Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Co-extraction method of betacyanin and plant salt in suaeda salsa

A technology of betaine and Suaeda salsa, applied in chemical instruments and methods, food science, organic dyes, etc., can solve the problems of increasing production costs, increasing the steps of removing heavy metals, cumbersome procedures, etc., and achieve alcohol elution The effect of high efficiency, short soaking and extraction time, and simplified preparation process

Active Publication Date: 2020-08-04
山东省蚕业研究所
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, red beet, dragon fruit, and Suaeda salsa are mostly used as the main raw materials for the extraction of betalain. The antioxidant activity of betalain is much weaker, so the same extraction and preparation technology has no reference for different raw materials
Although the extraction methods of betaine and plant salt in Suaeda salsa have also been reported in the literature, they are more common in the single extraction method of plant salt or betaine
[0004] Chinese patent document CN105124521A (application number 201510459908.3) discloses a method for extracting health-care plant salt from sea asparagus and Suaeda salsa. A method for extracting plant salt from Suaeda salsa by hot leaching process, but there is no extraction method involving betalain at the same time
Chinese patent document CN 102653634 A (application number 201110049205.5) discloses a method for extracting betalain from Suaeda salsa by ultrasonic assistance, and Chinese patent document CN 107841157A (application number 201711032939.6) discloses a kind of Suaeda salsa beet Red pigment extraction process, there is no extraction method involving plant salt
The preparation method has the problems of cumbersome procedures, many processing aids, and some additives have certain toxicity.
And there is no salt washing process in the extract after being absorbed by macroporous resin and chromatographic column, so there will be salt residue in the obtained betaine
[0006] At the same time, there is also a problem of heavy metal residues in the above-mentioned technology
Because Suaeda salsa grows in saline-alkali soil and other soils, the content of heavy metals generally exceeds the standard. In the prior art, in order to make the final product meet the relevant standards, it is necessary to increase the steps of removing heavy metals, which greatly increases the production cost.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Co-extraction method of betacyanin and plant salt in suaeda salsa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for co-extraction of betarubin and plant salt in Suaeda salsa, comprising the following steps:

[0040] (1) Place the fresh Suaeda salsa at -18°C for 48 hours, then place it in a vacuum freeze dryer for drying. Drying conditions: vacuum degree 25Pa, condensation temperature -45°C, take it out and crush it through an 80-mesh sieve. It is extracted with pure water at a weight ratio of 1:8 at room temperature for 20 minutes at a time, and is squeezed and filtered to obtain an extract and a residue. Mixing the primary extraction residue with purified water in a weight ratio of 1:1.5, and then leaching for 20 minutes at room temperature, then squeezing to remove the filter residue to obtain a secondary extraction solution, mixing the primary extraction solution and the secondary extraction solution to obtain a mixed extraction solution;

[0041] (2) Centrifuge the mixed extract obtained in step (1) at 5000r / min for 10min, remove the bottom layer and take the supernatant,...

Embodiment 2

[0048] A method for co-extraction of betaine and plant salt in Suaeda salsa, including the following steps:

[0049] (1) Place the fresh Suaeda salsa at -12°C for 55h and then place it in a vacuum freeze dryer to dry. Drying conditions: vacuum degree 35Pa, condensation temperature -35°C, take it out and crush it through a 100-mesh sieve. It is extracted with pure water at 1:12 room temperature for 40 minutes at a time, and then squeezed and filtered to obtain an extract and a residue. Mixing the primary extraction residue with purified water in a weight ratio of 1:2.5, and then leaching for 40 minutes at room temperature, then squeezing and removing the filter residue to obtain a secondary extraction solution, mixing the primary extraction solution and the secondary extraction solution to prepare a mixed extraction solution;

[0050] (2) Centrifuge the mixed extract obtained in step (1) at 5000r / min for 15min, remove the bottom layer precipitation and take the supernatant, add corn...

Embodiment 3

[0057] The co-extraction method as described in Example 1, the difference is that it does not include "add potato starch at a ratio of 0.5g / L and stir evenly, store at 4℃ for 72h, stir once every 12h, refrigerate After centrifugation at 5000r / min for 10 minutes, remove the bottom sediment and take the supernatant.

[0058] Test results

[0059] Table 1

[0060] Test items Example 1 Example 2 Example 3 Detection method Beetatin extraction yield / g / g4.544.464.01 Weighing method Plant salt extraction yield / g / g25.624.924.6 Weighing method Renewable times / time of macroporous resin1086 / Betarubin purity / g / 100g99.8298.5496.57 High performance liquid chromatography

[0061] It can be seen from Table 1 that comparing Example 1 with Example 2, the pre-freezing conditions and the changes in the types of adsorbed starch under the conditions of Example 1 are all higher than those of Example 2, indicating that different conditions have Betarubin and plant salt yield and the adsorption effec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a co-extraction method of betacyanin and plant salt in suaeda salsa, which comprises the following steps: (1) freezing the suaeda salsa, carrying out vacuum freeze drying, andpulverizing to obtain freeze-dried powder; (2) mixing the freeze-dried powder with water, carrying out primary extraction and secondary extraction, combining the primary extracting solution and the secondary extracting solution, carrying out solid-liquid separation, and taking the liquid to obtain an extracting solution; (3) adsorbing the extracting solution with macroporous resin, then washing with water to remove salt, and collecting a washing solution to obtain a salt solution; then eluting with alcohol, and collecting eluent to prepare a betacyanin solution; (4) concentrating and drying the salt solution, and removing organic impurities to obtain suaeda salsa plant salt; and (5) concentrating and drying the betacyanin solution to obtain betacyanin. According to the method, the fresh suaeda salsa is treated through pre-freezing for the first time, cell disruption can be conducted, separation of heavy metal ions and extracts is achieved, and the subsequent heavy metal removal step is completely omitted.

Description

Technical field [0001] The invention relates to a co-extraction method of betarubin and plant salt in Suaeda salsa, belonging to the technical field of chemical extraction. Background technique [0002] Suaeda salsa, also known as Suaeda salsa, is a typical saline-alkali indicator plant. It is widely distributed in my country's northeast, Inner Mongolia, Hebei, Shandong, Jiangsu and other coastal provinces. Suaeda salsa grows in coastal intertidal zones and depressions throughout the entire During the growing season, the above-ground parts are purple-red. The plant contains a large amount of sodium chloride, potassium chloride, calcium, magnesium ions and beta red pigment. It is an ideal raw material for extracting plant salt and beta red pigment. [0003] Betarubin is a water-soluble natural plant pigment with strong antioxidant biological activity, anti-cancer, anti-virus, and hypolipidemic functions. It is widely used in food, medicine, cosmetics and other fields. Plant salt is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23L27/40A23L5/20C09B61/00C09B67/54
CPCA23L27/40A23L5/20A23L5/273C09B61/00C09B67/0096A23V2002/00A23V2250/1582A23V2250/1614A23V2250/16A23V2250/161A23V2250/1578A23V2250/21A23V2250/5118A23V2300/14A23V2300/20
Inventor 范作卿施新琴郭洪恩王向誉王娜朱琳
Owner 山东省蚕业研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com