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Up-conversion bionic complex as well as preparation method and application in weak ultraviolet conversion

A complex and particle conversion technology, which is applied in the direction of luminescent/biological dyeing preparations, preparations for in vivo tests, drug combinations, etc., can solve problems such as adverse consequences, damage to irradiated parts, and traumatic unfavorable multiple times. Achieve the effect of increasing concentration and avoiding tissue damage

Active Publication Date: 2020-09-25
济南康硕生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the reported in vivo studies on the relationship between UV radiation and inflammation or AS all use the method of UV radiation on the body surface, and the effector cells of this method are actually macrophages in the skin, monocytes in the circulation and The systemic potential adverse effects brought about by T cells, etc. are also obvious: firstly, the inflammatory suppression effect brought about by irradiation may cause immunity decline and unexpected immune regulation disorders in other systems; secondly, macrophages in different tissues are extremely The biological effects of different cultures are different. Excessive induction of the M2 phenotype in tumor tissue may lead to tumor progression, so the effect of systemic UV irradiation on macrophage function may cause "off-target" and even lead to adverse consequences.
More importantly, it is difficult for a single UV irradiation to produce a sustained inflammatory regulatory effect on chronic inflammatory lesions, while repeated UV irradiation has the risk of inducing damage to the irradiated site and canceration due to dose accumulation
[0010] In recent years, extensive research has been carried out on the photobiology and molecular mechanisms caused by weak ultraviolet radiation. Due to the extremely poor penetration of ultraviolet biological tissues, it is difficult to reach deep tissues; It is possible, but it requires invasive operation, which is not only traumatic but also unfavorable for repeated operations. More importantly, due to the fast blood flow and the influence of blood cells, it is difficult for the ultraviolet rays transmitted by optical fibers to penetrate the entire vessel wall

Method used

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  • Up-conversion bionic complex as well as preparation method and application in weak ultraviolet conversion

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Embodiment 1

[0080] 1) Add β-NaYF 4 and YCl with EDTA 3 , YbCl 3 , TmCl 3 (Molar ratio 79:20:1) aqueous solution is fully mixed to form EDTA-rare earth element chelate emulsion, drop into NaF solution, heat at 200°C for 18 hours after rapid stirring, and rinse with deionized water and ethanol repeatedly after cooling to room temperature Precipitation, drying, annealing and crystallization at 400°C in a nitrogen atmosphere to obtain organic upconversion particles.

[0081] 2) The reference for the method of compounding upconversion particles with sensitizers and activators is Han J, etal. Amplification of Circularly Polarized Luminescence through Triplet–Triplet Annihilation-Based Photon Upconversion. Journal of the American Chemistry Society. 9783–9786. and Liu Q, et al. Highly Photostable Near-IR-Excitation Upconversion Nanocapsules Based on Triplet–Triplet Annihilation for in Vivo Bioimaging Application. ACS Applied Materials and Interfaces. 2018, 10(12):9883-9888. The sensitizer is ...

Embodiment 3

[0085] Construct and connect fluorescent probes for miR-155 detection: Based on the ssDNA sequence 5′-AAA CCC CTATCA CGA TTA-3′ for miR-155 detection, a double-stranded nucleotide chain was constructed, and the two ends of the two chains were connected to Cy5. 5 and BHQ-3 Black Hole Quencher. According to the FRET principle, when miR-155 binds to the ssDNA sequence, Cy5.5 is separated from BHQ-3 to release a fluorescent signal. The biodegradable dendritic polymer PAMAM is connected to the surface of the prepared core-shell type upconversion particles by carbodiimide chemical technology, and the miR-155 fluorescence detection probe is connected by using the positive charge adsorption of PAMAM.

Embodiment 4

[0087] Neutrophil membrane bionic packaging: collect peripheral blood from mice, collect neutrophils by Percoll gradient centrifugation, break up the cells, filter them with a filter with a pore size of 1 μm, and then centrifuge at 3200g. Centrifuge, collect the supernatant of the two centrifuges, filter and centrifuge at 20,000g for 20-30 minutes, then centrifuge the supernatant at 100,000g for 5 minutes, resuspend the pellet with 10mM Tris-HCl (pH7.5) and 1mM EDTA, Collect neutrophil membranes.

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PUM

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Abstract

The invention relates to an up-conversion bionic complex as well as a preparation method and application in weak ultraviolet conversion. A base material comprises core-shell type up-conversion particles and multiple effector molecules, the core-shell type up-conversion particles comprise a mesoporous silicon shell layer and up-conversion particles located inside the shell layer, and the effector molecules are modified on outer surfaces or in gaps of the core-shell type up-conversion particles; and the effector molecules have functions of macrophage phenotype transformation and inflammatory activity regulation. The up-conversion bionic complex is obtained by combining the base material with neutrophil membranes, and has good application effects in weak ultraviolet conversion and regulationof radiation.

Description

technical field [0001] The invention belongs to the technical field of weak ultraviolet conversion, and specifically relates to an up-conversion biomimetic complex, a preparation method and an application of weak ultraviolet conversion. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Atherosclerotic (AS) plaque rupture and thrombosis are the main pathological mechanisms leading to acute cardiovascular events. AS is a lipid-driven, chronic, systemic inflammatory lesion that occurs in the vessel wall. Inflammation runs through the entire process from the early stage of lesion, plaque formation to plaque instability and rupture. The pro-inflammatory and anti-inflammatory pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/59A61K47/62A61K47/69A61K41/00A61K9/51A61K47/46A61P9/10A61K49/00
CPCA61K47/59A61K47/595A61K47/62A61K47/6935A61K41/0004A61K9/5184A61P9/10A61K49/005A61K49/0093
Inventor 张鹏飞孙媛媛蒋妍彦
Owner 济南康硕生物技术有限公司