A thermosensitive hydrogel modified by calcium magnesium silicate nano hollow microspheres and its preparation method and application

Abstract

The invention provides calcium silicate nano hollow microsphere modified thermosensitive hydrogel and a preparation method and application thereof. The preparation method includes: preparing calcium silicate nano hollow microspheres, dispersing the microspheres in the complex solution of carboxymethyl chitosan and beta-sodium glycerophosphate, and processing at 30-70 DEG C. The preparation methodhas the advantages that the method is simple and low in cost, the reaction conditions of the method can be achieved easily, and the raw materials used by the method are cheap and easy to obtain; the prepared thermosensitive hydrogel is safe, low in cytotoxicity, good in biocompatibility and good in low-temperature flowability, the hydrogel can fast turn into gel at 37 DEG C, the surface of the gelis loose and porous, and the gel is large in specific surface and capable of benefiting neural stem cell adsorption; the calcium silicate nano hollow microspheres are evenly dispersed in the thermosensitive hydrogel, and the thermosensitive hydrogel can promote neural stem cell differentiation; when the thermosensitive hydrogel is applied to brain tissue damage repairing, neural stem cell adsorption can be promoted effectively, the neural stem cells can be effectively promoted to differentiate into neurons and neuroglia, and the thermosensitive hydrogel is promising in clinical application prospect.

Description

technical field [0001] The invention relates to a temperature-sensitive hydrogel modified by calcium magnesium silicate nano hollow microspheres and a preparation method and application thereof, belonging to the technical field of biomaterials. Background technique [0002] Brain injury is an extremely serious type of nervous system injury, characterized by high mortality, high disability rate, high treatment costs, and long recovery time, which seriously threatens human life, health and living standards, and brings great harm to society and families heavy burden. At present, there is no simple and effective treatment for brain tissue damage. In most cases, it can only rely on the patient's cell growth ability to repair itself. The relatively new allogeneic neural stem cell repair technology also has the problem that the neural stem cells injected into the affected area cannot stably exist in the affected area and cause damage. The problem of directed differentiation. [0...

AI Technical Summary

Problems solved by technology

However, the hydrogel in this invention is prepared through free radical polymerization, the preparation process needs to be fully cleaned, the amount of waste water is large, and the final hydrogel inevitably contains harmful substances such as organic solvents and unreacted monomers. High toxicity; the obtained hydrogel has poor biocompatibility, and biocompatibility is an important criterion for judging whether a certain material can be used in biomedicine; and it does not have temperature sensitivity, low specific surface area, and poor adsorption capacity Good, not conducive to subsequent applications

[0005] In addition, the existing hydrogels are mostly used as scaffold materials, and the functions that can be given are limited

For example, the promotion of nerve repair is mostly based on the adsorption and release of growth factors by hydrogel materials, thereby affecting nerve differentiation; there are few scaffold materials that can promote nerve differentiation prepared by inorganic-organic composite materials

Moreover, there is no morphological change before and after the application of traditional hydrogels, which requires that in the surgical repair, a specific shape should be prepared in advance for filling, and it will cause a large wound area, which is not conducive to tissue repair and sufficient and tight filling of hydrogels.

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