Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A nucleic acid remover

A nucleic acid and oxalic acid technology, applied in the field of molecular biology, can solve problems such as corrosiveness and irritation, and achieve the effects of simple and convenient operation, mild odor and long storage period

Active Publication Date: 2021-08-10
青岛立见生物科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nucleic acid removal agent developed by the present invention can effectively solve the problems of corrosion, irritation, high efficiency and stability encountered in the process of nucleic acid degradation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A nucleic acid remover
  • A nucleic acid remover

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A nucleic acid remover, containing the following components and mass percentage: 5% hydrogen peroxide, 0.1% sodium hypochlorite, 0.1 oxalic acid, 0.1% acetic acid, 1% AEO-9, the rest is made up with deionized water, and the final pH value is adjusted to 3.5 .

Embodiment 2

[0023] A nucleic acid remover, containing the following components and mass percentage: 10% hydrogen peroxide, 3% sodium hypochlorite, 3% oxalic acid, 2% acetic acid, 8% AEO-9, the rest is made up with deionized water, and the final pH value is adjusted 3.5.

Embodiment 3

[0025] Utilize the nucleic acid removal agent described in embodiment 1 and embodiment 2 to degrade the genome, the specific method is as follows:

[0026] (1) DNA / RNA genome extraction kit to extract nucleic acid

[0027] ①Take 0.2 g of pig liver sample, add 5 times the weight of normal saline, grind it evenly, add 20 μL of proteinase K solution, mix well, and lyse in a water bath at 56°C for 15 minutes;

[0028] ② Centrifuge at 12,000 rpm at room temperature for 5 minutes, take 200 μL of the supernatant and add it to a new centrifuge tube, then add 200 μL of buffer solution, mix thoroughly by inversion, and place at 70°C for 10 minutes;

[0029] ③ Add 200 μL of absolute ethanol, shake and mix well for 15 seconds;

[0030] ④ Add the above solution into an adsorption column, centrifuge at 12,000 rpm for 30 seconds, discard the waste liquid, and put the adsorption column back into the collection tube;

[0031] ⑤ Add 500 μL of washing solution to the adsorption column, centrif...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a nucleic acid removing agent, which belongs to the field of molecular biology and contains the following components and volume percentages: 5%-10% of an oxidant, 0.1%-3% of a chlorine-containing reagent, 0.1-3% of an acid-containing reagent, and 0.1% of a stabilizer ‑2%, surfactant 1%‑8%, and the balance deionized water. The nucleic acid remover is odorless, mild in nature and has no stimulating effect. It is simple and convenient to operate, convenient to store, and has a long storage period. When in use, the nucleic acid remover can be directly sprayed on the laboratory floor, wall, desktop, countertop of a biological safety cabinet, mobile The surface of the liquid container, the outer surface of various instruments, the outer wall of the centrifuge tube, etc., can also be directly wiped with the nucleic acid remover on the instrument, countertop, desktop, etc. This reagent can effectively degrade nucleic acid fragments within a few minutes and prevent laboratory aerosol pollution.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a nucleic acid removing agent. Background technique [0002] Since its inception in 1985, PCR technology has been widely used in all life-related sciences such as medicine, forensic science, agronomy, and biology, and has become one of the necessary basic technologies in the field of life research. In particular, the fluorescent PCR technology developed based on ordinary PCR technology is more than 100 times more sensitive than ordinary PCR, and is favored by a large number of scientific researchers. It has become a common method for the diagnosis of disease pathogens in the fields of medicine and veterinary medicine. But at the same time, the unique principle of PCR technology tells us that billions of molecules will be produced after one molecule undergoes PCR reaction. This high sensitivity means that the environment and laboratory are extremely sensitive during th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C11D1/72C11D1/66C11D1/29C11D3/20C11D3/39C11D3/04C11D3/10C11D3/60C11D11/00C07H21/04C07H1/00B01D53/78B01D53/72
CPCC11D1/72C11D1/008C11D1/662C11D1/29C11D3/2079C11D3/2082C11D3/3942C11D3/046C11D3/10C07H21/04C07H1/00B01D53/78B01D53/72B01D2251/106B01D2251/108B01D2251/70B01D2257/704C11D2111/24C11D2111/14
Inventor 张志官丽娟宫枫举李翠翠孙学强
Owner 青岛立见生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com