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Streptomyces griseus HL-50, and complex microbial inoculant and application thereof

A technology of Streptomyces griseus and complex bacterial agents, applied in the field of microorganisms, can solve the problems of affecting the use effect of essential oils, strong but not long-lasting smell, not thick sense, etc., to inhibit the reproduction of harmful bacteria, improve growth, and repel insects good effect

Active Publication Date: 2019-11-19
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the essential oils, there is a kind of eucalyptus essential oil, its color is very light, the sense is not thick, and the smell is strong but not long-lasting, which will eventually affect the use effect of the essential oil

Method used

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  • Streptomyces griseus HL-50, and complex microbial inoculant and application thereof
  • Streptomyces griseus HL-50, and complex microbial inoculant and application thereof
  • Streptomyces griseus HL-50, and complex microbial inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Screening of Streptomyces griseus HL-50

[0034] Take the soil as a sample, mix the soil and sterile water to make 10% of the soil -1 diluent, and then prepare 10 -2 、10 -3 、10 -4 、10 -5 Diluent. Take 10 respectively -3 、10 -4 、10 -5 The diluted solution was spread on Gaoshi Synthetic No. 1 agar medium, cultured at 30°C for 7 days, and the obvious colonies were selected for multiple purifications to obtain a single colony, which was named HL-50, and placed on the slope of Gaoshi Synthetic No. 1 agar. stored in culture medium. The formula of described Gao's synthetic No. 1 agar medium is: KNO 3 1g, 20g soluble starch, K 2 HPO 4 0.5g, MgSO 4 ·7H 2O 0.5g, NaCl 0.5g, FeSO 4 0.01g, 20g agar, 1000mL distilled water, pH 7.2.

[0035] like figure 1 As shown, the biological characteristics of the colony of the bacterial strain HL-50 on Gaoshi Synthetic No. 1 agar medium are: the colony is round, with a small area, a slight protrusion in the middle, light kh...

Embodiment 2

[0040] 1, the preparation of eucalyptus eucalyptus essential oil

[0041] The preparation process of the Eucalyptus eucalyptus essential oil in the composite microbial agent described in the present invention is as follows figure 2 As shown, first put the leaves of Eucalyptus eucalyptus in Australia into the washing tower for cleaning, put them in the dryer for drying, and then pulverize them in the pulverizer and then enter the steam distillation kettle, condenser and oil-water stratifier for separation The oil phase and the water phase, the water phase enters the ether extractor to separate the waste water, the liquid containing ether enters the ether recovery tower to recover ether, and the recovered ether and oil phase liquid are added to the solid bed adsorption dryer to further remove the ether in the oil phase Finally, the oil phase liquid is added to a vacuum rectification tower for rectification to remove impurity oils to obtain eucalyptus multibract essential oil. ...

Embodiment 3

[0046] The compound bacterial agent prepared in Example 2 is carried out deworming test, and the test site is selected in Sheyang, Jiangsu, with a breeding area of ​​80 mu, and the breeding mode is shown in the following table:

[0047] farming model

[0048]

[0049] After the parasites were found in the gills of the fish, 1 kg of compound bacterial agent was used to mix 1 ton of feed, and it was carried out according to the normal feeding management, and the feeding was continued for 7 days, 3 times a day. After 7 days, check the gills of the fish again for parasites. like Figure 4 As shown, at the initial stage of feeding, the gills of crucian carp were infected with sporozoites, trichomonas and ringworms; after feeding for 7 days, the gills of crucian carp had no sporozoites infection, and there were no trichomonas and ringworms under the microscope. Fish has obvious deworming effect.

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Abstract

The invention discloses streptomyces griseus HL-50, and a complex microbial inoculant and application thereof. The strain class name of the streptomyces griseus HL-50 is Streptomyces griseus, the streptomyces griseus HL-50 is preserved in the general microbiology center of China microbial culture preservation management committee, and the preservation number is CGMCC No.18424. The bacterial colonyof the strain is in a round shape and in the color of light earthy yellow, the area is small, protrusions are in the middle, the surface of the bacterial colony is in a micro velvet shape, and is non-transparent, growth of aerial mycelium is rich and is milk white, the optimum growth temperature is 24 DEG C -35 DEG C, and the pH is 6-8. The invention further provides the complex microbial inoculant prepared from the streptomyces griseus HL-50 and eucalyptus multibract essential oil. The complex microbial inoculant can effectively repel various parasite infecting freshwater fish, the components are natural, the quality is good, microflora balance and health keeping in the bodies of the freshwater fish can further be promoted, the breeding cost is further lowered, and thus the complex microbial inoculant has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Streptomyces griseus HL-50 and its composite bacterial agent and application. Background technique [0002] In the breeding of aquatic animals, various parasites and bacteria will be caused due to incomplete cleaning of ponds, poor water quality management, and inadequate prevention, which will directly or indirectly cause huge losses to the entire aquaculture industry. In order to maintain the benefits of farming, antibacterial and insecticides have become just-needed products for aquaculture. However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosages, which will destroy the stability of water quality, damage the health of aquatic animals and reduce their immunity. In severe cases, it will directly lead to death. , which ultimately reduces production efficiency and brings safet...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K36/61A61P33/00A61P31/04C12R1/545
CPCA61K35/74A61K36/61A61P31/04A61P33/00C12N1/20C12N1/205C12R2001/545A61K2300/00
Inventor 高志强籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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