Preparation method and application of ratiometric fluorescent probe for detecting copper ions

A ratio fluorescent probe, copper ion technology, applied in the field of fluorescence sensing, can solve the problems of fluorescence intensity change signal instrument accuracy and stability of fluorescent probe concentration interference, etc., to eliminate light source fluctuation, high sensitivity, excellent visualization effect. Effect

Pending Publication Date: 2020-04-21
ZHEJIANG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the fluorescence sensing analysis based on quantum dots, a single fluorescence intensity change signal is ea...

Method used

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  • Preparation method and application of ratiometric fluorescent probe for detecting copper ions
  • Preparation method and application of ratiometric fluorescent probe for detecting copper ions
  • Preparation method and application of ratiometric fluorescent probe for detecting copper ions

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[0026] The preparation method of a carbon quantum dot / glutathione-modified cadmium telluride quantum dot CQDs / GSH@CdTe QDs ratio fluorescent probe for detecting copper ions provided by the invention, the specific steps are as follows:

[0027] 1) Synthesis of carbon quantum dots CQDs: citric acid and ethylenediamine with a mass volume ratio (g:mL) of 1:1 to 3, hydrothermally reacted at 150°C to 200°C for 6 to 18h, the hydrothermal reaction temperature Preferably at 180°C, the reaction time is preferably 6h, under the condition that the centrifugation time is 15min and the centrifugation speed is 4000rpm, the reaction product is centrifuged and dialyzed to obtain CQDs, wherein the volume ratio of ethylenediamine and water is 1:20, and then Dilute 10 with ultrapure water 4 Store in a refrigerator at 4°C after doubling for later use;

[0028] 2) Synthesis of glutathione-modified cadmium telluride quantum dots GSH@CdTe QDs: Cadmium chloride hydrate CdCl 3 2.5H 2 O was dissolved...

Embodiment 1

[0039] This embodiment provides the preparation method of CQDs / GSH@CdTe QDs ratio fluorescent probe, the specific steps include:

[0040] Step 1. Synthesis of carbon quantum dots: Add 1mL of ethylenediamine and 500mg of citric acid into the reaction kettle, hydrothermally react in 20ml of water at 180°C for 6h, after the product is cooled, centrifuge at 4000rpm for 15min, then take the supernatant, Filter with a 0.22 μm filter membrane, then transfer the filtered solution into a dialysis bag with a molecular weight of 3500Da for dialysis for 24 hours, and finally dilute the purified carbon quantum dots for 10 4 Store in a 4°C refrigerator for later use.

[0041] Step 2, synthesis of glutathione-modified cadmium telluride quantum dots: 0.2923g cadmium chloride hydrate CdCl 3 2.5H 2 O was dissolved in 100 mL of water, and 0.8 g of trisodium citrate dihydrate (C 6 h s Na 3 o 7 .2H 2 O), then add 0.4g glutathione (GSH), 0.0177g sodium tellurite (Na 2 TeO 3 ) and 0.4g sodi...

Embodiment 2

[0044] This embodiment provides the preparation method of CQDs / GSH@CdTe QDs ratio fluorescent probe, the specific steps include:

[0045] Step 1. Synthesis of carbon quantum dots: Add 0.5mL ethylenediamine and 500mg citric acid into the reaction kettle, hydrothermally react in 10ml water at 150°C for 18h, cool the product and centrifuge at 4000rpm for 15min, then take the supernatant, Filter with a 0.22 μm filter membrane, then transfer the filtered solution into a dialysis bag with a molecular weight of 3500Da for dialysis for 24 hours, and finally dilute the purified carbon quantum dots for 10 4 Store in a 4°C refrigerator for later use.

[0046] Step 2, synthesis of glutathione-modified cadmium telluride quantum dots: 0.1462g cadmium chloride hydrate CdCl 3 2.5H 2 O was dissolved in 100 mL of water, and 0.4 g of trisodium citrate dihydrate (C 6 h s Na 3 o 7 .2H 2 O), then add 0.2g glutathione (GSH), 0.0089g sodium tellurite (Na 2 TeO 3 ) and 0.2g sodium borohydride...

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Abstract

The invention discloses a preparation method and application of a ratiometric fluorescent probe for detecting copper ions. The method specifically comprises the following steps: firstly, preparing water-soluble CQDs from ethylenediamine and citric acid by virtue of a one-step hydrothermal method, and taking the water-soluble CQDs as reference fluorophores; then, through a reflux method, synthesizing GSH@CdTe QDs adopted as detection fluorophores of the ratiometric fluorescent probe, with the molar ratio of cadmium chloride hydrate, sodium tellurite, glutathione, trisodium citrate dihydrate tosodium borohydride in the reaction being controlled to be 1:0.06:1.02:2.13:8.28; finally, directly mixing the CQDs and the GSH@CdTe QDs to prepare the CQDs/GSH@CdTe QDs ratiometric fluorescent probe.Under the optimal condition, the prepared ratiometric fluorescent probe is used for quantitatively detecting copper ions based on a fluorescence quenching method. The detection limit of the ratiometric fluorescent probe to copper ions is as low as 0.35 nM, and the ratiometric fluorescent probe has great application potential in copper ion detection and analysis.

Description

technical field [0001] The invention belongs to the technical field of fluorescence sensing, and in particular relates to a method for preparing a ratio fluorescent probe for detecting copper ions and an application thereof. Background technique [0002] Copper is an essential trace element for biological growth, but excessive copper will cause serious damage to organisms. Due to the characteristics of high accumulation, easy migration, and persistence of copper ions, the illegal discharge of copper-containing waste in industry and agriculture has caused excessive copper content in water and soil. At present, there are many classic methods for heavy metal detection, such as atomic absorption spectrometry (AAS), atomic emission spectrometry (AES), cold atomic fluorescence spectrometry (CV-AFS), inductively coupled plasma-mass spectrometry (ICPMS) and mass spectrometry. (MS) and other detection methods. These methods have the advantages of high sensitivity and good selectivi...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/88G01N21/64
CPCC09K11/06C09K11/883G01N21/643G01N21/6447G01N2021/6432
Inventor 佘远斌孟清君付海燕胡瑛
Owner ZHEJIANG UNIV OF TECH
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