Preparation method and application of chemotherapy-phototherapy synergetic anti-tumor microsphere

An anti-tumor and microsphere technology, used in anti-tumor drugs, photodynamic therapy, medical preparations with inactive ingredients, etc., can solve the problems of low feasibility, excessive use, difficult industrial production, etc., and achieve strong compliance , enhance drug resistance, and have the effect of industrial production feasibility

Inactive Publication Date: 2020-08-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to achieve the effect of multi-strategy combination therapy, many studies have designed overly complex drug carriers, which are difficult to industrialize, and even excessively use ingredients that have not been approved by the State Drug Administration, and the feasibility of practical application is low.

Method used

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  • Preparation method and application of chemotherapy-phototherapy synergetic anti-tumor microsphere
  • Preparation method and application of chemotherapy-phototherapy synergetic anti-tumor microsphere
  • Preparation method and application of chemotherapy-phototherapy synergetic anti-tumor microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take two 50mL dry screw-top bottles and add the prepared 2% sodium alginate solution, and then add 40 μg / mL chemotherapeutic drug doxorubicin (DOX) and 10% cellulose nanocrystals (CNC) to one of the bottles, Add 2.5 μg / mL photosensitizer indocyanine green (ICG) to the other bottle, and mix the two bottles of solutions evenly, as the internal and external phases of chemotherapy-phototherapy synergistic anti-tumor core-shell microspheres. Take another receiving dish without a cover, add an aqueous solution containing 2% calcium chloride and 0.15% chitosan, and the solution depth exceeds 1 cm, as a receiving bath for the microspheres. Using gas-assisted method, add sodium alginate solution containing DOX and CNC to the internal phase channel, add sodium alginate solution containing ICG to the external phase channel, and then pass nitrogen gas into the outermost layer at a flow rate of 6L / min to form core-shell droplets, and solidified by a receiving bath to finally form an...

Embodiment 2

[0033] Both the free ICG and the microspheres were placed in a cuvette, and the full-wavelength absorbance was measured with a UV-visible spectrophotometer, as shown in Figure 4 As shown in (A), both the free ICG and the ICG encapsulated in microspheres have the maximum absorption peak at 780nm.

[0034] Both free ICG and microspheres were incubated in PBS at 37°C for 5 days. The absorbance at 780 nm was measured with a UV-vis spectrophotometer every day, such as Figure 4 As shown in (B), the free ICG was obviously degraded, but its stability was significantly improved after being encapsulated by microspheres.

[0035] Both free ICG and microspheres were placed in 25 μg / mL DPBF solution, and the DPBF solution without any other substances was used as a blank control. Use 0.8W / cm 2 808nm near-infrared laser irradiation for 5 minutes, and measure the absorbance at 410nm with a UV-visible spectrophotometer every minute, as Figure 4 (C) shown. Both the free ICG and the ICG ...

Embodiment 3

[0039] HepG-2 cells were placed in DMEM medium containing 10% fetal bovine serum and cultured at 37°C in a cell culture incubator with 95% sterile air and 5% carbon dioxide environment. Change the cell culture medium every two days.

[0040] 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) was used as a fluorescent probe to measure the level of intracellular ROS. HepG-2 cells were seeded in 12-well plates at a cell density of 1×10 5 One per well, cultured for 24 hours, then replaced the previous medium with free ICG or microspheres and cultured for 8 hours, and then added 10 μg / mL DCFH-DA. After incubating for 20 minutes, remove the medium, wash the cells three times with PBS buffer, and use 808nm laser (0.8W / cm2 ) for 3 minutes, observed cell morphology, and imaged with a fluorescent microscope (IX 53, OLYMPUS, Japan), such as Figure 7 shown.

[0041] The killing effect of chemotherapy-phototherapy combined with anti-tumor core-shell microspheres on cancer cell HepG-2...

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Abstract

The invention discloses a chemotherapy-phototherapy synergetic anti-tumor microsphere. The microsphere achieves the synergetic anti-tumor effect that 1 plus 1 is greater than 2 simultaneously throughthe combined action of chemotherapy, photodynamic therapy and photothermal therapy, and solves the problem of great toxic and side effects of traditional chemotherapeutic drugs. The invention furtherdiscloses a preparation method and application of the anti-tumor microsphere. The preparation method disclosed by the invention is green and simple, used auxiliary materials and medicines are components which are approved by China and can be used as medicines, introduction of unnecessary components is avoided, and the microsphere has higher safety and compliance.

Description

technical field [0001] The invention belongs to the technical field of biomaterial application, and in particular relates to chemotherapy-phototherapy synergistic anti-tumor microspheres. Background technique [0002] Cancer is a major disease that seriously endangers human health, and its mortality rate accounts for the second place in the overall mortality rate of human diseases. Chemotherapy is an important means of cancer treatment. However, traditional chemotherapy drugs can easily cause toxic and side effects such as liver and kidney damage, bone marrow suppression, and reduced human immunity. Therefore, suitable attenuated chemotherapy drug carriers are very important. On the other hand, a large number of studies have shown that synergistic therapeutic approaches have important efficacy in enhancing drug resistance and tumor recurrence. Photodynamic therapy (PDT) and photothermal therapy (PTT) are currently important research areas in tumor therapy, and they usually ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/50A61K31/704A61K45/06A61K47/36A61K47/38A61P35/00
CPCA61K41/0057A61K41/0052A61K31/704A61K45/06A61K9/5036A61K9/5047A61P35/00
Inventor 黄超伯曲清莉张建陈小琼唐国胜
Owner NANJING FORESTRY UNIV
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