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Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof

A technology of microfluidic chips and manufacturing methods, which is applied in the direction of material analysis, chemical instruments and methods, and laboratory containers through observation of the impact on chemical indicators, and can solve the problem of water quality monitoring anytime, anywhere, and rapid response , High cost of instruments and equipment, relying on professionals, etc., to achieve the effect of fast detection speed, simple production and wide application range

Inactive Publication Date: 2020-09-29
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have problems such as cumbersome steps, time-consuming operation, high cost of instruments and equipment, and reliance on professionals to varying degrees. They are not suitable for on-site rapid water quality analysis, and do not meet the requirements of water quality monitoring (especially regular monitoring and emergency monitoring) anytime and anywhere. , quick response needs

Method used

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  • Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof
  • Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof
  • Paper-based micro-fluidic chip for multi-parameter analysis of water quality detection and manufacturing method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Weigh 24 milligrams (mg) of phenol red, 24 mg of bromothymol blue, and 18 mg of bromocresol green solid powder respectively, and obtain 0.8 g / L of phenol red solution with 30 milliliters (mL) of absolute ethanol as a solvent , 0.8g / L bromothymol blue solution, 0.6g / L bromocresol green solution. Take 2.0 μL of the above three solutions with a pipette gun, drop them on the three ends 1-3 of the “petal” of the chip, dry the solvent, punch holes and cover the film for packaging. Prepare standard solutions with pHs of 4, 5, 6, 7, 8, 9, and 10, respectively. The standard solution was dripped into the center hole of the paper chip to make it completely infiltrated, and the experimental phenomenon was observed ( Figure 2(a)-Figure 2(c) ), read the RGB value of each chip respectively, and make the pH standard color card ( Figure 3(a)-Figure 3(c) ). Depend on figure 1 and Figure 2(a)-Figure 2(c) It can be seen that the phenol red channel has an obvious color...

Embodiment 2

[0039] Embodiment 2: preparation phosphate standard solution: 0 mg / L (mg / L), 2.5mg / L, 5mg / L, 10mg / L, 25mg / L, 50mg / L, 100mg / L, 200mg / L, 400mg / L. Prepare the chip developer: a mixed solution of 20.0g / L sodium molybdate, 8.0g / L Metol, and 30.0g / L sodium sulfite. Prepare A reagent: 1:3 sulfuric acid solution; B reagent: 2.0% antimony potassium tartrate solution. Mix 1 μL antimony potassium tartrate solution (2.0%), 0.8 μL*5 color developer (a mixed solution of 20.0 g / L sodium molybdate, 8.0 g / L metol, and 30.0 g / L sodium sulfite), 0.8 μL sulfuric acid ( 1:3 sulfuric acid solution) was dripped on the flap of the chip from the tip to the root in turn. The phosphate standard solution of different concentrations is excessively dripped on the made chip, and each chip has 6 petals, that is, 6 groups of parallels. The experimental results show that the higher the concentration of the phosphate standard solution, the darker the color ( Figure 4 ). Obtain intuitive picture materials ...

Embodiment 3

[0040] Example 3: Ammonium chloride was dried at 110 degrees Celsius (°C) for 2 hours (h), and ammonium chloride standard ammonia-free solutions of different concentrations were prepared: 0mg / L, 0.2mg / L, 5mg / L, 10mg / L L, 20mg / L, 50mg / L. Preparation of chip developer: the concentration is an ammonia-free reagent solution containing 280 g of sodium salicylate (analytical pure), 50 g of sodium potassium tartrate and 0.40 g of sodium nitroferricyanide per liter. Prepare the activator: dilute it with ammonia-free water and sodium hydroxide solution (2mol / L) to form a sodium hypochlorite solution containing available chlorine concentration 3.5g / L and free alkali concentration 0.75mol / L. Drop 2.0 μL of activator and 2.0 μL of indicator on the flap of the chip from the tip to the root in sequence, and drop excess ammonium chloride standard solution into the center hole of the paper chip to saturate the filter paper with water, and wait for a few seconds. Experimental result shows tha...

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Abstract

The invention provides a novel paper-based multichannel micro-fluidic chip and a manufacturing method thereof, and belongs to the field of rapid analysis and detection. According to the paper-based micro-fluidic chip, a paper-based material is directly cut into a required shape by using an embosser, and the shape is a flower shape with six channels, a pentagram shape, a seven-channel flower shape,an eight-channel flower shape or a sun shape. The chip is manufactured by a stamping method and a laminating method, and multiple reactions of a conventional analysis and detection method can be integrated on one micro-fluidic chip by pre-loading multiple reaction reagents such as an indicator, an activator, and the like. By controlling the positions and the loading capacities of the key reagents, an operator can use simple one-step operation to realize gradual occurrence of multi-step reactions in sequence. The chip and the method can be used for rapidly and simultaneously analyzing variouswater quality detection important parameters such as pH, phosphate, ammonia nitrogen, nitrite and the like, and a detection tool, which is low in price, convenient to operate and rapid in reaction isprovided for water quality monitoring, field rapid detection and the like.

Description

technical field [0001] The invention relates to a paper-based microfluidic chip for multi-parameter analysis of water quality detection and a manufacturing method thereof, belonging to the technical field of rapid analysis and detection. Background technique [0002] With the development of industrial and agricultural production and the expansion of the scope of human life, the consumption and pollution of water resources have become increasingly prominent. In order to ensure the water safety of the public and the smooth progress of industrial and agricultural production, it is necessary to conduct long-term on-site monitoring and random sampling testing of water resources. The commonly used analytical methods for water quality monitoring and water sample detection include titration, electrochemical probe method, colorimetry, ultraviolet-visible spectrophotometry, atomic absorption spectrometry, atomic fluorescence, infrared spectrophotometry, gravimetric method, ion chromat...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/78G01N21/80
CPCB01L3/5027B01L3/502707G01N21/78G01N21/80B01L2200/10B01L2300/08B01L2300/0861B01L2300/126
Inventor 张璐林兆鸿肖絮王璇
Owner CAPITAL NORMAL UNIVERSITY
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