A kind of bacillus licheniformis and its screening method and application
A technology for Bacillus licheniformis and Bacillus licheniformis, which is applied in the field of microorganisms and achieves the effects of high application value, high production safety and reduced production costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] A kind of Bacillus licheniformis in the present invention, classified and named as Bacillus licheniformis JZLGZ-1, the preservation number is CGMCC No.18747, the preservation date is October 28, 2019, and the preservation unit is China Microorganism Culture Preservation Management Committee Ordinary Microbiology Center, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
Embodiment 2
[0035] The nucleotide sequence of Bacillus licheniformis is shown as SEQ ID No: 1, and the screening steps are as follows:
[0036] (1) Take samples in the brewing workshop of the liquor factory, select the discarded liquor after distillation and throw it away, and put it into a sterile sampling bag.
[0037] (2) Weigh 10g of distilled spirits and place in a 100mL Erlenmeyer flask, add 90mL of sterile saline, and shake well;
[0038] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10-3 、10 -4 times the diluted solution;
[0039] (4) Use a sterile pipette gun to pipette 100 μL of each dilution and apply it to 30 mL of Congo red culture medium, and spread the bacterial solution evenly on the surface of the medium with a coating stick; The surface of the rear medium is not easy to dry, and it is easy to cause bacterial contamination when inverted;
[0040] (5) Place in a constant temperature incubat...
Embodiment 3
[0047] The screening steps of Bacillus licheniformis strains are as follows:
[0048] (1) Take samples in the brewing workshop of the liquor factory, select the discarded liquor after distillation and throw it away, and put it into a sterile sampling bag.
[0049] (2) Weigh 10g of distilled spirits and place in a 100mL Erlenmeyer flask, add 90mL of sterile saline, and shake well;
[0050] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10 -3 、10 -4 times the diluted solution;
[0051] (4) Use a sterile pipette gun to pipette 100 μL of each dilution solution into the Congo red medium, and spread the bacterial solution evenly on the surface of the medium with a coating stick;
[0052] (5) placed in a constant temperature incubator at 37°C for 2 days;
[0053] (6) Select the bacterium that is a single colony in the above-mentioned medium, and pick the bacterium that produces the hydrolysis circle r...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com