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Bifidobacterium adolescentis CCFM1061, fermented food thereof and preparation method of microbial agent

A technology of bifidobacteria and fermented food, applied in bifidobacteria, bacteria used in food preparation, biochemical equipment and methods, etc., can solve the increase of drug toxicity and side effects, liver function damage and edema, adverse reactions of digestive tract, etc. It can reduce the absorption, alleviate the toxicity of PFOA, and improve the antioxidant capacity.

Active Publication Date: 2019-10-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have a certain therapeutic effect, but as the disease worsens, the dosage increases, drug interactions, and drug side effects also increase significantly, leading to adverse reactions in the digestive tract and showing certain liver and kidney toxicity, such as Long-term use of metformin can cause discomfort in the gastrointestinal tract of some patients and may affect the absorption of vitamin B12. Rosiglitazone can cause liver damage and edema, etc.

Method used

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  • Bifidobacterium adolescentis CCFM1061, fermented food thereof and preparation method of microbial agent
  • Bifidobacterium adolescentis CCFM1061, fermented food thereof and preparation method of microbial agent
  • Bifidobacterium adolescentis CCFM1061, fermented food thereof and preparation method of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Characteristics of Bifidobacterium adolescentis CCFM1061:

[0046] (1) Bacterial characteristics: Gram-positive bacteria that do not form spores and do not move;

[0047] (2) Colony characteristics: After 36 hours of anaerobic culture, obvious colonies are formed, with a diameter between 0.5-1mm, a round front shape, a protruding side shape, neat edges, milky white, translucent, moist and smooth surface, and no pigmentation , see attached figure 1 ;

[0048] (3) Growth characteristics: under the condition of constant temperature and anaerobic at 37° C., cultivate in the modified mMRS medium for about 20 hours to reach the end of logarithmic phase.

[0049] (4) Significantly increase the abundance of Akkermansia in the intestinal tract of NAFLD mice, and reduce the occurrence of diseases such as obesity, diabetic fatty liver and enteritis;

[0050] (5) Significantly improve lipid metabolism disorder in NAFLD mice;

[0051] (6) Significantly improved insulin resistanc...

Embodiment 2

[0099] Example 2: Regulatory effect of Bifidobacterium adolescentis CCFM1061 on intestinal flora of NAFLD mice

[0100] Take 48 healthy male C57BL / 6J mice weighing 16-20g, adapt to the environment for 1 week, and randomly divide them into 6 groups: blank control group (NC), model control group (M), rosiglitazone control group (RC) , simvastatin control group (SC), Bifidobacterium adolescentis CCFM1061 intervention group (CCFM1061), Lactobacillus rhamnosus L10 intervention group (LC), each group contains 8 mice. The experimental animal groups and treatment methods are shown in Table 2:

[0101] Table 2 Grouping of experimental animals

[0102]

[0103]

[0104] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the metagenome in the feces was extracted, and the structure of the intestinal flora was analyzed using a next-generation sequencer. At the end of the experiment, the mice were fasted for 12 hours, anesthetized by intrap...

Embodiment 3

[0107] Embodiment 3: Bifidobacterium adolescentis CCFM1061 significantly reduces the level of serum total cholesterol (TC) in NAFLD mice

[0108] C57BL / 6J mice grouping, modeling and treatment methods were the same as in Example 2.

[0109] Experimental results such as image 3 shown. The serum total cholesterol content of the mice in the model group was significantly increased, and oral administration of Bifidobacterium adolescentis CCFM1061 significantly reduced the TC level of the model mice and was close to that of the blank control group. Its ability to reduce serum TC in mice was similar to that of the simvastatin drug group.

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Abstract

The invention discloses Bifidobacterium adolescentis CCFM1061, a fermented food thereof and a preparation method of a microbial agent. The Bifidobacterium adolescentis CCFM1061 can remarkably improveliver injury of patients with non-alcoholic fatty liver disease, reduce serum ALT level, and reduce liver adiposis, liver balloon-like lesion and liver lobular inflammation; can obviously relieve theincrease of contents of serum total cholesterol, blood glucose, liver triglyceride and low-density protein cholesterol caused by high-fat diet in patients with non-alcoholic fatty liver disease; meanwhile, can obviously improve the anti-oxidation capacity of the liver of a NAFLD patient; can obviously improve the expression of Nrf2 gene of fatty liver cells; and can improve the fasting blood glucose of a type II diabetic mouse. In addition, the Bifidobacterium adolescentis CCFM1061 has strong adsorption capability to perfluorooctanoic acid, reduces the absorption of the perfluorooctanoic acidin vivo, and has the capability of relieving PFOA toxicity.

Description

technical field [0001] The invention belongs to the technical field, and in particular relates to bifidobacterium adolescent CCFM1061, its fermented food and a preparation method of bacterial agent. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is fatty degeneration caused by excessive accumulation of fat in liver cells. It is a symptom of liver metabolic disorders. Without intervention, it can gradually evolve into non-alcoholic fat Hepatitis (NASH) further deteriorates into liver fibrosis, liver cirrhosis, and liver cancer with extremely high mortality rates. In recent years, with the improvement of living standards, more and more high-fat and high-energy diets are put on the table, but people's exercise has not increased accordingly, which makes metabolic syndrome represented by non-alcoholic fatty liver disease The incidence of symptoms is increasing year by year. Due to its imperfect pathological mechanism, there are no drugs specifically tar...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/133A23C20/02A23L33/00C12R1/01
CPCC12N1/20A23C9/133A23C20/025A23L33/00C12R2001/01C12N1/205A23V2400/513
Inventor 陈卫王刚焦婷赵建新张灏
Owner JIANGNAN UNIV
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