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Application of camellia oleifera flesh fruit polysaccharides to preparation of drugs or health-care products for preventing and treating II-type diabetes

A technology of diabetes drug and fructose polysaccharide is applied in the field of traditional Chinese medicine to achieve the effects of rich medicinal material resources, good taste and no toxic and side effects

Active Publication Date: 2020-01-17
GANNAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the treatment methods for diabetes include insulin injection and oral hypoglycemic drugs, but these methods also cause some side effects during clinical application, such as high doses of insulin and oral hypoglycemic drugs can cause hypoglycemia, liver damage, lactic acid poisoning and diarrhea

Method used

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  • Application of camellia oleifera flesh fruit polysaccharides to preparation of drugs or health-care products for preventing and treating II-type diabetes
  • Application of camellia oleifera flesh fruit polysaccharides to preparation of drugs or health-care products for preventing and treating II-type diabetes
  • Application of camellia oleifera flesh fruit polysaccharides to preparation of drugs or health-care products for preventing and treating II-type diabetes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of camellia oleifera pulp fructopolysaccharide

[0030] (1) Dried and pulverized the fleshy fruit of Camellia oleifera for later use. Weigh 30g of dried Camellia oleifera fleshy fruit into a 2L round bottom flask, add 900mL of pure water to soak overnight at room temperature, extract with water at 99°C±1°C for 2 hours, then filter with four layers of gauze, and the filtered medicinal residue is 99± Repeat the water extraction at 1°C for 2 hours, and combine the extracts.

[0031] (2) In the extract prepared in step 1, add 500 mL of absolute ethanol to precipitate, centrifuge to collect the precipitate, use the Sevage method to remove protein, decolorize, and dialyze to obtain the crude product of camellia oleifera fructopolysaccharide (CFFP), and use anthrone- Sulfuric acid method and ultraviolet spectrophotometer were used to measure the fructopolysaccharide content of Camellia oleifera flesh to be 40.30%.

Embodiment 2

[0032] Embodiment 2: Mouse animal experiment of type Ⅱ diabetes

[0033] 1. Experimental protocol

[0034] 1.1 Drug preparation

[0035] Take the camellia oleifera pulp fructose polysaccharide prepared above, and mix it with water to prepare camellia oleifera pulp fructose polysaccharide solutions with concentrations of 12.5 mg / mL and 25 mg / mL respectively. In addition, metformin tablets are ground into powder and prepared with a concentration of 20 mg / mL. mL of metformin solution.

[0036] 1.2 Establishment of animal model of type Ⅱ diabetes

[0037] Fifty 10-week-old C57 mice were purchased from Hunan Slack Experimental Animal Co., Ltd. and raised in the Experimental Animal Center of Gannan Medical College. 10 rats, one of which was the normal control group, fed with normal feed, and the other four groups were induced to establish type Ⅱ diabetes mouse models with high-sugar and high-fat diet plus small doses of streptozotocin (STZ), and 72 hours after administration and...

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Abstract

The invention discloses application of camellia oleifera flesh fruit polysaccharides to preparation of drugs or health-care products for preventing and treating II-type diabetes. The camellia oleiferaflesh fruit polysaccharides are pure natural plant polysaccharides extracted and purified from camellia oleifera flesh fruits. By carrying out a diabetic mouse animal experiment, blood glucose of mice is lowered significantly after drug administration through the camellia oleifera flesh fruit polysaccharides for three months, the area under a curve of glucose tolerance and the area under a curveof insulin tolerance are lower significantly, a series of renal pathological detection and analysis of HE, PAS staining, immunohistochemistry, transmission electron microscope results, renal antioxidation and anti-inflammation, renal hypertrophy and metabolism situations show that a series of pathological changes of kidneys of diabetic mice are all improved. Therefore, through the experiment, it is proved that the camellia oleifera flesh fruit polysaccharides have good effects of prevention and treatment of II-type diabetes and renal protection.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to extracting and purifying oleifera oleifera fructopolysaccharide from oleifera oleifera succulent fruit as a raw material and its application in preparing type 2 diabetes prevention and treatment medicine or health care product. Background technique [0002] Diabetes has become the third leading cause of death after cardiovascular disease and cancer. Among people over 20 years old in my country, the overall prevalence of diabetes is 11.6%, and an estimated 113.9 million adults suffer from diabetes. The incidence of diabetes is gradually increasing all over the world, and the incidence of type Ⅱ diabetes is the most obvious. Dysfunction and reduction of islet β cells are the main pathogenesis of type Ⅱ diabetes. Regeneration and restoration of the number of islet β cells to restore the function of islet β cells is fundamental to the treatment of type Ⅱ diabetes. [0003...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61K36/82A61P3/10A61P13/12
CPCA61K31/715A61K36/82A61P3/10A61P13/12A61K2236/331A61K2236/39A61K2236/53Y02A50/30
Inventor 吴素珍李加林刘志平
Owner GANNAN MEDICAL UNIV