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Biological agent for relieving cadmium stress of leaf vegetable planting soil and application of biological agent

A technology of biological agents and leafy vegetables, which is applied in the field of biological agents to alleviate cadmium stress in leafy vegetable planting soil, to achieve the effect of alleviating physiological inhibition, high environmental friendliness, and not easy to secondary damage

Active Publication Date: 2020-05-05
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the present situation of soil cadmium pollution and selenium application, the problem to be solved in the present invention is to provide a biological agent and its application for alleviating the cadmium stress in leafy vegetable planting soil.

Method used

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  • Biological agent for relieving cadmium stress of leaf vegetable planting soil and application of biological agent
  • Biological agent for relieving cadmium stress of leaf vegetable planting soil and application of biological agent
  • Biological agent for relieving cadmium stress of leaf vegetable planting soil and application of biological agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A biological agent and application thereof for alleviating cadmium stress in leafy vegetable planting soil, the specific steps are as follows:

[0045] (1) Added 400mmol L after sterilization -1 In the LB culture medium of sodium selenite, inoculate 10% live bacteria body of Bacillus subtilis 168 according to the volume ratio, and then place the culture bottle in a shaking flask incubator at 30°C under the condition of 150 rpm. Incubate with oxygen for 24 hours, then centrifuge at 4000g for 10 minutes, collect the precipitate to obtain a mixture of Bacillus subtilis and its metabolite nano-selenium;

[0046] Wherein, the formula of LB (Luria-Bertani) is: 10g / L tryptone, 5g / L yeast extract and 10g / L sodium chloride, adjust the pH value to 7.0-7.5, and sterilize.

[0047] (2) Wash the resulting precipitate with 0.9% NaCl solution, then add liquid nitrogen and beat it with a pestle to break the precipitate; then ultrasonically treat it at 200W for 5 minutes to make the pa...

Embodiment 2

[0054] A biological agent and application thereof for alleviating cadmium stress in leafy vegetable planting soil, the specific steps are as follows:

[0055] (1) Add 500mmol L after sterilization -1 In the LB culture medium of sodium selenite, inoculate 6% of Bacillus subtilis 168 living thalline according to the volume ratio, then place the culture bottle in a shake flask incubator at 32°C under the condition of 200 rev / min. Incubate with oxygen for 14 hours, then centrifuge at 4500g for 15 minutes, collect the precipitate to obtain a mixture of Bacillus subtilis and its metabolite nano-selenium;

[0056] (2) Wash the resulting precipitate with 0.9% NaCl solution, then add liquid nitrogen and beat it with a pestle to break the precipitate; then ultrasonically treat it at 100W for 10 minutes to make the particle size of the precipitated mixture below 200nm, and use 1.5mol L -1 1. The Tris-HCl buffer solution with pH 8.3 was continuously centrifuged at 10,000 g for 5 minutes ...

Embodiment 3

[0062] The biological preparation prepared by using nano-selenium prepared by Bacillus subtilis 168 biological method according to the present invention is compared with the biological preparation prepared by commercially available nano-selenium in alleviating the effect of cadmium stress in leafy vegetable planting soil

[0063](1) 500 mL fermented nano-selenium nutrient solution (250 mg / L selenium content) was purchased from Dongming Wheat Cooperative, and 133.3 g Bacillus subtilis was added to prepare biological preparations.

[0064] (2) Rapeseed seedlings were raised for two weeks in the early stage, and then transferred to a hydroponic device. Slow the seedlings with 1 / 2 Hoagland nutrient solution for three days, then start to add cadmium-containing solution (50 μmol L -1 ). Afterwards, 8 days after the growth of rape seedlings, the leaves of rapeseed were evenly sprayed for the first time, specifically, 5, 10, 15, and 25 mg / L nano-selenium containing 26.7 mg / L Bacillus...

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Abstract

The invention discloses a biological agent for relieving cadmium stress of leaf vegetable planting soil. The biological agent is prepared by uniformly mixing nano-selenium prepared by a bacillus subtilis 168 biological method with bacillus subtilis with the viable count more than or equal to 1* 10 <9> / g in water. The invention also discloses application of the biological agent in relieving the cadmium stress of the leaf vegetable planting soil. Experiments prove that the biological agent can supplement selenium element for plants, prevent and control plant diseases and insect pests and remarkably inhibit migration of cadmium from the underground part to the overground part when being sprayed on leaf surfaces, and plays an important role in repairing a Cd-polluted soil-microorganism-plant ecological system. Meanwhile, the biological agent is high in environmental friendliness, does not easily cause secondary pollution of the soil and secondary damage to the plants, and has a great application prospect in the aspect of agricultural application.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a biological preparation for alleviating cadmium stress in leaf vegetable planting soil and an application thereof. Background technique [0002] In recent years, heavy metal pollution has become a global environmental problem. The 2014 "National Soil Pollution Survey Bulletin" shows that the rate of cadmium (Cd) exceeding the standard ranks first among heavy metal pollutants, which has aroused widespread concern. Due to mining and smelting, agricultural sewage irrigation and application of pesticides and fertilizers, a large amount of cadmium is released into the soil, which aggravates the problem of soil cadmium pollution. On the one hand, cadmium will destroy the chloroplast structure of plants, cause photosynthesis to be blocked, reduce plant metabolism, and seriously inhibit the growth of plants, especially leafy vegetables. On the other hand, c...

Claims

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Application Information

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IPC IPC(8): A01G22/15A01G7/06A01G13/00C12N1/20C12R1/125
CPCA01G7/06A01G13/00A01G22/15C12N1/20
Inventor 王曙光亓文钰宋超
Owner SHANDONG UNIV
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