Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus plantarum and microecological preparation, preparation method and application thereof

A technology of Lactobacillus plantarum and microecological preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, Lactobacillus, etc., can solve the problems of unsatisfactory effect and stability of lactic acid bacteria, and achieve the prevention and treatment of Mycoplasma gallisepticum disease, improve the body's immunity, good safety effect

Active Publication Date: 2020-09-08
山东宝来利来生物工程股份有限公司
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that with the continuous in-depth research on probiotics with immune activity, the effect and stability of some lactic acid bacteria with probiotic functions are not satisfactory

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus plantarum and microecological preparation, preparation method and application thereof
  • Lactobacillus plantarum and microecological preparation, preparation method and application thereof
  • Lactobacillus plantarum and microecological preparation, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Initial screening of immunocompetent lactic acid bacteria

[0043] 1Materials and methods

[0044] 1.1 Material

[0045] MRS medium: calculated by mass percentage, glucose 2.0%, sodium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, Yeast extract 0.5%, Tween-80 0.1%, pH 6.0.

[0046] IEC-6 cells (small intestinal epithelial cells) were purchased from the cell bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.

[0047] 1.2 method

[0048] 1.2.1 Preparation of bacterial suspension

[0049] Twenty strains of lactic acid bacteria provided by the Culture Collection Center of Bioengineering Research Institute of Shandong Baolai Lilai Bioengineering Co., Ltd. were respectively inoculated into MRS liquid medium, cultured at 37°C for 16-18h, and centrifuged at 4°C 3000rpm for 15min to collect the bacteria. The cells were suspended in PBS solut...

Embodiment 2

[0075] Example 2 Rescreening of immunocompetent lactic acid bacteria

[0076] 1Materials and methods

[0077] 1.1 Material

[0078] MRS medium: calculated by mass percentage, glucose 2.0%, sodium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, Yeast extract 0.5%, Tween-80 0.1%, pH 6.0. The solid medium needs to add 1.5% agar powder.

[0079] Improved MRS medium: Use sucrose instead of glucose, and the others are the same as MRS medium.

[0080] 1.2 method

[0081] 1.2.1 Primary screening of extracellular polysaccharide-producing lactic acid bacteria (colony drawing method)

[0082] The 8 strains of lactic acid bacteria (see Table 4) initially screened in Example 1 were inoculated into the MRS medium for activation, and the activated lactic acid bacteria were inoculated into the modified MRS solid medium by the plate streaking method, and cultured at 37°C for 24 hours. Observe the...

Embodiment 3

[0097] Example 3 Lactic acid bacteria immune function test

[0098] 1Materials and methods

[0099] 1.1 Material

[0100] Healthy Kunming mice, purchased from Shandong Taibang Biological Products Co., Ltd.; Mouse Interleukin 2 (IL-2) ELISA Test Kit, Mouse Interferon-γ (IFN-γ) ELISA Kit, Mouse Immunoglobulin G (IgG) ELISA kits were purchased from Beijing Equation Biotechnology Co., Ltd.

[0101] 1.2 method

[0102] 1.2.1 Lactic acid bacteria immune function test

[0103] Choose 210 healthy Kunming mice, half male and half, weighing 18-20g, and rearing them in a quiet, warm, and avoiding bright environment for 3 days. After adapting for 3 days, they are randomly divided into 6 groups, each with 35 mice, which are the blank group and the model. Group, positive control group, No. 1 strain group, No. 15 strain group, No. 16 strain group. Before the start of the experiment, the other groups except the blank group were injected intraperitoneally with 80 mg / kg cyclophosphamide for 3 consecut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Absorbanceaaaaaaaaaa
Login to View More

Abstract

The invention provides lactobacillus plantarum and a microecological preparation, a preparation method and application thereof. The lactobacillus plantarum is named as Lactobacillus Plantarum BLCC2-0125, and is preserved in the China Center for Type Culture Collection in Wuhan City on April 24th, 2020, and the preservation number is CCTCC NO: M 2020078. The lactobacillus plantarum can improve thebody immunity, has a prevention effect on low-pathogenicity avian influenza, and can prevent and treat mycoplasma gallisepticum disease.

Description

technical field [0001] The invention relates to the field of immune microecology, in particular to a strain of Lactobacillus plantarum, its microecological preparation, its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Lactic acid bacteria widely exist in the natural environment and are essential beneficial bacteria in the intestinal tract of humans or animals. Lactic acid bacteria play an important role in the host's innate immune system and in maintaining the immune homeostasis of the body. It mainly plays a role by enhancing or restoring intestinal homeostasis, and has the potential to be a mucosal immune carrier. The adhesion of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23K10/18A23K10/12A23K50/75A61K35/747A61P31/16A61P31/04A61P37/04C12R1/25
CPCC12N1/20A23K10/18A23K10/12A23K50/75A61K35/747A61P31/16A61P31/04A61P37/04C12R2001/25C12N1/205A23V2400/169
Inventor 王春凤单宝龙宁扬宋翔侯玉凤李鹏陈晓雯刘乃芝亓秀晔郭杨丽程福亮陈雷谷巍徐海燕王红
Owner 山东宝来利来生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com