Anti-infection tissue engineering skin scaffold and preparation method thereof

Abstract

The invention discloses an anti-infection tissue engineered skin bracket. The bracket has an upper and lower double-layer structure, and the layers are combined through glutaraldehyde cross-linking, and has good mechanical strength. The upper layer of epidermal scaffold polycaprolactone-polyethylene glycol-polycaprolactone / nanosilver / chitosan nano-gauze layer inside the fiber nano-silver and chitosan on the surface of the fiber synergistically exert antibacterial effect, the lower layer of dermal scaffold silk fibroin / Zinc acetate is evenly distributed in the hyaluronic acid fiber, which can inhibit bacterial aggregation and exert anti-infection effect; the lower layer is a nanofiber bundle braided scaffold, and the structure, pore size and porosity of the fiber bundle scaffold can be adjusted freely through weaving, and can be prepared The fiber bundles are arranged in an orderly and regular manner, and the average pore size is close to the size of the cells, so that the cells can enter and guide the directional growth of the tissue.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an anti-infection tissue engineering skin scaffold and a preparation method thereof. Background technique [0002] As the largest organ of the human body, the skin plays a role in protecting the human body from the invasion of external objects. Damage to the skin can lead to the invasion of bacteria and pathogens, and severe cases can lead to death. Therefore, in the past three decades, a large number of studies on skin scaffolds have been carried out in the fields of biomedical materials and tissue engineering. Human skin is a double-layered structure consisting of epidermis and dermis. The epidermis is mainly composed of dense keratinocytes, which play a protective role; the dermis is mainly composed of fibroblasts and the collagen secreted by them, which has good elasticity and mechanical strength, and plays an important role in the skin regeneration process. ...

AI Technical Summary

Problems solved by technology

However, the nanofibrous scaffold prepared by electrospinning has disordered fiber arrangement and cannot play a guiding role in cell adhesion and migration.

Most of the electrospun fiber membranes are easy to shrink and deform, and the fiber stability is insufficient. When used as an epidermal scaffold for skin tissue engineering, it cannot meet the requirements of structural stability; the dermal scaffold structure has a small nanofiber pore size and a dense structure, which is not conducive to cell regeneration. Access to the inside of the scaffold to form a three-dimensional culture

When preparing scaffolds by 3D printing, although the porosity and pore size can be adjusted, which solves the problem of compact structure of electrospun fibers, since the printing materials need to reach a certain hardness to obtain the designed shape and structure, this results in the application of 3D printing scaffolds. When the skin tissue engineering is too hard, it is not easy to adhere to the wound surface, which is not conducive to cell adhesion and migration

[0004] Skin and soft tissue damage can easily lead to infection, aggravate wound ulceration, and affect tissue repair. Therefore, at present, antibiotics such as gentamicin and cephalosporins are often loaded into tissue engineering skin scaffold materials to improve their anti-infection ability. However, if the dosage of antibiotics is too large and the release concentration is high, it will cause tissue damage

Images