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Preparation method for corn polypeptide-selenium chelate

A corn polypeptide and chelate technology, which is applied in the field of food processing, can solve the problems of poor water solubility, limiting protein dissolution in corn meal, and reducing protein extraction rate and protein purity.

Inactive Publication Date: 2020-07-07
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Utilize biotechnology to carry out deep processing of corn protein resources, and then use inorganic selenium for chelation to prepare polypeptide-selenium chelate, and transform inorganic selenium into organic selenium bioactive polypeptide-selenium chelate can significantly improve the absorption rate of selenium and utilization rate, the following problems still exist in the technology disclosed in the prior art at present: (1) if the oil content in the raw material is too much, it will cause serious emulsification when the protein is extracted in the follow-up, which greatly limits the amount of oil in the corn meal. The dissolution of protein leads to a decrease in protein extraction rate and protein purity, thereby correspondingly reducing the conversion rate of protein polypeptide; (2) due to the high proportion of non-polar amino acid residues in zein, resulting in poor water solubility, zein After converting to polypeptide, although the problem of poor solubility can be improved, the solubility still needs to be improved

Method used

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  • Preparation method for corn polypeptide-selenium chelate

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Effect test

Embodiment 1

[0038] A preparation method of corn polypeptide-selenium chelate, comprising the following steps:

[0039] (1) Huajian No. 1 dry corn is ground first to obtain Huajian No. 1 corn coarse grains, and then the Huajian No. 1 corn coarse grains are degreased by hydraulic cold pressing to obtain corn meal, and the corn meal is crushed, After passing through a 60-mesh sieve, add distilled water to the corn meal according to the ratio of material to liquid ratio of 1:3. Ultrasonic treatment for 50 minutes, wherein the frequency of the first ultrasonic treatment is 150W, and the temperature is 28°C. After the first ultrasonic treatment is completed, the first fermentation treatment is carried out at 28°C for 6 hours to obtain the first fermentation material; , the oil-degrading bacterium is a composite strain composed of Pseudomonas monsonii and Candida crassa, and the weight ratio of the Pseudomonas monsonii and Candida crassa is 1:1.5.

[0040] (2) After the first fermented material...

Embodiment 2

[0045] A preparation method of corn polypeptide-selenium chelate, comprising the following steps:

[0046] (1) Huajian No. 1 dry corn is ground first to obtain Huajian No. 1 corn coarse grains, and then the Huajian No. 1 corn coarse grains are degreased by hydraulic cold pressing to obtain corn meal, and the corn meal is crushed, After passing through a 60-mesh sieve, add distilled water to the obtained under-sieve according to the ratio of material to liquid ratio of 1:5, after beating, add oil-degrading bacteria with 4% of the weight of corn meal by weight percentage, and carry out the first time after stirring well. Ultrasonic treatment for 55 minutes, wherein the frequency of the first ultrasonic treatment is 220W, and the temperature is 30°C. After the first ultrasonic treatment is completed, the first fermentation treatment is carried out at 30°C for 9 hours to obtain the first fermentation material; , the oil-degrading bacterium is a composite strain composed of Pseudom...

Embodiment 3

[0052] A preparation method of corn polypeptide-selenium chelate, comprising the following steps:

[0053] (1) Huajian No. 1 dry corn is ground first to obtain Huajian No. 1 corn coarse grains, and then the Huajian No. 1 corn coarse grains are degreased by hydraulic cold pressing to obtain corn meal, and the corn meal is crushed, After passing through a 60-mesh sieve, add distilled water to the obtained under-sieve according to the ratio of material to liquid ratio of 1:6. After beating, add oil-degrading bacteria with 6% of the weight of corn meal by weight percentage, and carry out the first time after stirring well. Ultrasonic treatment for 60 minutes, wherein the frequency of the first ultrasonic treatment was 300W, and the temperature was 38°C. After the first ultrasonic treatment was completed, the first fermentation treatment was carried out at 38°C for 12 hours to obtain the first fermentation material; , the oil-degrading bacteria is a composite strain composed of Pse...

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Abstract

The invention belongs to the technical field of food processing, and particularly relates to a preparation method for corn polypeptide-selenium chelate. The preparation method comprises the followingsteps of: (1) after a Huajian No.1 dry corn is firstly smashed, carrying out degreasing processing, and then, adopting grease degradation bacteria to carry out first-time fermentation to obtain a first fermentation material; (2) carrying out enzymolysis on the first fermentation material by a composite enzyme to obtain an enzymolysis solution; (3) adding brevibacterium lactofermentum and stenotrophomonas maltophilia into the enzymolysis solution at a certain ratio to carry out second-time fermentation to obtain a second fermentation material; (4) adding selenium-enriched yeast into the secondfermentation material to carry out selenium chelation processing to obtain a selenium chelation material; and (5) and carrying out pre-freezing, vacuum freezing drying, packaging and sterilization. According to the preparation method disclosed by the invention, a selenium element can be provided for the human body, a grease content is further lowered in a preparation process, so that a protectionextraction rate and purity and a protein polypeptide conversion rate are correspondingly improved, and product solubleness is improved.

Description

【Technical field】 [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method of corn polypeptide-selenium chelate. 【Background technique】 [0002] Ultra-high-oil and high-protein corn "Huajian No. 1" (Gui Shenyu 2011017) is a new type of corn variety created by Nanning Guifuyuan Agriculture Co., Ltd. It is characterized by a grain protein content of up to 24% at the mature stage of corn seeds, and oily The rate is 27%. It belongs to high-oil and high-protein grains and is a non-genetically modified crop. Corn gluten meal is also called corn gluten meal, and its main chemical component is protein, usually more than 70%. The reuse of corn gluten powder will not only produce good economic benefits, but also adapt to the overall planning of energy saving and emission reduction and building a conservation-oriented society. The development of corn peptides makes corn gluten powder from insoluble to soluble products. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P21/06C07K1/107C12R1/13C12R1/01
CPCC07K1/107C12P21/00C12P21/06
Inventor 李明娟游向荣张雅媛王颖卫萍周葵黄展文
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI