Convenient method and device for efficiently enriching N-DAMO bacteria
An enrichment and high-efficiency technology, which is applied in the fields of biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc. Easy to obtain, easy to assemble effect
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specific Embodiment approach 1
[0030] Assemble the device enriched with N-DAMO bacteria, such as figure 1 shown.
[0031] The first stage: Take 100g of wetland soil at a depth of 40-60cm in the nature reserve and place it in the reactor glass bottle 9, add 0.6L of liquid basal medium, and add NaNO 2 Make its concentration in liquid basal medium 1mmol / L. Use 1mol / L HCl to adjust the pH value to 7.0-7.5, open the stop valve of the ventilation pipe 1, charge high-purity (99.99%) methane gas, close the stop valve of the ventilation pipe 1 after 5 minutes, seal the whole system, and place In an air bath shaker, at 30 °C, methane partial pressure (P CH4 ) under the condition of 101kPa for continuous culture, after 24h, NO 2 - Consumption is exhausted, the reaction is over, and the precipitation is allowed to stand still.
[0032] The second stage: open the stop valve on the peristaltic pump 5 and the water change pipe 3, discharge the supernatant of 0.4L in the reactor glass bottle 9 into the liquid storage ...
specific Embodiment approach 2
[0037] Investigate the environmental adaptability of N-DAMO bacteria under different pH, temperature and NaCl concentration conditions.
[0038] Scheme one: different from the specific embodiment one, the liquid basal medium under different pH conditions is enriched with N-DAMO (using the HCl solution of 1mol / L or the NaOH solution of 1mol / L to adjust the pH of the liquid basal medium value). A pH gradient of 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0 was investigated. The comparison shows that the enrichment of N-DAMO bacteria is more conducive to the enrichment of N-DAMO bacteria under the condition of pH 7-8, and N-DAMO bacteria show good metabolic activity.
[0039] Solution 2: The difference from the specific embodiment 1 is that N-DAMO is enriched under different temperature conditions. The temperature gradients investigated were 20°C, 25°C, 30°C, 35°C, 40°C and 45°C. The comparison shows that the temperature between 30°C and 40°C is more conducive to the enrichme...
specific Embodiment approach 3
[0042] Growth factors (vitamins, heme and betaine) can further improve the metabolic growth ability of N-DAMO bacteria.
[0043] Scheme 4: Different from Embodiment 1, vitamin solution is added to the liquid basal culture, and the concentrations of the vitamin solution in the liquid basal medium are 0mL / L, 0.01mL / L, 0.02mL / L, 0.03mL / L, respectively. L, 0.05mL / L, 0.07mL / L and 0.10mL / L. The comparison shows that the addition of vitamin solution can significantly improve the growth and metabolic activity of DAMO bacteria. When the vitamin solution is 0.02mL / L, it is more conducive to the enrichment of N-DAMO bacteria, and N-DAMO bacteria show good metabolic activity. Among them, 1L vitamin solution contains 2.00g biotin, 5.00g thiamine hydrochloride, 5.00g D-Ca-pantothenic acid, 5.00g lipoic acid, 2.00g folic acid, 5.00g riboflavin, 0.10g microbial B12, 10.00g vitamin B6, 5.00g niacin and 5.00g p-aminobenzoic acid.
[0044] Scheme 5: The difference from Embodiment 1 is that hem...
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