Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of fluorescent gold nanocluster, prepared fluorescent gold nanocluster and application of fluorescent gold nanocluster

A fluorescent gold nanometer and fluorescent probe technology, applied in the preparation of fluorescent gold nanoclusters, the field of fluorescent gold nanoclusters, can solve the problems of complex synthesis steps of molecular fluorescent probes, poor anti-photobleaching performance, poor analytical selectivity, etc. The effect of uniform size, enhanced solubility and dispersibility, strong fluorescence stability

Active Publication Date: 2020-08-28
ANHUI MEDICAL UNIV
View PDF11 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the determination of hROS tends to have higher sensitivity and is more suitable for fluorescence analysis. However, molecular fluorescent probes have complex synthesis steps, poor photobleaching resistance, high biological toxicity and sensitivity to spontaneous autoxidation. Other ROS Or the presence of RNS will interfere with the detection of hROS, leading to disadvantages such as poor analytical selectivity, which limits its further application in the field of bioanalysis and bioimaging research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of fluorescent gold nanocluster, prepared fluorescent gold nanocluster and application of fluorescent gold nanocluster
  • Preparation method of fluorescent gold nanocluster, prepared fluorescent gold nanocluster and application of fluorescent gold nanocluster
  • Preparation method of fluorescent gold nanocluster, prepared fluorescent gold nanocluster and application of fluorescent gold nanocluster

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Preparation of fluorescent gold nanocluster SLD-AuNCs:

[0101] (1) Pre-gelatinization of soluble starch: put 0.25g of soluble starch in a round bottom flask, add 20mL of double distilled water to suspend, stir and heat on an oil bath until boiling, and heat the soluble starch until it is transparent and without obvious granular , continue to heat and boil for 20 minutes, then cool to room temperature to prepare 1.25% pre-gelatinized starch and set aside;

[0102] (2) Use aqua regia (nitric acid / hydrochloric acid, the volume ratio is 1:3) to thoroughly clean the glassware used, and clean it with double distilled water;

[0103] (3) prepare the chloroauric acid aqueous solution containing 1.25% pregelatinized starch (the final concentration of chloroauric acid aqueous solution is 8.6 × 10 -4 M), heated to vigorous boiling under stirring, take 7mL concentration to be 8.6×10 -4 M's chloroauric acid aqueous solution was added to 1mL with a concentration of 3.45×10 -3 M L...

Embodiment 2

[0106] The difference between this example and Example 1 is that the aqueous solution of levodopa is replaced by an aqueous solution of dopamine, and the concentration of the aqueous solution of dopamine in this example is 0.6 mM.

Embodiment 3

[0108] The difference between this example and Example 1 is that the levodopa aqueous solution is replaced by the protocatechualdehyde aqueous solution, and the concentration of the protocatechualdehyde aqueous solution in this example is 1 mM.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a fluorescent gold nanocluster, and relates to the technical field of material preparation. The preparation method comprises the following steps that polysaccharide is used as a template, chloroauric acid reacts with a polyphenol solution to prepare the fluorescent gold nanocluster. The invention further discloses the fluorescent gold nanocluster prepared through the preparation method, a preparation method of the catechol-borate compound fluorescent gold nanocluster, the prepared catechol-borate compound fluorescent gold nanocluster and application of the catechol-borate compound fluorescent gold nanocluster serving as a fluorescent probe. The preparation method has the beneficial effects that the polysaccharide is used as the template, polyphenol is used as a reducing agent, and the fluorescent gold nanocluster is prepared, so that the solubility and the dispersibility of the obtained fluorescent gold nanocluster can be enhanced; boracis acis is used as a protective agent, the prepared catechol-borate compound fluorescent gold nanocluster has good biocompatibility, physiological required conditions are well met, and the catechol-borate compound fluorescent gold nanocluster has high selectivity in detection of intracellular high-activity oxygen level changes.

Description

technical field [0001] The invention relates to the technical field of material preparation, in particular to a method for preparing fluorescent gold nanoclusters, the prepared fluorescent gold nanoclusters and applications thereof. Background technique [0002] With the rapid development of nanotechnology, nano-fluorescent materials, as emerging fluorescent materials, have attracted the attention of more and more scientific researchers, and also provide a good development opportunity for the design and development of nano-biosensors. At present, the widely studied fluorescent nanomaterials mainly include carbon dots, graphene quantum dots, semiconductor quantum dots, and fluorescent metal nanoclusters. As a new fluorescent material, fluorescent metal nanoclusters have the advantages of unique optical properties, low biological toxicity, good light irradiation stability and biocompatibility, large Stokes shift and convenient preparation methods, etc. These excellent propert...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B22F9/24B22F1/00C09K11/58G01N21/64B82Y40/00
CPCB22F9/24C09K11/58G01N21/64G01N21/6402G01N21/6428G01N21/6458B82Y40/00G01N2021/6421G01N2021/6432G01N2021/6495G01N2021/6497B22F1/054
Inventor 张群林方胡凤余欢卢麒方栒
Owner ANHUI MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com