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Plant fermentation product and application thereof to preparation of composition for protecting lung and resisting oxidation

A plant fermentation and composition technology, applied in the field of fermentation, can solve the problems of expensive products, harmful to human health, etc.

Active Publication Date: 2020-08-04
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, most of the lung-protecting medicines currently used are made of chemical ingredients. Long-term use is not only harmful to human health, but also these products are often expensive and cannot be afforded by ordinary users

Method used

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  • Plant fermentation product and application thereof to preparation of composition for protecting lung and resisting oxidation
  • Plant fermentation product and application thereof to preparation of composition for protecting lung and resisting oxidation
  • Plant fermentation product and application thereof to preparation of composition for protecting lung and resisting oxidation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1. Preparation of plant fermented product

[0038] First, mix Imperatae Rhizoma, Polygonatum odoratum and Sterculia lychnophora from China with water at a volume ratio of 1 to 5:1:40 to 60, and heat at 50°C to 100°C Sterilize and extract for 0.5 to 3 hours respectively to obtain a plant extract. The plant extract was cooled to room temperature for subsequent three-stage fermentation. The three-stage fermentation is to inoculate the plant extract with 0.01-0.5% (v / v) Saccharomyces cerevisiae BCRC 20271 and 0.01-0.25% (v / v) Lactobacillus plantarum TCI028 (BCRC 910805 ) after 1 to 5 days of fermentation; inoculate 1 to 20% (v / v) of acetic acid bacteria (Acetobacter aceti) BCRC 11688 (the above strains were all purchased from the Food Industry Research and Development Institute (FIRDI) in Taiwan, China ) in the Biosource Collection and Research Center (BCRC)) for 3 to 8 days of fermentation. Thereafter, it was concentrated under reduced pressure at 45°C to 70°...

Embodiment 2

[0039] Example 2. Detection of total polyphenols and total flavonoids content of plant fermented product

[0040] First, to prepare a standard solution, 10 g of gallic acid was dissolved in water and a volume of 10 mL was added to a measuring bottle. Next, standard solutions of 0 μL / mL, 20 μL / mL, 40 μL / mL, 60 μL / mL, 80 μL / mL, and 100 μL / mL were prepared respectively, and then 100 μL of each standard solution was taken to a centrifuge tube having a volume of 10 mL. After that, 500 μL of Folin-Ciocalteu's phenol reagent was added, mixed and stood upright for 3 minutes, followed by 400 μL of 7.5% sodium carbonate, mixed and stood upright for 30 minutes. Next, 200 μL of each reaction solution was transferred to a 96-well plate, and the absorbance was measured at 750 nm.

[0041] In addition, the plant fermented product obtained in Example 1 was used as an experimental group, and the plant extract was used as a comparison group. Dilute the experimental group and the comparison gr...

Embodiment 3

[0047] Example 3. Efficacy Evaluation of Plant Fermented Products in Protecting the Lungs

[0048] First, human monocytic cell (human monocytic cell) THP-1 (corresponding to ATCC, TIB202) was cultured in RPMI-1640 medium (Gibco) (added with 10% fetal bovine serum (fetal bovine serum, FBS) (Gibco ), 0.05mM 2-mercaptoethanol (2-mercaptoethanol), 100units / mL penicillin (penicillin) and 100μg / mL streptomycin (streptomycin)). Add medium to each well of the 6-well culture plate (GeneDireX), so that each well has 5 x 10 5 THP-1 cells. Then, add differentiation medium with phorbol-12-myristate13-acetate (phorbol 12-myristate13-acetate, PMA, Sigma) 2 cultured under the culture conditions for 48 hours. Afterwards, fresh medium was replaced and cultured for another 48 hours.

[0049] In addition, a single plant fermented product (comprising a single fermented product of Imperata coronata, a single fermented product of Polygonatum odoratum and a single fermented product of Pterodactac...

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Abstract

The invention relates to the field of fermentation, in particular to a plant fermentation product and application of the plant fermentation product to preparation of a composition for protecting lungand resisting oxidation. The invention provides the plant fermentation product, which is prepared by a method comprising the following steps of: (a) extracting a combination consisting of lalang grassrhizome, fragrant solomonseal rhizome and boat-fruited sterculia seed by using water to obtain a plant extract; (b) sequentially fermenting the plant extract, saccharomyces cerevisiae, germ lactic acid bacteria and acetic acid bacteria to obtain a plant fermentation product. The invention also provides application of the plant fermentation product in lung protection and oxidation resistance.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a plant fermented product and its use for preparing a lung-protecting and anti-oxidation composition. Background technique [0002] Lung injury, mainly refers to damage to lung tissue, including: severe chest trauma, inhalation of substances harmful to the lungs (such as toxic gases, stomach contents, and seawater), severe lung infection and other injury factors caused by lung tissue Injuries that result in disruption of the structural integrity or dysfunction of the lungs. [0003] In addition, air pollution has become more and more serious in recent years. When the sky is foggy, it is often when there is a lot of dust in the air. Dust can be divided into different levels such as large particles and extremely fine particles. When dust is inhaled, there will be two As a result, large ones can be carried out of the body with expectoration. PM2.5 is a kind of allergen, but this allerg...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P39/06A61P11/00A23L33/105
CPCA61K36/899A61K36/8969A61K36/185A61P39/06A61P11/00A23L33/105A61K2236/19A61K2300/00
Inventor 林咏翔庄伟秀
Owner TCI CO LTD
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