A kind of bacillus licheniformis and its screening method and application

A technology for Bacillus licheniformis and Bacillus licheniformis, which is applied in the field of microorganisms and achieves the effects of high application value, high production safety and reduced production costs

Active Publication Date: 2022-04-12
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report of using bacillus licheniformis to prepare bio-organic fertilizer by using distiller's grains

Method used

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  • A kind of bacillus licheniformis and its screening method and application
  • A kind of bacillus licheniformis and its screening method and application
  • A kind of bacillus licheniformis and its screening method and application

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Experimental program
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Effect test

Embodiment 1

[0033] A kind of Bacillus licheniformis in the present invention, classified and named as Bacillus licheniformis JZLGZ-1, the preservation number is CGMCC No.18747, the preservation date is October 28, 2019, and the preservation unit is China Microorganism Culture Preservation Management Committee Ordinary Microbiology Center, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

Embodiment 2

[0035] The nucleotide sequence of Bacillus licheniformis is shown as SEQ ID No: 1, and the screening steps are as follows:

[0036] (1) Take samples in the brewing workshop of the liquor factory, select the discarded liquor after distillation and throw it away, and put it into a sterile sampling bag.

[0037] (2) Weigh 10g of distilled spirits and place in a 100mL Erlenmeyer flask, add 90mL of sterile saline, and shake well;

[0038] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10-3 、10 -4 times the diluted solution;

[0039] (4) Use a sterile pipette gun to pipette 100 μL of each dilution and apply it to 30 mL of Congo red culture medium, and spread the bacterial solution evenly on the surface of the medium with a coating stick; The surface of the rear medium is not easy to dry, and it is easy to cause bacterial contamination when inverted;

[0040] (5) Place in a constant temperature incubat...

Embodiment 3

[0047] The screening steps of Bacillus licheniformis strains are as follows:

[0048] (1) Take samples in the brewing workshop of the liquor factory, select the discarded liquor after distillation and throw it away, and put it into a sterile sampling bag.

[0049] (2) Weigh 10g of distilled spirits and place in a 100mL Erlenmeyer flask, add 90mL of sterile saline, and shake well;

[0050] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10 -3 、10 -4 times the diluted solution;

[0051] (4) Use a sterile pipette gun to pipette 100 μL of each dilution solution into the Congo red medium, and spread the bacterial solution evenly on the surface of the medium with a coating stick;

[0052] (5) placed in a constant temperature incubator at 37°C for 2 days;

[0053] (6) Select the bacterium that is a single colony in the above-mentioned medium, and pick the bacterium that produces the hydrolysis circle r...

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Abstract

The invention relates to the field of microorganisms, in particular to a bacillus licheniformis and a screening method and application thereof. The Bacillus licheniformis strain used in the present invention was screened from liquor distilled grains. The preservation number of the strain is CGMCC No.18747, the preservation date is October 28, 2019, and the preservation unit is the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The strain is easy to cultivate and has high enzyme activity. The organic matter (dry basis) content of the bio-organic fertilizer prepared by fermenting liquor waste grains with this strain exceeds 90%, and the effective number of viable bacteria (CFU) reaches 0.7-110 million / g; after fermentation, the pH is 5.5-6.5, containing The water content is 15% to 20%, all within the national standard range (the national standard requires organic matter (dry basis) content ≧ 40.0%, effective viable count (CFU) ≧ 0.2 billion / g, moisture ≦ 30.0%, pH 5.5 ~ 8.5), with high application value.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a bacillus licheniformis and its screening method and its application in preparing bio-organic fertilizer by using distilled grains. Background technique [0002] Waste grains are one of the most important by-products of liquor production. With the increase of liquor production, the output of waste grains is also increasing. The production of 1,000 liters of liquor will produce about 8 tons of waste grains. If it is not handled properly or discarded directly, it will inevitably cause environmental pollution. It is also used to directly make compost and feed, but the utilization rate is not high, resulting in waste of resources. How to make good use of waste has become an urgent problem to be solved in the liquor industry. Waste grains are rich in nutrients, mainly containing cellulose, protein and starch, among which cellulose accounts for 17-21%, and the cellulose content is very high....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/34C12Q1/04C05F17/20C12R1/10
CPCC12N1/20C12N1/02C12Q1/34C12Q1/04C12R2001/10C12N1/205Y02W30/40
Inventor 赵长青蒋佳林高子晴罗路南罗惠波赵阳
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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