Preparation method and application of a milk-derived active peptide

An active peptide and milk-derived technology, applied in the direction of microbial-based methods, biochemical equipment and methods, applications, etc., can solve problems such as intestinal mucosal barrier dysfunction, low yield, intestinal structure and function damage, etc.

Active Publication Date: 2021-09-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under the conditions of nutrient deficiency, infection, stress and pathology, etc., animals can cause damage to intestinal structure and function, translocation of bacteria and endotoxin, and intestinal mucosal barrier dysfunction, etc., which seriously affect animal health and production performance. produce significant economic losses
[0003] At present, soybean protein or casein only adopts a single method of fermentation or enzymatic hydrolysis to prepare active peptides. Among them, the small peptides produced by enzymatic hydrolysis have a heavy bitter taste and poor palatability; the small peptides produced by microbial fermentation have a low yield and average Large molecular weight, it is difficult to fully exert potential biological functions

Method used

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  • Preparation method and application of a milk-derived active peptide
  • Preparation method and application of a milk-derived active peptide
  • Preparation method and application of a milk-derived active peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use soybean protein as raw material, add distilled water according to the ratio of soybean protein: water = 1:4, and pretreat at 85°C for 3 hours. Cool to 45°C, adjust the pH of the system to about 3.0 with 2mol / L HCl solution, add acid protease at an enzyme concentration of 5000 U per gram of raw material, keep the temperature at about 45°C, and react for 10 hours. Use casein as raw material, add distilled water according to the ratio of casein: water = 1:10, adjust the pH of the system to about 8.0 with 2mol / L NaOH solution, add trypsin according to the enzyme concentration of 1500U per gram of raw material, and maintain the temperature at 45 ℃, react for 2 hours. Mix 1 volume of soybean protein hydrolyzate with 2 volumes of casein hydrolyzate for later use. Prepare the fermentation medium according to the ratio of 4g of bran, 1g of soybean meal powder, 4g of ammonium sulfate, 0.3g of dipotassium hydrogen phosphate, 0.5g of calcium chloride, and 125ml of distilled wa...

Embodiment 2

[0022] Using soybean protein as raw material, add distilled water according to the ratio of soybean protein: water = 1:2, and pretreat at 90°C for 4 hours. Cool to 45°C, adjust the pH of the system to about 4.0 with 2mol / L HCl solution, add acid protease at an enzyme concentration of 5500 U per gram of raw material, keep the temperature at about 45°C, and react for 12 hours. Use casein as raw material, add distilled water according to the ratio of casein: water = 1:5, adjust the pH of the system to about 9.0 with 2mol / L NaOH solution, add trypsin according to the enzyme concentration of 2000U per gram of raw material, and maintain the temperature at 50 ℃, react for 5 hours. Mix 1 volume of soybean protein enzymatic hydrolyzate with 5 volumes of casein enzymatic hydrolyzate for later use. Prepare the fermentation medium according to the ratio of 4g of bran, 1g of soybean meal powder, 4g of ammonium sulfate, 0.3g of dipotassium hydrogen phosphate, 0.5g of calcium chloride, and ...

Embodiment 3

[0024] At the beginning of weaning of 25-day-old piglets, the control group was fed the control diet (see Table 1 for formula). The full-price feed of the test group was supplemented with 400 grams of milk-derived active peptide per ton of the diet of the control group, and the above-mentioned feed was fed until the age of 46 days, and the growth performance of piglets was measured.

[0025] Table 1 Test diet composition and nutritional indicators

[0026]

[0027]

[0028]The test results showed that compared with the control group, the average daily gain of the piglets in the experimental group increased by 31.67% (P0.05). Duodenal chymotrypsin, lipase and trypsin increased by 49.35% (P<0.05), 40.00% (P<0.05) and 38.40% (P<0.05) respectively compared with the control group. The spleen index increased by 32.00% (P<0.05), and the mandibular lymphatic index increased by 37.50% (P<0.05). Compared with the control group, serum IgG increased by 89.83% (P<0.01), and jejunum...

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Abstract

The invention discloses a preparation method and application of a milk-derived active peptide. Using soybean protein and casein as raw materials, a milk-derived active peptide product is obtained by combining enzymatic hydrolysis and microbial fermentation. Distilled water is added to soybean protein according to a certain mass ratio, soaked for pretreatment, pH of the system is adjusted, and acidic protease is added for reaction. Add distilled water to casein in a certain proportion, adjust the pH of the system, and add trypsin to react. Then soybean protein hydrolyzate and casein hydrolyzate are mixed, inoculated and fermented according to the ratio of 5%, the fermentation pH is controlled at about 7.0, the fermentation temperature is 40-50°C, and the fermentation time is 100-120 hours. After the fermentation is completed, it is dried at low temperature. Milk-derived active peptides for animals. Milk-derived active peptides have an important role in promoting the maturation and development of animal digestive tract functions. The milk-derived active peptides for animals of the present invention can significantly improve the feed intake and growth performance of livestock and poultry, and enhance the body's immunity and antioxidant capacity.

Description

technical field [0001] The invention belongs to the field of animal feed, and in particular relates to a preparation method and application of a milk-derived active peptide. Background technique [0002] The animal intestine is not only a place for digestion and absorption of nutrients, but also an important immune organ. Maintaining normal intestinal function is crucial to animal growth and development. Under the conditions of nutrient deficiency, infection, stress and pathology, etc., animals can cause damage to intestinal structure and function, translocation of bacteria and endotoxin, and intestinal mucosal barrier dysfunction, etc., which seriously affect animal health and production performance. Production brings significant economic losses. [0003] At present, soybean protein or casein only adopts a single method of fermentation or enzymatic hydrolysis to prepare active peptides. Among them, the small peptides produced by enzymatic hydrolysis have a heavy bitter tas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P21/06C12P21/00A23K50/30A23K50/75A23K50/60A23K20/147C12R1/225C12R1/685
CPCA23K20/147A23K50/30A23K50/60A23K50/75C12P21/00C12P21/06C12P39/00
Inventor 卢建军姚静宜董信阳冯杰郑有秀
Owner ZHEJIANG UNIV
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