Environment-friendly rapid bactericide
A bactericide, environment-friendly technology, applied in the field of environment-friendly fast bactericide, can solve the problem of stimulating the smell of traditional Chinese medicine
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Embodiment 5
[0051] The present embodiment provides a kind of preparation method of environment-friendly fungicide, comprises the following steps;
[0052] (1) Forsythia in the above-mentioned embodiment 1, magnolia, black cypress leaf, Fangji, sage, Sophora flavescens, Radix radiata, Dianthus leaf, Radix chinensis, Chrysanthemum chrysanthemum, Echinacea purpurea, Patrinia, Radix Paeoniae Rubra, Tribulus terrestris, broccoli, scutellaria baicalensis, phellodendron, white flower, honeysuckle, lovage, phoenix, vitex, bead grass, glutinous rice, thyme, emblica, raspberry were washed, chopped, dried or crushed directly Finally, soak and extract with water enzyme and water-alcohol mixed solvent at a solid-liquid ratio of 1:3-20 (w / v) for 3-22 hours, and filter;
[0053] (2) The liquid obtained in step (1) is respectively passed through the reflux device to reflux heating with a 1:3-20 (v / v) water-alcohol mixed solution, and the components of the plant extract are concentrated to prepare extract...
Embodiment 6
[0055] Embodiment 6 measures the content of alkaloids, terpenes, polyphenols and flavonoids in the fungicide
[0056] The fungicides prepared in Examples 1 to 4 are detected, and the contents of alkaloids, terpenes, polyphenols and flavonoids are measured. The results are shown in Table 2, and the specific methods are as follows:
[0057] The detection method of alkaloids: oxymatrine is used as a standard substance, and acid dye colorimetric method is adopted, the quantitative linear range is 6.8μg / ml~135μg / ml. The operation method is as follows: take 0.2ml of sample, add chloroform to make up to 10ml, add 5ml of acetic acid-sodium acetate buffer solution and 2mL of bromocresol green solution, shake vigorously for 5min, and take 5mL of the lower layer after standing for 1h, 0.01mol / lNaOH-ethanol 1ml, shake well, measure the absorbance at 628nm, the blank control is prepared in the same way with ultrapure water instead of the sample.
[0058] Terpene detection method: oleanol...
Embodiment 7
[0063] Embodiment 7 Bactericidal effect verification
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