The invention relates to high-nutrition glutinous rice flour and a preparation method thereof. The high-nutrition glutinous rice flour is characterized by being prepared from the following raw materials in parts by weight: 40-45 parts of glutinous rice, 20-25 parts of black rice, 10-13 parts of pearl barley, 3-4 parts of leaf mustard, 5-7 parts of kalimeris indica, 8-10 parts of asparagus lettuce, 2-3 parts of tabernaemontana divaricata, 1-2 parts of common frangipani, 3-4 parts of sevenlobed yam rhizome, 2-4 parts of Chinese gentian root, 2.5-4 parts of barley grass, 1-2 parts of seabuckthorn leaf, 2-3 parts of red claw ginger, 1-2 parts of largehead atractylodes rhizome, 2-3 parts of thunberg fritillary bulb, 4-5 parts of licorice root and 0.1-0.2 part of amylase. The high-nutrition glutinous rice flour provided by the invention is soft, tough and smooth, peanuts, hazelnuts and the like are added during the processing process, and the nutrition is rich; furthermore, the high-nutrition glutinous rice flour is fragrant and delicious and can simultaneously achieve an anti-oxidation effect after being stir-fried with grape seed oil; and in addition, a variety of Chinese herbal medicines are added into the high-nutrition glutinous rice flour, so that the high-nutrition glutinous rice flour has the effects of clearing heat, moistening lung, relaxing bowels, nourishing kidneys, harmonizing qi, detoxicating and improving eyesight.