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Fucoxanthin-containing algal oil and extraction method thereof, and healthy food

A technology of fucoxanthin and an extraction method is applied in the directions of food ingredients, food science, fat oil/fat production, etc., and can solve the problems of high extraction cost of fucoxanthin, low content of fucoxanthin, unsatisfactory effect and the like, Achieve the effect of reducing the extraction cost, increasing the fucoxanthin content, and being beneficial to recovery and reuse

Pending Publication Date: 2020-06-16
GUOTOU BIO TECH INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually methanol, ethanol, acetone or ethyl acetate are used for extraction alone or mixed with several solvents, and the effect of extracting fucoxanthin in microalgae is not ideal, mainly manifested as fucoxanthin in the extract The content of fucoxanthin is very low and there are many impurities, which can not be directly used as food additives, but also brings great difficulties to the subsequent further purification, resulting in the high cost of comprehensive extraction of fucoxanthin

Method used

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  • Fucoxanthin-containing algal oil and extraction method thereof, and healthy food
  • Fucoxanthin-containing algal oil and extraction method thereof, and healthy food
  • Fucoxanthin-containing algal oil and extraction method thereof, and healthy food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] First step extraction

[0069] The algal biomass containing fucoxanthin is the dry algae powder of Phaeodactylum tricornutum. Weigh a certain amount of Phaeodactylum tricornutum algae powder into a conical reaction tube. Add 1g Phaeodactylum tricornutum powder to 6mL of the first organic Add n-hexane to the ratio of the solvent, vortex to mix uniformly, place in a water bath at 25°C and stir for 30 min, then centrifuge at 3000 rpm for 5 min. The extraction was repeated once under the same conditions, and the two supernatants were combined, and the n-hexane was removed by a nitrogen blower, and dried in a vacuum drying oven at room temperature to obtain the first step extraction extract. The raffinate phase is used for the second leaching step.

[0070] The second step of extraction

[0071] Add ethyl acetate according to the ratio of adding 6 mL of the second organic solvent to 1 g of the raffinate phase, vortex to mix evenly, place in a 25°C water bath for stirring and extr...

Embodiment 2

[0075] Phaeodactylum tricornutum was leached according to the method of Example 1 according to the experimental conditions described in Table 1 below to obtain the first-step leaching extract and the second-step leaching extract. The components of the first step extract and the second step extract were analyzed by gas chromatography-mass spectrometry (GC-MS). The results are shown in Table 2. Samples 1-3 use Phaeodactylum tricornutum dry algae powder, samples 4-6 use Phaeodactylum tricornutum algae mud (the water content of the algae mud is 60%)

[0076]

Embodiment 3

[0078] The algal biomass containing fucoxanthin is Phaeodactylum tricornutum algal mud (the water content of the algal mud is 80%), and a certain amount of Phaeodactylum tricornutum algal mud is weighed into a cone-shaped reaction tube. Add n-hexane according to the ratio of 1g Phaeodactylum tricornutum to 6mL of the first organic solvent, vortex to mix evenly, place it in a 25°C water bath, stir and extract for 30min, then centrifuge at 3000rpm for 5min. The extraction was repeated once under the same conditions, and the two supernatants were combined, and the n-hexane was removed by a nitrogen blower, and dried in a vacuum drying oven at room temperature to obtain the first step extraction extract. The raffinate phase is leached in the second step.

[0079] The second step of extraction

[0080] Add ethyl acetate according to the ratio of adding 6 mL of the second organic solvent to 1 g of the raffinate phase, vortex to mix uniformly, place it in a water bath at 25° C., stir and...

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Abstract

The invention relates to an extraction method of fucoxanthin, and discloses an extraction method of fucoxanthin-containing algal oil. The method comprises the following steps: S1, carrying out first-step extraction on fucoxanthin-containing algal biomass by using a first organic solvent, and carrying out solid-liquid separation to obtain a raffinate phase; and S2, carrying out second-step extraction on the raffinate phase separated in the step S1 by using a second organic solvent, and carrying out solid-liquid separation to obtain fucoxanthin-containing algal oil, wherein the dielectric constant of the first organic solvent in the normal state is less than or equal to 2 F / m, and the dielectric constant of the second organic solvent in the normal state is 6-20 F / m. According to the extraction method disclosed by the invention, the contents of fucoxanthin and EPA in the extract is greatly increased, and the content of impurities in the extract is reduced; and the fucoxanthin-containing algal oil can be used as a raw material of a fucoxanthin-rich food or health care product after being simply concentrated, so that the fucoxanthin extraction process is simplified, and the extraction cost is reduced.

Description

Technical field [0001] The invention relates to a method for extracting fucoxanthin, in particular to a fucoxanthin-containing algal oil, an extraction method thereof, and a health food containing the fucoxanthin-containing algal oil. Background technique [0002] Fucoxanthin, also known as fucoxanthin or fucoxanthin, is an oxygen-containing derivative of carotene. It is a tasteless, orange-red or reddish-brown powdery substance, mainly derived from large seaweeds-brown algae, diatoms, gold Species of microalgae, such as Algae and Pseudomonas, whose molecular formula is C 42 H 58 O 6 , The molecular weight is 658.91, the density is 1.09g / mL, and the melting point is 166-168°C. Fucoxanthin is a fat-soluble pigment, insoluble in water, and easily soluble in organic solvents such as ethanol, acetone, n-hexane and chloroform. Because fucoxanthin has a unique molecular structure, such as a polyolefin skeleton (belonging to allene-type carotenoids), 5,6-monoepoxy, 9-conjugated double ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10A23L33/12
CPCC11B1/10A23L33/12A23V2002/00A23V2250/186
Inventor 胡强喻正保加晶楚平广
Owner GUOTOU BIO TECH INVESTMENT CO LTD