Preparation method of agarose boric acid affinity material suitable for fish tropomyosin purification

A tropomyosin and agarose technology, applied in the field of separation, can solve the problems of unable to obtain tropomyosin, unable to obtain ideal effects and the like, and achieve the effects of shortening purification time, simplifying purification steps and broad application prospects
CN110711571AActive Publication Date: 2020-01-21OCEAN UNIV OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
OCEAN UNIV OF CHINA
Publication Date
2020-01-21

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Abstract

The invention relates to the technical field of separation. The invention discloses a preparation method of an agarose boric acid affinity material suitable for fish tropomyosin purification. The novel agarose boric acid affinity material is prepared by taking 3,5-difluoro-4-formylphenylboronic acid as a functional monomer, tri (2-aminoethyl)amine as a multi-branched ligand and agarose microspheres as a matrix material, and is used for separation and purification of fish tropomyosin for the first time, wherein optimal use conditions are determined by using different fish samples. Results showthat when the agarose concentration is 3%, the pH value of the loading equilibrium liquid is 7.4 and the concentration of the HAc eluent is 100 mM, the purity of the obtained tropomyosin reaches 90% or above, and the column capacity reaches 1.85 mg / mL or above. Compared with a traditional method, the method can remarkably shorten the purification time (shortened from several days to 3-4 hours), noorganic solvent is used, and the product purity is equivalent to that of the traditional method.
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Description

technical field

[0001] The invention relates to the field of separation technology, in particular to a method for preparing an agarose boronic acid affinity material suitable for the purification of fish tropomyosin. Background technique

[0002] Food allergy is one of the main problems of public health security at present. According to the statistics of WHO / IUIS allergen database, tropomyosin is the main allergen in many invertebrates (such as: shrimp, crab, shellfish). However, in recent years, tropomyosin in some fish (such as: tilapia, monkfish, bluefin tuna, albacore tuna, zebrafish, etc.) potential allergens, which has aroused widespread concern. Therefore, an in-depth understanding of the sensitization mechanism of tropomyosin in fish and other aquatic products, and reasonable monitoring and labeling of tropomyosin in different foods are extremely important for effectively controlling the allergic safety problems caused by tropomyosin. important.

[0003] Efficient...

Claims

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