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A compound antibacterial agent for preventing and controlling plant diseases

A compound antibacterial and medicament technology, applied in the direction of plant growth regulators, fungicides, biocides, etc., can solve the problems of high drug residues and can not meet the needs of plant pest control, etc., to achieve high bacterial efficiency, mild reaction conditions, Environmentally friendly effect

Active Publication Date: 2021-11-02
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of chemical pesticides will not only cause the bacteria to develop resistance, but also cause high drug residues
With the improvement of people's lives, people's demands for human health, food safety, and environmental safety are becoming stronger and stronger. Therefore, traditional chemical agents can no longer meet the needs of modern agricultural plant pest control. Environmentally friendly new pesticides are of great significance

Method used

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  • A compound antibacterial agent for preventing and controlling plant diseases
  • A compound antibacterial agent for preventing and controlling plant diseases
  • A compound antibacterial agent for preventing and controlling plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Preparation of compound antibacterial agent

[0022] Mix component A and component B uniformly at a weight ratio of 1:4, and then dissolve with a small amount of DMSO as a stock solution.

Embodiment 2

[0023] Embodiment 2: the antibacterial property of compound antibacterial agent to canker sore citrus

[0024] Experimental strain: Canker sorrel citrus.

[0025] The experimental operation steps are as follows:

[0026] (1) Dilute the stock solution of compound antibacterial agent prepared in Example 1 with PBS solution to a final concentration of 330, 100, 33, 10, 3.3, 1, 0.33, 0.1, 0.033 μM, and then use a pipette to pipette each Concentration drug 20 μl, added to a 96-well transparent plate. The same volume of PBS solution was used as blank control.

[0027] (2) Draw 200 μl of bacterial solution (10 6 CFU / ml) was added to each dosing hole, and the solar simulator (wavelength 295-2500 nM, power 80 mW / cm 2 ) for 1 min, the light dose is 4.8 J / cm 2, and then use sterile PBS to dilute each group into different concentration gradients, the dilution gradients are 10×, 100×, 1000× and 10000×, 100000×, take 100 μl and smear it on the pre-configured bacterial culture plate, a...

Embodiment 3

[0032] Embodiment 3: minimum inhibitory concentration of composite antimicrobial agent

[0033] (1) Dilute the compound antibacterial agent with a concentration of 744 μg / ml with NB medium to a final concentration of 372, 186, 93, 46.5, 23.3, 11.7, 5.7, 2.91, 1.44, 0.72, 0.36 μg / ml. Dilute 100 μl, 4000 μg / ml copper sulfate or copper hydroxide stock solution with fresh NB medium to 2000, 1000, 500, 250, 125, 62.5, 31.25, 15.6, 7.8, 3.9 and 1.96 μg / ml The concentration was the positive control. The same volume of PBS solution was used as blank control. Use a pipette gun to pipette 100 μl of each drug concentration into a 96-well plate.

[0034] (2) Draw 100 μl of fresh bacterial solution (10 6 CFU / ml) into each dosing well, and then use a solar simulator (wavelength 295-2500 nM, power 80 mW / cm 2 ) for 1 min, the light dose is 4.8 J / cm 2 .

[0035] (3) After irradiation, continue to culture at 28°C and 200 rpm for 24 hours, the minimum inhibitory concentration of the compo...

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Abstract

The invention belongs to the field of pesticide fungicides, and specifically a composite antibacterial agent for preventing and treating plant diseases. The composite antibacterial agent is prepared by mixing component A and component B according to a certain weight ratio, wherein the weight ratio of component A is 10-50%, the weight proportion of component B is 50-90%, and the sum of the two is 100%. The compound antibacterial agent of the invention has simple preparation process, high yield, convenient storage, rapid sterilization and weakens the drug resistance of pathogenic bacteria, is suitable for plant fungal or bacterial disease prevention and control, and can promote the harmonious development of planting industry and environment.

Description

technical field [0001] The invention belongs to the field of pesticide fungicides, and in particular relates to a compound antibacterial agent for preventing and controlling plant diseases, a preparation method and application thereof. Background technique [0002] Plant bacterial diseases seriously affect the yield and quality of agricultural products, causing serious economic losses. As caused by the bacterial pathogen Xanthomonas spp. ( Xanthomonas citri subsp. citri ( Xcc )) caused by citrus canker is a common bacterial disease in the citrus industry, which can cause the fall of leaves and fruits, and affects the economic value of the citrus industry on a global scale; Huanglongbing is a Gram-negative bacterium of the genus Phloem , not only turn the leaves yellow, but also reduce the product and quality, and in severe cases cause the entire forest to be destroyed; there are other plant diseases such as rice bacterial blight, kiwi fruit canker, tobacco bacterial wilt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N55/08A01N43/90A01N59/16A01P1/00A01P3/00
CPCA01N43/90A01N55/08A01N59/16
Inventor 黄明东蒋利宾刘见永袁彩江龙光徐芃
Owner FUZHOU UNIV