Complex microbial inoculant for cardiovascular diseases

A compound bacterial agent and cardiovascular technology, applied in the field of microorganisms, can solve problems such as increasing the risk of cardiovascular diseases, thrombus blockage, narrowing of arterial diameter, etc., and achieve the effects of improving immunity, improving health, and ensuring safety

Active Publication Date: 2020-06-16
成都六然医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the plaque thickens, the diameter of the artery will narrow or even be blocked by a thrombus, increasing the risk of cardiovascular disease

Method used

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  • Complex microbial inoculant for cardiovascular diseases
  • Complex microbial inoculant for cardiovascular diseases
  • Complex microbial inoculant for cardiovascular diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Determination of total cholesterol content in the serum of embodiment 1

[0035] 1. Activated bacteria:

[0036] Lactobacillus plantarum CGMCC №.3698: Take a ring of Lactobacillus plantarum and inoculate it into MRS solid medium for streaking, and cultivate anaerobically at 37°C for 24 hours. Pick a single colony with good growth in the plate and inoculate it into MRS liquid medium for activation, and culture it anaerobically to 0.5*10 8 cfu / ml of Lactobacillus plantarum CGMCC №.3698 seed liquid, adjusted to pH 6.0 with lactic acid.

[0037] 2. Configure liquid

[0038] Preparation of the Chiatin A solution: use water, Tween 80 (mass fraction 1%) and Chiatin A to prepare a solution with a concentration of Chiatin A of 10 mg / kg.

[0039] 3. Fermentation broth preparation

[0040] a. Lactobacillus plantarum CGMCC №.3698 fermentation liquid: Lactobacillus plantarum CGMCC №.3698 seed liquid is activated in MRS liquid medium, anaerobic culture is 24h, and the use medium ...

Embodiment 2

[0065] Embodiment 2 triglyceride determination

[0066] 1. Determination of triglyceride content in serum

[0067] Determination method: configure blank tube (distilled water 10ul, working solution 1000ul), calibration tube (2.26mmol / L calibrator 10ul, working solution 1000ul), sample tube solution (sample 10ul, working solution 1000ul). Mix well, incubate at 37°C for 10min, wavelength 510nm, optical path 0.5cm, adjust to zero with distilled water, and measure the absorbance value of each tube.

[0068] Calculation formula: Triglyceride content (nmol / L) = (sample OD value - blank OD value) / (calibration OD value - blank OD value) * calibrator concentration

[0069] According to the calculation formula, the triglyceride content of rat samples in different groups was determined.

[0070] 2. Statistical analysis was performed using SPSS16.0, and the results of measurement data were expressed as mean ± standard deviation, and the t-test between groups was used.

[0071] 3. The...

Embodiment 3

[0079] Stability test: After adding red yeast rice powder, natto freeze-dried powder, and chitosan to the Lactobacillus plantarum + Chitosin A treatment group, the lipid-lowering effect did not decrease after the test, and it could be stabilized after homogeneous emulsification Stable at room temperature for 12 months.

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Abstract

The invention discloses a complex microbial inoculant for cardiovascular diseases, which comprises lactobacillus plantarum CGMCC No. 3698 and other necessary auxiliary agents, and can also comprise red yeast rice powder, natto freeze-dried powder and chitosan. The complex microbial inoculant can also comprise other probiotics, such as lactobacillus casei and lactobacillus paracasei. Preferably, the complex microbial inoculant further comprises coriolus versicolor element A. The complex microbial inoculant can be prepared into oral liquid, freeze-dried powder, capsules and the like, and is preferably used as the oral liquid.

Description

technical field [0001] The invention relates to a composite bacterial agent for cardiovascular, belonging to the technical field of microorganisms. Background technique [0002] Cardiovascular and cerebrovascular diseases are collectively referred to as cardiovascular and cerebrovascular diseases. disease. Cardiovascular and cerebrovascular disease is a common disease that seriously threatens the health of human beings, especially middle-aged and elderly people over 50 years old. It has the characteristics of high prevalence, high disability rate and high mortality rate. However, there are still more than 50% of cerebrovascular accident survivors who cannot take care of themselves completely. The number of cardiovascular and cerebrovascular diseases in the world is as high as 15 million people every year, ranking first among various causes of death. [0003] Dyslipidemia can lead to a variety of diseases, the most serious of which is cardiovascular disease. Cardiovascular...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61K36/899A61K36/48A61K31/365A61K9/08A61K9/19A61K9/48A61P3/06A61P9/00A23L33/135A23L31/00A23L33/21A23L33/10A23C9/123A23K10/18A23K10/12A23K20/163A61K31/722
CPCA61K35/747A61K31/365A61K36/899A61K36/062A61K36/48A61K31/722A61K9/0095A61K9/19A61K9/48A61P3/06A61P9/00A23L33/135A23L31/00A23L33/21A23L33/10A23C9/123A23K10/18A23K10/12A23K20/163A61K2236/19A23V2002/00A23V2400/125A23V2400/165A23V2400/169A61K2300/00A23V2200/3262A23V2200/326A23V2200/30A23V2250/5116A23V2250/511
Inventor 赵一鸣
Owner 成都六然医疗科技有限公司
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