Production process of full-enzyme polypeptide for fertilizer

Abstract

The invention relates to the technical field of polypeptide production, and in particular relates to a production process of full-enzyme polypeptide for a fertilizer. The production process comprisesthe steps of 1) current-assisted swelling: putting protein into a swelling solution, introducing constant-voltage forward current for treatment, and introducing constant-voltage reverse current for treatment; (2) ultrasonic-assisted enzymolysis: adding an activated compound enzyme preparation into a product obtained in the step (1) for enzymolysis, meanwhile, carrying out intermittent ultrasonic treatment in the enzymolysis process, and circulating the operation; and (3) enzyme deactivation: carrying out enzyme deactivation on the product obtained in the step (2), and then carrying out freezedrying to obtain the full-enzyme polypeptide for the fertilizer. The full-enzyme polypeptide for the fertilizer has better physiological activity, permeability and stability, is easily absorbed by crops, is not easily inactivated by environmental influence, has low chelating process conditions, and can chelate metal ions with different chemical activities. The polypeptide disclosed by the invention further has the capability of inhibiting harmful bacteria in soil, and is wide in antibacterial spectrum.

Description

technical field [0001] The invention relates to the technical field of polypeptide production, in particular to a production process of holoenzyme polypeptide for fertilizer. Background technique [0002] Polypeptides are divided into two types: biologically active peptides and artificially synthesized peptides. Bioactive peptides are mainly obtained from proteins through biological extraction and have strong activity. In terms of agricultural applications, polypeptides can not only provide organic nutrients for crops, but also have chelating properties. It can chelate with medium elements to form chelated compounds with good water solubility. The physiological activity of polypeptides allows crops to directly absorb amino acid polypeptides when using the chelated metal elements. Polypeptide fertilizers are as effective as urea fertilizers. , can also improve the microbial community structure in the soil and increase the chlorophyll content of plants, but the production cost...

AI Technical Summary

Problems solved by technology

[0002] Polypeptides are divided into two types: biologically active peptides and artificially synthesized peptides. Bioactive peptides are mainly obtained from proteins through biological extraction and have strong activity. In terms of agricultural applications, polypeptides can not only provide organic nutrients for crops, but also have chelating properties. It can chelate with medium elements to form chelated compounds with good water solubility. The physiological activity of polypeptides allows crops to directly absorb amino acid polypeptides when using the chelated metal elements. Polypeptide fertilizers are as effective as urea fertilizers. , can also improve the microbial community structure in the soil and increase the chlorophyll content of plants, but the production cost is high, and the traditional enzymatic protein hydrolysis method makes the protein hydrolysis rate low, the chelation stability is poor, and it is easily destroyed or absorbed by harmful microorganisms in the soil. This leads to loss of nutrients and increased disease

[0003] Patent No. CN201710254558.6 discloses a calcium-containing water-soluble compound fertilizer and its preparation process. It uses soybean meal as the main component, supplemented with potatoes, yams, tea meal, molasses and sodium chloride to prepare a solid fermentation medium. Fermentation of Lactobacillus , Bacillus subtilis, Bacillus subtilis subsp. subsp. and other multi-bacteria combined step-by-step fermentation to prepare plant active peptides, but this fertilizer does not have antibacterial effect, so the ability to inhibit harmful bacteria pollution is not ideal, and the fermentation time is longer

[0004] Patent No. CN201510944758.5 ​​discloses the preparation method of bioactive polypeptide calcium fertilizer, which overcomes the problems that calcium hydroxide is easy to form calcium carbonate precipitation with carbon dioxide in the air, which is not easy to store, the prepared product does not have biological activity, and the production increase effect is not significant. , but the use of polysuccinimide is irritating, and it should avoid inhalation and contact with the skin when using it, which leads to stricter production safety and weaker operability

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