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Bacillus amyloliquefaciens and method for preparing gamma-polyglutamic acid fertilizer synergist by adopting bacillus amyloliquefaciens

A technology for dissolving amyloliquefaciens and fertilizer synergists, which is applied in the fields of Bacillus amyloliquefaciens, preparation of fertilizer synergists, and preparation of γ-polyglutamic acid fertilizer synergists, can solve the problem of environmental improvement and production increase and value-added unhelpful, γ- - Low yield of polyglutamic acid, increased agricultural production costs, etc., to achieve the effect of reducing the occurrence of various soil-borne diseases, improving product quality, and reducing loss

Inactive Publication Date: 2020-02-14
杨凌未来中科环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still defects in the large-scale use of this type of water retaining agent, such as polyacrylamide soil water retaining agent, which will significantly increase the cost of agricultural production, and whether it will cause irreversible impact on the environment during the year-round use remains to be verified
Although the composition disclosed in this patent contains ingredients such as polyglutamic acid or polyaspartic acid, it still only acts as a soil water-retaining agent, and cannot exert a slow-release and synergistic effect on fertilizers. Purpose, that is, to reduce the use of chemical fertilizers, it does not help to improve the environment and increase production and value
[0008] In summary, most of the production of γ-polyglutamic acid organic fertilizers is based on the fermentation of a single strain of γ-polyglutamic acid, and the yield of γ-polyglutamic acid is low, so it is urgent to develop a γ-polyglutamic acid fertilizer. -New strain with high yield of polyglutamic acid and method for producing gamma-polyglutamic acid with high yield by using the strain
And further, for the development of γ-polyglutamic acid fertilizer synergists, technological breakthroughs are urgently needed

Method used

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  • Bacillus amyloliquefaciens and method for preparing gamma-polyglutamic acid fertilizer synergist by adopting bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens and method for preparing gamma-polyglutamic acid fertilizer synergist by adopting bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens and method for preparing gamma-polyglutamic acid fertilizer synergist by adopting bacillus amyloliquefaciens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing gamma-polyglutamic acid fertilizer synergist

[0032] 1. Breeding of γ-polyglutamic acid-producing strain JX8-2

[0033] Weigh 10g of the soil sample containing the target bacterial strain, add it into a triangular flask filled with 90ml sterile water, shake it well, and dilute the resulting suspension by 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 Concentration gradients were diluted sequentially, and then 0.1ml was taken from the dilutions of each concentration, spread on agar medium, and placed in a constant temperature incubator at 35°C for 24-28h. The well-growing colony was selected and cultured repeatedly by streaking for more than three times, and the strain was obtained after purification. The medium components are: peptone 10g, yeast extract powder 5g, glucose 10g, NaCl 5g, agar 20g, pH 7.0, water up to 1000ml.

[0034] 2. Sterilize the beef extract peptone solid medium and nitrogen fixation medium (CM0003) for later use. The components...

Embodiment 2

[0049] Field application test of γ-polyglutamic acid fertilizer synergist on pakchoi planting.

[0050] 1. Test time: from May 5, 2017 to June 10, 2017;

[0051] 2. Test site: Test base of Yangling Vocational and Technical College, Yangling Demonstration Area, Shaanxi Province;

[0052] 3. Experimental crop: Chinese cabbage;

[0053] 4. Test method

[0054] A total of 3 treatments were set up in the experiment, arranged in random blocks, repeated 3 times, and the area of ​​the plot was 60m 2 .

[0055] Test group 1: conventional fertilization;

[0056] Test group 2: 60% conventional fertilization + fine sand;

[0057] Test group 3: 60% conventional fertilization + γ-polyglutamic acid fertilizer synergist (2kg per mu);

[0058] The experiment was carried out on the basis of local conventional fertilization. Conventional fertilization is: 45% organic-inorganic compound fertilizer (15-15-15) 25Kg per mu before transplanting of seedlings. Before the seedlings were transpla...

Embodiment 3

[0071] Field application test of γ-polyglutamic acid fertilizer synergist on tomato planting

[0072] 1. Test time: from May 25, 2017 to September 5, 2017;

[0073] 2. Test location: Northwest Agriculture and Forestry University Test Base in Yangling Demonstration Zone, Shaanxi Province (the soil type of the field is fluvo-aquic soil, the texture is irrigated soil, the fertility is medium to high, and the fertility is uniform);

[0074] 3. Test crop: tomato;

[0075] 4. Test method

[0076] A total of 3 treatments were set up in the experiment, arranged in random blocks, repeated 3 times, and the area of ​​the plot was 30m 2 .

[0077] Process 1: conventional fertilization+mu use γ-polyglutamic acid fertilizer synergist 4kg to mix dry fine soil 20kg and sprinkle after seedling transplanting;

[0078] Process 2: conventional fertilization+mu with fine sand 4kg mix dry fine soil 20kg and spread after seedling transplanting;

[0079] Treatment 3: regular fertilization.

[0...

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Abstract

The invention discloses a strain of bacillus amyloliquefaciens JX8-2. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the address of the center is: Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, 100101, China, the preservation number is CGMCC No.14775, and the preservation date is October 9, 2017. The invention also discloses application of the strain in preparation of a gamma-polyglutamic acid fertilizer synergist, and a method for preparing the gamma-polyglutamic acid fertilizersynergist by using the strain and paenibacillus mucilaginosus.

Description

technical field [0001] The invention relates to a strain of Bacillus, in particular to a Bacillus amyloliquefaciens, and the invention also includes the use of the Bacillus in the preparation of fertilizer synergists, and the use of the bacteria to prepare γ-polyglutamic acid fertilizer synergists agent method. Background technique [0002] my country is a big country that uses chemical fertilizers, but the utilization rate of fertilizers is generally low. According to statistics, the seasonal utilization rate of nitrogen fertilizer is about 30-35%, the seasonal utilization rate of phosphorus fertilizer is about 10-25%, and the seasonal utilization rate of potassium fertilizer is about 40-50%. , There is still a gap between this utilization rate and the level of developed countries in the world. The loss of a large amount of fertilizer not only causes huge economic losses, but also causes serious environmental pollution. Specifically, the pollution of the environment caused...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/02C05F11/08C05G1/00C12R1/07
CPCC05C11/00C05F11/08C12P13/02C12N1/205C12R2001/07C05D9/02C05F11/02C05F11/10C05G1/00
Inventor 王森林吴彦
Owner 杨凌未来中科环保科技有限公司
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