PEG-modified magnetic/quaternized chitosan nanoparticles with peroxidase activity, and preparation method and application thereof

A technology of quaternized chitosan and magnetic nanoparticles, which can be applied in non-active medical preparations, pharmaceutical formulations, inorganic non-active ingredients, etc., and can solve the problem of low interaction rate, low bacterial removal efficiency, heavy metal leakage, etc. problem, to achieve the effect of reducing H2O2 concentration, good biodegradability, and increasing antibacterial efficiency

Active Publication Date: 2019-09-17
天津市泌尿外科研究所
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, in practical applications, such as in biological sciences and medical sciences, the further application of reported magnetic nanoparticles is still limited by the following: (1) Many magnetic nanoparticles lack good biocompatibility, especially those compounded with Au The application of metal nanoparticles such as Ag and Ag in the fields of bioscience and medicine may lead to the leakage and accumulation of heavy metals in the environment (soil, water, human body, etc.); (2) it is difficult to eradicate bacteria with ultra-low concentrations of bacteria; (3) use biomolecular functions The optimized magnetic

Method used

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  • PEG-modified magnetic/quaternized chitosan nanoparticles with peroxidase activity, and preparation method and application thereof
  • PEG-modified magnetic/quaternized chitosan nanoparticles with peroxidase activity, and preparation method and application thereof
  • PEG-modified magnetic/quaternized chitosan nanoparticles with peroxidase activity, and preparation method and application thereof

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Embodiment 1

[0036] Embodiment 1: High magnetic nanoparticle Fe 3 o 4 @PEG / PAA / CS-N(CH 3 ) 3 + preparation of

[0037] 1) Preparation of highly magnetic ferric iron tetroxide Fe rich in carboxyl groups 3 o 4 Nanocrystalline particles; ferric chloride hydrate (6.5g), trisodium citrate dihydrate (1.8g) and sodium acetate (7.2g) were dissolved in ethylene glycol (120ml). The resulting mixture was sealed in a Teflon-lined stainless steel autoclave, heated to 200°C for 10 hours, and then cooled to room temperature. The product was separated magnetically, washed thoroughly with ethanol and deionized water, and finally dried under vacuum at 50 °C to constant weight.

[0038] 2) Preparation of PEGylated magnetic chitosan particles Fe3O4@PEG / PAA / CS; 2.1) Preparation of CS / AA filtrate: Weigh 220mg AA, 22mg MBA and 500mg refined CS (molecular weight about 40,000) in sequence, add 20mL double distilled water, stirred overnight at room temperature to dissolve CS and fully electrostatically comp...

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Abstract

The invention belongs to the field of composite material, and particularly relates to PEG-modified magnetic/quaternized chitosan magnetic nanoparticles with peroxidase activity, and a preparation method and application thereof. The PEG-modified magnetic/quaternized chitosan magnetic nanoparticles with peroxidase activity comprise highly magnetic Fe3O4 as a magnetic core, a crosslinked polyamide/chitosan polyelectrolyte complex in the middle layer, and a quaternized chitosan and hydrophilic polyethylene glycol chain as the outer shell. The intermediate polymer layer adequately protects the high magnetic property and peroxidase activity of the Fe3O4, and the quaternized chitosan and hydrophilic polyethylene glycol chain as the shell provides long-term water stability and dispersibility for the nanoparticles. Nanoparticles have a fast and efficient "encirclement and suppression" effect on bacterial pathogens. The strategy uses a bacterial capture nanocomposite containing the magnetic nanoparticles with peroxidase-like activity under a trace amount of H2O2.

Description

technical field [0001] The invention belongs to the field of composite materials, and in particular relates to a magnetic / quaternized chitosan magnetic nanoparticle modified with peroxidase activity and a preparation method and application thereof. Background technique [0002] Bacterial infectious diseases can significantly affect human health and result in significant morbidity, mortality, and high healthcare costs. Traditional antibiotic therapy, which has saved millions of lives for decades, remains the treatment of choice for bacterial infections. However, in addition to the disadvantages of high cost and potential health and ecosystem risks, the widespread overuse of antibiotics can lead to multidrug-resistant strains, leading to bacterial infectious diseases that are no longer curable with existing drugs. Currently, thanks to the development of nanotechnology, various nanomaterials play an important role in the fight against bacterial infections, such as metal nanost...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/36A61K47/10A61K47/34A61K47/02A61P31/04
CPCA61K9/5161A61K9/5146A61K9/5115A61P31/04Y02A50/30
Inventor 王晓娟傅国旗顾汉卿
Owner 天津市泌尿外科研究所
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