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23L2/39A23L2/52A23L33/135C12R1/225
CPCA23C9/1238A23C9/1234A23L2/39A23L2/52A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/143A23V2400/249A23V2200/30
Inventor 王玉华滕跃王宇王朝孙海月
Owner JILIN AGRICULTURAL UNIV
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