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Chinese wolfberry endophytic fusarium nematophilum strain and application thereof

A technology of Fusarium verticillum and strains, applied in the field of plant endophytic fungi and its applications, can solve the problems of temperature regulation and control of fruit chilling damage, health threats, and excessive pesticide residues, so as to prolong the freshness period of fruits, reduce the rate of decay, and better control The effect of bacterial activity

Active Publication Date: 2020-07-24
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost of low-temperature storage is high, the energy consumption is high, and improper temperature control can easily cause chilling damage to the fruit, and the quality of the fruit is unstable; chemical fungicide preservation has problems such as fruit pollution, excessive pesticide residues, health threats, and increased drug resistance of pathogenic bacteria. boycotted by consumers

Method used

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  • Chinese wolfberry endophytic fusarium nematophilum strain and application thereof
  • Chinese wolfberry endophytic fusarium nematophilum strain and application thereof
  • Chinese wolfberry endophytic fusarium nematophilum strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Confrontation culture: using Alternaria A.alternata and Botrytis cinerea B.cinerea as target saprophytic fungi of Lingwu long jujube, through confrontation culture in a dish, the inhibitory effect of endogenous Fusarium verticularis strain NQ8GII4 on saprophytic fungi was determined . An endogenous Fusarium verticillium NQ8GII4 bacterium cake with a diameter of 6 mm was inoculated at one end 2.5 cm from the center of the PDA plate, and a rot-causing pathogen bacterium cake with a diameter of 6 mm was inoculated at the other end, sealed with a parafilm and placed in a mold incubator at 25°C for cultivation. Simultaneous inoculation of endophytic Fusarium verticillium and rot-causing pathogen, inoculation of endophytic Fusarium verticularis and rot-causing pathogen 4 days after inoculation, and inoculation of endophytic fungus after inoculation of rot-causing pathogen 4 days, each treatment was repeated 4 times, and only inoculated The flat plate of the rot-causing pathog...

Embodiment 2

[0050] Microscopic observation of antagonism:

[0051] The cover slip culture method was adopted. Endogenous Fusarium verticillium NQ8GII4 with a diameter of 6 mm and rot-causing pathogenic bacteria cakes were respectively inoculated at the end 1.5 cm away from the center of the PDA flat plate, and a sterilized cover glass soaked in PDA medium was placed between the two bacterial cakes, and the endophytic fungi and pathogenic bacteria were treated. After 1-3 days of contact with the hyphae of rot pathogenic bacteria, the cover glass was taken out, observed and photographed under an optical microscope (400 times).

[0052] figure 2 It is the result of microscopic observation of the interaction between endophytic Fusarium verticillium NQ8GII4 hyphae, Alternaria alternata and Botrytis cinerea mycelia. The hyphae of Alternaria alternata and Botrytis cinerea growing vigorously on the PDA medium, with uniform thickness, more sporulation, and uniform spore size ( figure 2 A and ...

Embodiment 3

[0054] Determination of antibacterial activity of endophytic Fusarium verticillium NQ8GII4 culture filtrate against rot-causing pathogens:

[0055] Preparation of the culture filtrate of endophytic Fusarium verticillium NQ8GII4: Take 6 pieces of fungus cakes with a diameter of 6mm from pre-cultivated Fusarium verticillium NQ8GII4 for 5 days, put them into a conical flask containing 150mL of PDB medium, and keep at 25°C for 175r / min cultured for 7d to obtain fermentation broth (2.3×10 7 CFU / mL). First use a suction filter pump to filter out the mycelium and spores of the fermentation broth, and then filter through a 0.22 μm microporous membrane for 3 times to sterilize to obtain a culture filtrate, which is stored at 4°C for later use.

[0056] Inhibitory effect of endogenous Fusarium verticillium NQ8GII4 culture filtrate on mycelial growth of rot-causing pathogens: mix the culture filtrate of Fusarium verticulum NQ8GII4 with sterilized PDA medium cooled to about 50°C, and pr...

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Abstract

The invention discloses a Chinese wolfberry endophytic fungus strain and an application thereof, and belongs to the technical field of bioengineering. The invention provides a Chinese wolfberry endophytic fusarium nematophilum strain NQ8GII4 and an application thereof, wherein the preservation number is CGMCC No.19271; the culture filtrate of the endophytic fungus can obviously inhibit the growthof a rot-causing pathogenic bacteria, and the endophytic fungus has very high bacteriostatic activity, and the strain can enable pathogenic bacteria hyphae to have the phenomena of malformation, disintegration and spore wizening, has an obvious antagonistic effect on rot-causing pathogenic bacteria, and has the characteristic of antagonism broad spectrum, and volatile substances generated by the strain can obviously inhibit the growth of the pathogenic bacteria hyphae. The invention further provides a preparation method and a use method of a biological fresh-keeping agent containing the Chinese wolfberry endophytic fusarium nematophilum strain NQ8GII4 culture filtrate component, and the biological fresh-keeping agent prepared from the strain culture filtrate can significantly reduce the rotting rate of fruits in the storage period, and prolong the shelf life of fruit foods, and wide application prospects are realized in the aspect of postharvest fresh keeping of fruits.

Description

technical field [0001] The invention relates to a plant endophytic fungus and its application, in particular to a wolfberry endophytic Fusarium verticularis strain NQ8GII4 and its application. Background technique [0002] Plant endophytic fungi refer to a class of fungi that live in host plant tissues during a certain stage or the entire stage of their life history, but do not cause obvious disease symptoms to the host plant. Endophytic fungi can co-evolve with host plants for a long time, promote plant growth, and improve plant stress resistance. As potential biocontrol factors, endophytic fungi can also achieve the purpose of disease prevention and control by competing with pathogenic bacteria for nutrients and space. In recent years, the use of endophytic fungi as a biological preservative to prevent postharvest diseases has made significant progress. For example, Trichoderma sp. The inhibition rate of the mold fungus Penicillium italicum was over 70%. The crude extract...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00A01P1/00A23B7/154C12R1/77
CPCC12N1/14A23B7/154A23V2002/00C12R2001/77C12N1/145A23V2200/10A23V2250/208
Inventor 顾沛雯任苗苗闫思远张庆宸胡丽杰徐全智
Owner NINGXIA UNIVERSITY
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