Method for producing algae-derived saccharide with coupling and for purifying secondary tail water or sludge supernate

A technology of algae-derived sugar and tail water, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of compressed economic and ecological benefits, high cultivation costs, etc., to shorten the production cycle, reduce The effect of training costs

Pending Publication Date: 2019-12-20
EAST CHINA NORMAL UNIV
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Problems solved by technology

However, this method is only used in the field of algae resource utilization, and the large-scale cultivation of algae requires sufficient water and nutrients, which will make the cultivation cost too high and reduce its economic and ecological benefits.

Method used

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  • Method for producing algae-derived saccharide with coupling and for purifying secondary tail water or sludge supernate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The concentration of inorganic nitrogen (nitrate nitrogen, ammonia nitrogen) in the sludge supernatant of a sewage treatment plant is about 19.8 mg / L, and the concentration of phosphate is about 3.45 mg / L. The volume of reactor A is 10 L, the concentration of microalgae chlorophyll a is set to 4-5 mg / L, the light is 15000-20000 lx, the light-dark cycle is 16h:8h, the temperature is 28°C, the pH is adjusted to about 8, and aeration is performed. In order to ensure that the concentration of nitrogen and phosphorus in the effluent of reactor A basically meets the requirements of the quality standard for Class V functional waters in the Environmental Quality Standard for Surface Water (GB3838-2002), the hydraulic retention time is set to 4 days. Reactor A has been running continuously for about 30 days. After the initial 2-3 days of adaptation period, the effluent water quality is stable, including 0.4-1.3 mg / L of inorganic nitrogen and 93.4-98.0% removal rate; 0.10-0.25 mg / ...

Embodiment 2

[0028] The concentration of inorganic nitrogen (nitrate nitrogen, ammonia nitrogen) in the secondary tail water of a sewage treatment plant is about 5.1 mg / L, and the concentration of phosphate is about 0.52 mg / L. The volume of reactor A is 10 L, the concentration of microalgae chlorophyll a is set to 2.5-3.0 mg / L, the light is 10000-12000lx, the light-dark cycle is 14h:10h, the temperature is 28°C, the pH is adjusted to about 8, and aeration is performed. In order to ensure that the concentration of nitrogen and phosphorus in the effluent of reactor A basically meets the requirements of the quality standard for V functional waters in the Environmental Quality Standard for Surface Water (GB3838-2002), the hydraulic retention time is set to 1 day. Reactor A has been running continuously for about 30 days. After the initial 2-3 days of adaptation period, the effluent water quality is stable, including inorganic nitrogen 0.20-0.72 mg / L, removal rate of 85.9-96.0%; phosphate 0.03-0...

Embodiment 3

[0031] 1.2 g of the algae flour produced by the algae flour reactor B collected in Example 1 was directly dissolved in 40 ml of 5% (v / v) sulfuric acid solution to prepare a 30 g / L algae liquid. High temperature hydrolysis at 121°C for 30 min, the hydrolyzate was filtered through a 0.45 μm filter membrane, the hydrolyzate was adjusted to pH=6.5±0.2 with NaOH solid, Saccharomyces cerevisiae was added, and fermentation was carried out at 35°C at a speed of 150 r / min for 72 h. At the same time, the algae powder produced by reactor A was hydrolyzed and fermented under the same conditions.

[0032] 30 g / L Reactor B algae powder produced bioethanol concentration of 8.44 g / L, ethanol yield was 28.1 %, while 30 g / L Reactor A algae powder produced bioethanol concentration was only 0.93 g / L, ethanol yield was 3.1%. After being cultured in reactor B, the ethanol production rate of microalgae was significantly improved. The potential of the microalgae cultivated by this process to produc...

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Abstract

The invention discloses a method for producing algae-derived saccharide with coupling and for purifying secondary tail water or sludge supernate. The method comprises the following steps: 1) enablingsecondary tail water or sludge supernate to flow into a reactor A, and removing nitrogen and phosphorus in water by utilizing microalgae; 2) filtering the effluent of the reactor A, discharging the filtered effluent, discharging the concentrated high-concentration algae liquid into a reactor B, and enabling a proper amount of high-concentration algae liquid to flow back to the reactor A when the biomass of algae in the reactor A is insufficient; 3) supplementing nutrients and trace elements into the reactor B, and adding a photosynthesis promoter and a metabolic disruptor to promote the increase of saccharide; and 4) after the culture is finished, performing filtering or centrifuging to form a high-concentration algae solution, and performing drying to obtain the algae powder. The method combines the water quality improvement of the secondary tail water or the sludge supernate with the production of algae-derived saccharide, greatly reduces nitrogen and phosphorus in the effluent, alsosolves the problem of supply of a large amount of biomass in the production of the algae-derived saccharide, and effectively reduces the culture cost.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and bio-energy, and in particular relates to a method for coupling production of algae-derived sugar by using microalgae to deeply purify tail water or sludge supernatant of a sewage treatment plant. Background technique [0002] With the development of urbanization, the amount of urban domestic sewage has greatly increased, and the amount of tail water (including secondary tail water and sludge supernatant) discharged by urban sewage treatment plants is also very large. According to the "Pollutant Discharge Standards for Urban Sewage Treatment Plants (GB18918-2002)", even if the secondary tail water meets the discharge standards, the total nitrogen and total phosphorus content does not meet the "Environmental Quality Standards for Surface Water (GB3838-2002) "In accordance with the requirements of the quality standards for Class IV and V functional waters, the concentration of ni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C02F3/32C02F3/34C12R1/89C12R1/01C02F101/10C02F101/16
CPCC12N1/12C12N1/20C02F3/322C02F3/34C02F2101/16C02F2101/163C02F2101/105C02F2301/08C02F2305/06
Inventor 陈雪初黄莹莹洪婧洁刘思路雷少庭刘泽萱崔馨月徐力波戴禹杭
Owner EAST CHINA NORMAL UNIV
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