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The application of Pleurotus pellagra to the production of ergothioneine

A technology of Pleurotus pellagra and ergothioneine, applied in the direction of microorganism-based methods, fungi, microorganisms, etc., can solve the problems of long fermentation time and slow growth, achieve high yield, reduce energy consumption, and reduce production and preparation costs Effect

Active Publication Date: 2020-06-26
弘恒泰(天津)科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although a variety of fungal and mycobacterial sources are available for ergothioneine extraction, current research has focused on the production of ergothioneine by macrofungal species such as Pleurotus pleurotus, Pleurotus pulmonica, wild Pleurotus pleurotus, Pleurotus rugosa and monkey Head mushrooms, etc., but most of the fungi are suitable for low temperature growth, and the growth is slow and the fermentation time is long

Method used

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  • The application of Pleurotus pellagra to the production of ergothioneine
  • The application of Pleurotus pellagra to the production of ergothioneine
  • The application of Pleurotus pellagra to the production of ergothioneine

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preparation example Construction

[0060] The preparation method of the PDA slant medium is as follows: take 200g peeled potatoes, cut into small pieces and put them in a pot, add 980ml of water, heat to boiling on a heater, keep for 20min, filter on a measuring cup while hot with 2 layers of gauze, Obtain the filtrate, discard the filter residue, add 20g of glucose and 25g of agar to the filtrate, heat and melt, and set the volume to 1000mL, the pH value is natural, and distribute it in test tubes or eggplant bottles with the corresponding 7mL / test tube or 70mL / eggplant bottle. Put on the rubber stopper, sterilize under high temperature and high pressure at 121±1°C for 30 minutes, and spread the inclined surface for later use.

[0061] RSL-HM2 strain grows faster on PDA medium (potato dextrose agar medium). One piece of mycelium grows for 10 days in the dark at 32°C. Lush and light brown on the back, its colony shape is as follows figure 1 shown. Under the microscope, the hyphae are densely clustered in the ...

Embodiment 1

[0069] When the solid substrate is the basic fermentation medium, the basic fermentation medium is 45g maltodextrin, 20g casein peptone, 4g potassium dihydrogen phosphate, 2g magnesium sulfate heptahydrate, 1g ammonium dihydrogen phosphate, 1g cysteine, 1g A mixture of methionine and vitamin B1 1mg.

Embodiment 2～13

[0071] When the solid substrate is a carbon source optimized medium, the carbon source optimized medium is 45g carbon source, 20g casein peptone, 4g potassium dihydrogen phosphate, 2g magnesium sulfate heptahydrate, 1g ammonium dihydrogen phosphate, 1g cysteine, A mixture of 1g of methionine and 1mg of vitamin B1, the carbon sources are: glycerin, glucose, fructose, galactose, mannitol, sorbitol, lactose, sucrose, maltose, maltodextrin, dextrin, starch or corn flour, see the table for details 3.

[0072] table 3

[0073] Example carbon source Example 1 glycerin Example 2 glucose Example 3 fructose Example 4 Galactose Example 5 Mannitol Example 6 Sorbitol Example 7 lactose Example 8 sucrose Example 9 maltose Example 10 Maltodextrin Example 11 dextrin Example 12 starch Example 13 corn flour

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Abstract

The invention discloses the application of a high-temperature-resistant Pleurotus ergotitis in the production of ergothioneine. The high-temperature-resistant Pleurotus urticaria was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on October 18, 2018, and the preservation number is For CGMCC:16378. The application of the high-temperature-resistant Pleurotus ergothioneine in the production of ergothioneine has the following advantages: 1, the high-temperature-resistant Pleurotus ergothioneine shortens the fermentation time of ergothioneine; 2, the cultivation temperature of the ergothioneine fermentation production process can reach up to 32 ℃, can reduce the energy consumption brought by the cultivation and cooling, and reduce the production cost; 3. The yield of ergothioneine is higher, which reduces the production and preparation cost of ergothioneine, and lays a solid foundation for the industrial production and commercial application of ergothioneine The basics.

Description

technical field [0001] The invention belongs to the technical field of high-temperature-resistant Pleurotus rugosa, and specifically relates to the application of a high-temperature-resistant Pleurotus rugosa in the production of ergothioneine. Background technique [0002] Ergothioneine is a natural and rare chiral amino acid with strong antioxidant function. Compared with other antioxidants, its physical, chemical and biochemical properties are more stable. Ergothioneine has the functions of scavenging free radicals, maintaining DNA biosynthesis, detoxification, anti-radiation, cellular immunity and energy regulation in organisms. It can be expected that ergothioneine has important application value in cosmetics, food and biomedicine industries . [0003] Ergothioneine is a natural compound produced in actinomycetes such as Mycobacterium smegmatis and filamentous fungi such as Neurospora crassa, and recently the fission yeast Schizosaccharomyces pombe was also found to be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P13/04C12R1/645
CPCC12P13/04C12N1/145C12R2001/645
Inventor 孟昭晖
Owner 弘恒泰(天津)科技发展有限公司
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