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Agricultural microbial agent with nitrogen-fixing, phosphorus-dissolving, potassium-dissolving and cellulose-dissolving functions and preparation method thereof

A microbial agent and cellulose technology, applied in the field of microbial agents, can solve the problems of lack of diversity in function, single strain, low decomposition efficiency, etc., and achieve the effect of not being easily inhibited, low in nutritional conditions, and promoting plant growth

Inactive Publication Date: 2020-04-10
WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current domestic agricultural microbial agents use a single strain of bacteria, lack of diversity in function, and low decomposition efficiency, and most of them use the original powder of bacteria, which need to grow into hyphae after germination, and then express various enzymes to decompose For large organic compounds, this process may take several days

Method used

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  • Agricultural microbial agent with nitrogen-fixing, phosphorus-dissolving, potassium-dissolving and cellulose-dissolving functions and preparation method thereof
  • Agricultural microbial agent with nitrogen-fixing, phosphorus-dissolving, potassium-dissolving and cellulose-dissolving functions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides an agricultural microbial bacterial agent with the functions of nitrogen fixation, phosphorus, potassium, and cellulose, including the following components in terms of mass percentage: 30% of Aspergillus niger fermentation product, 20% of Azotobacter rotundum bacteria powder, Trichoderma reesei fermentation product 25%, Trichoderma harzianum fermentation product 20%, sucrose 2%, glutamic acid 1%, white carbon black 2%.

[0037] The preparation method of above-mentioned agricultural microbial inoculum, comprises the steps:

[0038] 1) Crushing: Preliminarily crush the straw, soybean meal and rice husk respectively, and after passing through a 40-mesh sieve, mix the straw powder, soybean meal powder and rice husk powder at a mass ratio of 14:5:3 to obtain solid raw materials;

[0039] 2) Dissolving: adding sucrose, ammonium sulfate, sodium chloride and potassium dihydrogen phosphate to water and dissolving at 25°C for 30 minutes to form a sucrose-...

Embodiment 2

[0046] This embodiment provides an agricultural microbial agent with the functions of nitrogen fixation, phosphorus, potassium, and cellulose, including the following components by mass percentage: 20% of Aspergillus niger fermentation product, 20% of silicate bacteria powder, Trichoderma reesei fermentation product 25%, Trichoderma harzianum fermentation product 20%, maltose 5%, polyaspartic acid 5%, white carbon black 5%.

[0047] The preparation method of above-mentioned agricultural microbial inoculum, comprises the steps:

[0048] 1) Crushing: Preliminarily crush the straw, soybean meal and rice husk respectively, and after passing through a 40-mesh sieve, mix the straw powder, soybean meal powder and rice husk powder at a mass ratio of 12:6:5 to obtain solid raw materials;

[0049] 2) Dissolving: adding sucrose, ammonium sulfate, sodium chloride and potassium dihydrogen phosphate into water and dissolving at 20°C for 30 minutes to form a sucrose-containing inorganic salt...

Embodiment 3

[0056] This embodiment provides an agricultural microbial agent with the functions of nitrogen fixation, phosphorus, potassium, and cellulose, including the following components by mass percentage: 25% of Aspergillus niger fermentation product, 20% of silicate bacteria powder, Trichoderma reesei fermentation product 25%, Trichoderma harzianum fermentation product 15%, maltose 2, glucose 3%, polyglutamic acid 5%, white carbon black 5%.

[0057] The preparation method of above-mentioned agricultural microbial inoculum, comprises the steps:

[0058] 1) Crushing: Preliminarily crush the straw, soybean meal and rice husk respectively, and after passing through a 40-mesh sieve, mix the straw powder, soybean meal powder and rice husk powder at a mass ratio of 16:4:2 to obtain solid raw materials;

[0059] 2) Dissolving: adding sucrose, ammonium sulfate, sodium chloride and potassium dihydrogen phosphate to water and dissolving at 30°C for 30 minutes to form a sucrose-containing inorg...

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Abstract

The intention belongs to the field of microbial agents, and relates to an agricultural microbial agent with nitrogen-fixing, phosphorus-dissolving, potassium-dissolving and cellulose-dissolving functions, the agricultural microbial agent comprises the following components in percentage by mass: 20-30% of aspergillusniger fermentation product, 10-20% of azotobacter powder, 15-25% of trichodermareesei fermentation product and 10-20% of trichodermaharzianum fermentation product. 1-5% of carbohydrate, 1-5% of white carbon black and 1-5% of at least one of amino acids and polypeptides; The method comprises the following steps: firstly, mixing solid raw materials with an inorganic salt solution containing cane sugar and then sterilizing to prepare a sterile culture medium; inoculating aspergillusniger spore powder, trichodermaharzianum spore powder and trichodermareesei spore powder into a sterile culture medium for fermentation; and finally, uniformly mixing the amino acids or polypeptideswith the aspergillusniger fermentation product, the trichodermaharzianum fermentation product, the trichodermareesei fermentation product, the azotobacter powder, the carbohydrate and the white carbonblack. The microbial agent provided by the invention can activate beneficial elements such as phosphorus, calcium and the like in soil while rapidly decomposing straw in the soil, and fix nitrogen inair, so that the purpose of promoting crop growth is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to an agricultural microbial bacterial agent with functions of nitrogen fixation, phosphorus, potassium, and cellulose decomposing and a preparation method thereof. Background technique [0002] Straw and plant residues are the by-products of the crop production process, which contain organic matter, nitrogen, phosphorus, potassium and trace elements, among which the organic matter content is the majority, but the organic matter in the straw is mainly cellulose, hemicellulose and lignin Most of them have the most cellulose content. Cellulose is mainly composed of glucose connected by β-1,4 glycosidic bonds, which need to be hydrolyzed into glucose by cellulase to be directly used by cells. The cellulose effectively used by humans in nature accounts for only a small part of the total cellulose, and most Most cellulose is not used by humans. [0003] A...

Claims

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Application Information

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IPC IPC(8): C05G3/80C05F17/20C12N1/20C12N1/14C12R1/065C12R1/685C12R1/885
CPCC05C11/00C05F11/08C12N1/14C12N1/20Y02W30/40
Inventor 高琪陈娇尹涛林春鸿陶佩文杨婷吕为
Owner WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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