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Polymer composite hydrogel for treating bladder tumor and preparation method and application thereof

A composite hydrogel and bladder tumor technology, which is applied in the direction of antineoplastic drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem that active substances are easily diluted by urine Discharge and other issues, to achieve the effect of benefiting the freeze-drying efficiency, promoting drug penetration, and slowing down the dissolution rate

Inactive Publication Date: 2020-02-11
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is the problem that active substances in the bladder are easily diluted by urine and easily discharged with urine. A polymer composite hydrogel for treating bladder tumors, a preparation method and an application are provided to improve bladder wall Wait for the cell interaction and drug absorption of the mucosal tissue, prolong the drug action time, achieve a sustained release effect, and make the infusion drug delivery method more effective in treating bladder tumors

Method used

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  • Polymer composite hydrogel for treating bladder tumor and preparation method and application thereof
  • Polymer composite hydrogel for treating bladder tumor and preparation method and application thereof
  • Polymer composite hydrogel for treating bladder tumor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the preparation of metformin chitosan hydrogel

[0049] Dissolve 0.2g of chitosan powder with a molecular weight of 50kD and 85% degree of deacetylation in 10ml of 0.1mol / l hydrochloric acid solution, mix well and fully stir to obtain an acidic solution of chitosan, the acidic solution is at speed 9000rpm, time 3min, temp Centrifuge at 10°C to remove solid impurities to obtain a clear solution, put the solution into a -80°C refrigerator for pre-freezing for 8 hours, and then freeze-dry for 8 hours to obtain a chitosan freeze-dried powder; mix 25 mg of chitosan freeze-dried powder with 1 ml of physiological saline with a metformin concentration of 80 mM was mixed to obtain the polymer composite hydrogel.

[0050] Drug-loaded chitosan hydrogel UV spectrum has obvious metformin absorption peak at 233nm (such as figure 1 ), the characteristic peaks of chitosan and metformin also appeared on the infrared spectrum (such as figure 2 ), its pH is weakly acidic ...

Embodiment 2

[0051] Embodiment 2: the preparation of metformin chitosan hydrogel

[0052] Compared with Example 1, the only difference is that the polymer material in this example is chitosan powder with a molecular weight of 200KD and the feeding amount of chitosan freeze-dried powder is 10 mg.

[0053] Drug-loaded chitosan hydrogel UV spectrum has obvious metformin absorption peak at 233nm (such as figure 1 ), its pH is weakly acidic (such as image 3 ), slightly irritating to the bladder mucosa, and can tolerate multiple administrations. Zeta potential value is positive (such as Figure 4a ), has a certain stability, and can attract each other with the negatively charged bladder mucosa through electrostatic force. (Such as Figure 5 ).

experiment example 1

[0055] Drug-loaded chitosan gel animal experiments:

[0056] Establishment of orthotopic mouse bladder cancer model: Healthy female C57BL-6 mice, 6 weeks old, were observed in the quarantine room for 1 week and then randomly divided into groups, with 3 mice in each group, and MB49 cells that grew well in the logarithmic growth phase were selected. , after digestion, centrifugation, making a single cell suspension, counting and determining the cell density, and implanting bladder cancer cells into mice.

[0057] Bladder perfusion: the mice were randomly divided into 5 groups, I-implanted tumor control group (Ctrl), II-bladder perfusion metformin, the dose was 80mM, 50 μl once (Met), III-perfusion 200KD drug-containing chitosan preparation, The dose is 80 μM, 50ul once (Cs); IV—blank chitosan preparation, 50 μl once (Cs+Met), V—no tumor-implanted blank control group (Normal). Drug treatment was started on the second day after tumor implantation, once a week for 2 weeks, and the...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to polymer composite hydrogel for treating bladder tumor and a preparation method and application. The composite hydrogel comprises freeze-dried polymer material powder, guanidine active substances for treating bladder tumor and normal saline, wherein the polymer material is one or a mixture of chitosan and chitin.The composite hydrogel improves the cell interaction and drug absorption of mucous membrane tissues such as bladder walls, prolongs the drug action time, achieves the slow release effect, and enablesthe effect of treating bladder tumors in a perfusion administration mode to be better.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a polymer composite hydrogel for treating bladder tumors, a preparation method and application. Background technique [0002] Bladder cancer refers to malignant tumors that occur on the bladder mucosa. It is the most common malignant tumor of the urinary system and one of the ten most common tumors in the whole body. With the aggravation of environmental pollution, the incidence of bladder cancer is increasing year by year, and the incidence of genitourinary system tumors in my country is at a high level. Although transurethral resection has become the standard treatment, recurrence and metastasis are common clinically. [0003] In order to prevent recurrence, intravesical chemotherapy or adjuvant immunosuppressant therapy is usually carried out. The commonly used intravesical chemotherapy drugs are mitomycin, bacillus Calmette-Guerin, pirarubicin, etc. These drugs h...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/36A61K31/155A61P35/00
CPCA61K9/06A61K47/36A61K31/155A61P35/00
Inventor 杨小平胡争胡鑫邓俊周思纯补译徵郑萌盟吴景涛刘晓君陈思馨
Owner HUNAN NORMAL UNIVERSITY
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