Check patentability & draft patents in minutes with Patsnap Eureka AI!

Application of flammulina velutipes polysaccharide extract in preparation of antiviral drugs and extraction method of flammulina velutipes polysaccharide extract

A technology of Flammulina velutipes polysaccharide and antiviral drug, which can be used in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of antigenic drift, antigenic transformation, influenza hysteresis, etc., and relieve respiratory symptoms. , The effect of inhibiting cytopathic and low production cost

Active Publication Date: 2020-08-25
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Vaccination is the most effective way to prevent and control the spread of influenza, but the main antigens of influenza virus, hemagglutinin (HA) and neuraminidase (neuraminidase, NA) are prone to antigenic shift and antigenic drift, leading to human Emerging strains lack immunity, causing influenza virus outbreaks
Therefore, there is a certain lag in preventing influenza through vaccination, and the preventive effect is limited
The drugs used to treat influenza viruses are mainly amantadines (such as amantadine and rimantadine) and neuraminidase inhibitors (such as oseltamivir and zanamivir), but these drugs are due to The emergence of drug resistance and its inherent toxic side effects limit clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of flammulina velutipes polysaccharide extract in preparation of antiviral drugs and extraction method of flammulina velutipes polysaccharide extract
  • Application of flammulina velutipes polysaccharide extract in preparation of antiviral drugs and extraction method of flammulina velutipes polysaccharide extract
  • Application of flammulina velutipes polysaccharide extract in preparation of antiviral drugs and extraction method of flammulina velutipes polysaccharide extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1 Flammulina velutipes polysaccharide extract

[0045](1) Take the fruiting bodies of Flammulina velutipes, pulverize them with a pulverizer (25000r / min, 5s), use the No. 1 Pharmacopoeia inspection sieve to screen, collect the filtrate, add it to 15 times the weight of water to soak until it is completely wet, and heat and extract for 1.5h , filter, and collect the filtrate; re-add water to the filter residue 10 times, heat and extract for 1 hour, filter, collect the filtrate, combine the obtained filtrate, concentrate under reduced pressure at 50°C to about 20L, and further concentrate to about 60% of the barley degree to obtain a concentrated solution;

[0046] (2) Add dehydrated ethanol to the concentrated solution obtained in step (1) so that the volume fraction of dehydrated alcohol is 85%, let it stand overnight, take the precipitate and transfer it to a baking tray, and dry it in vacuum at 45°C to obtain the extract of Flammulina velu...

Embodiment 2

[0047] Cytotoxicity research of embodiment 2 Flammulina velutipes polysaccharide

[0048] The polysaccharide extract of Flammulina velutipes obtained in Example 1 was diluted with PBS to prepare a 3 mg / mL drug stock solution, and stored at 4°C.

[0049] MDCK cells (canine kidney cells) in good growth state were digested with EDTA-trypsin and counted at 2×10 4 / 100μL / well seeded cells in 96-well plate, 37°C, 5% CO 2 Cell culture incubator for 24 hours. When the cell confluence is nearly 90%, the cell culture medium is removed, and the cells are washed twice with 1×PBS (phosphate buffered saline). The drug mother solution was serially diluted by 2 times in DMEM containing 2% fetal bovine serum, and the concentrations of the drug dilutions were 100 μg / mL, 200 μg / mL, 400 μg / mL, 800 μg / mL, 1000 μg / mL, and 2000 μg / mL. Add the drug solution to the above-mentioned cell culture plate at 100 μL / well, add 100 μL / well of the blank control well and the normal cell control well to the ME...

Embodiment 3

[0054] Example 3 Anti-influenza Virus Research of Flammulina velutipes Polysaccharides in Vitro

[0055] MDCK cells in good growth state were divided into about 2 × 10 per well 4 Density seeded into 96-well cell culture plates at 37°C, 5% CO 2 The cells were cultured in the incubator for about 24 hours, and the degree of cell confluence was nearly 100%. The cell culture medium was removed, the cells were washed twice with 1×PBS, and different drug stock solutions were serially diluted by 2 times with DMEM containing 2% fetal bovine serum. The Flammulina velutipes polysaccharide extract obtained in Example 1 was diluted with PBS to make a medicinal solution and added to the above-mentioned cell culture plate at 50 μL / well, and 50 μL / well of virus control wells and normal cell control wells were added with 2% fetal bovine serum. DMEM basal medium, 37°C, 5% CO 2 Continue culturing in the cell culture incubator. At the same time, 25 μL of the drug solution was mixed with 25 μL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a medical application of a polysaccharide extract, in particular to an application of a flammulina velutipes polysaccharide extract in preparation of anti-influenza virus drugs and an extraction method of the flammulina velutipes polysaccharide extract. The invention provides the application of the flammulina velutipes polysaccharide extract in preparation of antiviral drugs, foods or health products. The invention provides that the flammulina velutipes polysaccharide extract has a clear anti-influenza virus effect for the first time, in-vitro anti-influenza virus efficacy research is carried out on the flammulina velutipes polysaccharide extract obtained through extraction, and results show that the flammulina velutipes polysaccharide extract can significantly inhibit cytopathy caused by influenza virus infection. Animal experiments (H1N1 virus infected mouse experiments) are carried out on the flammulina velutipes polysaccharide extract obtained through extraction, and the result proves that the flammulina velutipes polysaccharide extract can effectively relieve respiratory tract symptoms caused by H1N1 virus infection. It is indicated that the flammulinavelutipes polysaccharide extract has good prospects when applied to preparation of the anti-influenza virus medicine.

Description

technical field [0001] The present invention relates to the medical application of polysaccharide extract, in particular, relates to the application of the polysaccharide extract of Flammulina velutipes in the preparation of anti-influenza virus medicine and its extraction method. Background technique [0002] Influenza is an acute respiratory disease caused by influenza virus (Influenza Virus), which has the characteristics of strong infectivity, rapid spread, and widespread prevalence, which seriously threatens human life and health and poses a huge economic threat. Human influenza virus is a kind of influenza virus, which is divided into three types: A (A), B (B), and C (C). Among them, type A influenza virus is the most threatening to humans because of its antigenicity that is most likely to mutate. The 2009 influenza A (H1N1) pandemic occurred worldwide, highlighting the severity of influenza A virus infection. [0003] Vaccination is the most effective way to prevent ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P31/18A61K36/07
CPCA61K31/715A61K36/07A61P31/18
Inventor 苏冀彦吕淑媚谢意珍吴清平
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com