Algin oligosaccharide diacid composition

A technology of algin oligosaccharide and composition, which is applied in the field of algin oligosaccharide diacid composition, and can solve the problem of high production cost

Pending Publication Date: 2020-01-07
SHANGHAI GREEN VALLEY PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In order to obtain the anti-Alzheimer's disease and anti-diabetic mannooligosaccharide diacids disclosed in previous patents, it is necessary to remove the guluronic acid in the raw material alginic acid, and the content of guluronic acid in alginic acid is usually More than 30%, up to about 70%, so in order to obtain high-purity mannan oligosaccharide diacid, the actual production cost is very high

Method used

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  • Algin oligosaccharide diacid composition
  • Algin oligosaccharide diacid composition
  • Algin oligosaccharide diacid composition

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preparation example Construction

[0062] Preparation method of algin oligosaccharide diacid composition

[0063] The preparation process of algin oligosaccharide diacid of the present invention is summarized as follows:

[0064] After preliminary degradation of alginic acid, a mixed polysaccharide of polymannuronic acid and polyguluronic acid can be obtained, and the mixed polysaccharide can be precipitated by acid method to remove a certain amount of polyguluronic acid; The higher the pH value control, the higher the polyguluronic acid content in the obtained mixed polysaccharide. For example, refer to the methods disclosed in Chinese Patent Application No.98806637.8 and CN02823707.2. In the presence of an oxidizing agent, the mixed polysaccharides described above undergo oxidative degradation of sugar chains to obtain oxidized oligosaccharides with different degrees of polymerization. The oxidized oligosaccharides are characterized in that mannuronic acid or guluronic acid at the reducing end of the oligo...

Embodiment 1

[0164] Step 1): Preparation of algin oligosaccharide mixture

[0165] Make a 10% solution of 5Kg sodium alginate, add dilute hydrochloric acid to adjust the pH to about 3.0, raise the temperature to 80°C, stir, react for 10 hours, stop heating, cool to room temperature, add NaOH to adjust the pH to 9.0, then add Adjust the pH to 3.2 with dilute hydrochloric acid, centrifuge at 5000 rpm for 10 min, collect the supernatant, concentrate with a rotary evaporator, and dry in vacuum to obtain 1500 g of the intermediate.

[0166] The NMR spectrum of the intermediate is attached figure 1 . The NMR determination method is as follows: Sample preparation: Weigh 30 mg of the sample to be tested and dissolve it in 0.5 ml D2O, freeze-dry, add 0.5 ml deuterated heavy water to dissolve, freeze-dry again, and finally dissolve the freeze-dried sample powder with an appropriate amount of heavy water Transfer to an NMR tube, prepare a 100 mg / ml solution to be tested, and add 0.01% (w / v) deutera...

Embodiment 2

[0191] Weigh 100g of commercially available sodium alginate (purchased by Sinopharm Reagent Network, CAS No. 9005-38-3, specification CP, Shanghai test), add distilled water and mix evenly, after swelling, make a solution with a volume of 1L, and adjust the pH to 4.0 with NaOH , Reaction at room temperature 25°C. Adjust the gas flow at the outlet of the oxygen cylinder and the power of the ozone generator so that the flow rate of the ozone mass concentration reaches 1g / hr, and it is passed into the reaction solution. Stop feeding ozone after reacting for 10 hours, add appropriate amount of water to adjust the concentration of the solution to about 15%, filter with an ultrafiltration membrane with a molecular weight cut-off of 1000 Da, collect the permeate, concentrate with a rotary evaporator, and dry in vacuo to obtain 80 g of product B.

[0192] Superdex peptide (GE Company) size exclusion chromatography combined with multi-angle laser light scattering (MALS, Wyatt Company) ...

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Abstract

The invention relates to an algin oligosaccharide diacid combination. The algin oligosaccharide diacid combination contains D-mannuronic acid or pharmaceutically-accpetable salt thereof in formula (III), wherein n is selected from an integral number from 1-9, m is selected from 0,1 or 2, m' is selected from 0 or 1, besides, the total weight of the algin oligosaccharide diacid of which n=1-5 is 60%or above of the total weight of the combination, and the total weight of L-guluronic acid is 50% or below of the weight of the composition.

Description

technical field [0001] The invention relates to obtaining the optimal composition of algin oligosaccharide diacid through a biological activity screening method. The method adopts an animal model of senile dementia to evaluate the influence of different polymerization degrees and ratios of algin oligosaccharide on biological activity. Finally, the composition with the best biological activity is screened and the desired target substance is prepared by ultrafiltration membrane separation. Background technique [0002] Alginate oligosaccharides have received extensive attention due to their potential medicinal value. Alginate oligosaccharides are usually prepared from alginic acid through multiple steps. [0003] In the alginate oligosaccharide molecule, there is an M segment formed by linking mannuronic acid (D-mannuronic acid) through β-1,4-glycosidic bonds, and guluronic acid (L-guluronic acid) through α-1 , the G segment formed by 4-glycosidic linkage, and the MG segment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61K31/715A61P25/28A61P29/00A61P25/16A61P3/10A23L33/125
CPCA61K31/7032A61K31/715A61P25/28A61P29/00A61P25/16A61P3/10A23L33/125A23V2002/00A61K2300/00A23V2200/328A23V2200/30A23V2200/322A61K31/702A61P1/00A61P19/02A61K31/7012A61P25/04
Inventor 耿美玉张真庆晋迎申肖中平丁健
Owner SHANGHAI GREEN VALLEY PHARMA
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